标签：atto试剂

WSE-7130 EzFluoroStain DNA,atto代理,atto试剂

目的和应用

后电泳DNA检测用荧光染料
与EtBr相比，安全性更高
特性

与EtBr(溴化乙缇)相比，在较低的基础上对DNA的灵敏度更高
用紫外线或蓝光检测
bluiled使检测DNA带具有更高的灵敏度和安全性
与EtBr相比，安全性更高
Ames试验最低值，不含IARC致癌物质
无需分离步骤快，废液量小

Specifications

WSE-7130 EzFluoroStainDNA
Major components Fluorescent staining dye
Package Solution 500 μL/bottle
Preparation 10,000x dilute with TAE, TBE buffer and so on
Applicable amount 5~10 μL/50~100 mL buffer, For 50~100 gels
Detection Excitation light: 260~370 nm (UV), 440~500 nm (Blue LED)
Peak of absorption wavelength: 270 nm, 370 nm, 497 nm
Fluorescence: Max absorption wavelength 522 nm
Filter: 500~550 nm LP filter
Storage 1 year at -20℃ in dark (unopened)

Ordering Information

Code No. Description Unit
2332395 WSE-7130 EzFluoroStain DNA 1 pk

WSE-7051 EzRun TBE,atto代理,atto试剂

Purpose and Application

  • Buffer for electrophoresis of DNA

Features

  • Sterilized, DNase free
  • Suitable for analyzing low molecular weight DNA fragments
  • Storable for 1 year at room temperature

 

琼脂糖凝胶电泳所用缓冲液的类型可由DNA片段的大小和迁移后实验的含量来决定。

如果你想分离大于或等于15,000 bp的大DNA，使用TAE缓冲液。如果你不想提取DNA，或者不关心迁移后1000bp或更小的DNA的恢复率，可以使用TBE缓冲液。

上图显示了EzDNA Ladder应用于使用EzRunTAE或EzRunTBE缓冲液的1.5%琼脂糖凝胶，并使用dive – mini在150V下电泳20分钟的结果。可以看到，TAE缓冲液适用于聚合物分离，TBE缓冲液适用于低分子分离。

Specifications

WSE-7051 EzRun TBE
Major components Tris, Boric acid, EDTA, 10x concentrated solution
Package 500 mL/bottle
Preparation 10x dilute with distilled water
Applicable amount For 5L, 220 mL per 1 run (About 22 runs with ATTO Submerge Mini chamber)
Storage For 1 year at room temperature (unopened)

Ordering Information

Code No. Description Unit
2332392 WSE-7051 EzRun TBE 1 pk

 

WSE-7055 EzRun TG,atto代理,atto试剂

WSE-7055 EzRun TG,atto代理,atto试剂目的和应用
蛋白质原生page电泳缓冲液

DNA预制凝胶电泳缓冲液(PAGEL系列)

缓冲液用于罐吸墨
WSE-7055 EzRun TG,atto代理,atto试剂特性
缓冲区的native-PAGE

DNA预制凝胶缓冲液(PAGEL系列)

用于蛋白质蛋白印迹缓冲液

消毒:DNase免费

 

WSE-7055 EzRun TG
Major components Tris,Glycine
Package 500 mL/bottle (10x concentrated)
Preparation Dilute 10 times with distilled water
Applicable amount 5L
Storage 1 year at room temperature
ordering Information
Code No. Description Unit
2332323 WSE-7055 EzRun TG 1 pk

WSE-7050 EzRun TAE,atto代理,atto试剂

Purpose and Application

  • General buffer for electrophoresis of DNA

Features

  • Available for electrophoresis of DNA
  • Sterilized, DNase free
  • Stable for 1 year at room temperature

琼脂糖凝胶电泳所用缓冲液的类型可由DNA片段的大小和迁移后实验的含量来决定。

如果你想分离大于或等于15,000 bp的大DNA，使用TAE缓冲液。如果你不想提取DNA，或者不关心迁移后1000bp或更小的DNA的恢复率，可以使用TBE缓冲液。

上图显示了EzDNA Ladder应用于使用EzRunTAE或EzRunTBE缓冲液的1.5%琼脂糖凝胶，并使用dive – mini在150V下电泳20分钟的结果。可以看到，TAE缓冲液适用于聚合物分离，TBE缓冲液适用于低分子分离。

Specifications

WSE-7050 EzRun TAE
Major components Tris, Acetic acid, EDTA, 50x concentrated solution
Package 200 mL/bottle
Preparation 50x dilute with distilled water
Applicable amount For 25L, 220 mL per 1 run (About 110 runs with ATTO Submerge Mini chamber)
Storage For 1 year at room temperature (unopened)

Ordering Information

Code No. Description Unit
2332391 WSE-7050 EzRun TAE 1 pk

WSE-7040 EzApply DNA,atto代理,atto试剂

Purpose and Application

  • Loading dye for DNA electrophoresis

Features

  • High visibility of red and blue dyes
  • Faster migration of red dye compared to Orange G
  • Sharp DNA bands (compared to Sucrose and/or glycerol)
  • Easy apply
  • Sterilized, Dnase free
  • Storable for 1 year at cold storage (unopened)

 

Dye 1% Agarose / TAE 2% Agarose / TAE
BPB About 400 bp About 150 bp
Red Less than about 50 bp Less than about 10 bp

Migration changes depending to buffer and the kind of agarose.

 

Specifications

WSE-7040 EzApply DNA
Major components Buffer, Ficoll, Dye (BPB, Red)
Package 10 mL, 6x concentrated loading dye
Applicable amount For 50 mL
Preparation Mix EzApply DNA to sample in 5:1 ratio
Storage 1 year at cold storage (unopened)

Ordering Information

Code No. Description Unit
2332394 WSE-7040 EzApply DNA 1 pk

目的和应用

DNA电泳加载染料
特性

红色和蓝色染料的高可见度
红色染料比橙色染料迁移更快
清晰的DNA条带(与蔗糖和/或甘油相比)
简单的应用
消毒,Dnase免费
可在冷库储存一年(未开封)

 

WSE-7310 EzGel Ace,atto代理,atto试剂

Purpose and Application

  • Gel casting buffer for polyacrylamide gel electrophoresis
  • Suitable for separation and stacking gel
  • A gel made of this buffer is preservable for a long term (1 month at 4℃)

Features

  • Completion of electrophoresis within 30min at 300V c.v.
  • No smile effect
  • Similar to the migration pattern of laemmli gel
  • A gel made of this buffer is preservable for a long term (1 month at 4℃)
  • Suitable for SDS-PAGE, native-PAGE and DNA PAGE
  • Storable for 1 year at room temperature

 

Specifications

WSE-7310 EzGel Ace
Major components Tris etc.
Package 250 mL/bottle
Applicable amount for 1 L of gel casting solution (about 100 sheets as ATTO Mini-Slab gel)
Storage 1 year at room temperature (unopened)

Ordering Information

Code No. Description Unit
2332327 WSE-7310 EzGel Ace 1 pk

 

Result Data (pic2)

Sample: Molecular weight marker
EzProtein Ladder, EzStandard

Running buffer: EzRun (25 mM Tris, 192 mM Glycine, 0.1% SDS)
EzRun MOPS (Tris-MOPS, SDS)

Condition: EzGel Ace approx. 30 min
e-PAGEL, EzGel Sep&Stack approx. 75 min

Staining: EzStain AQua (CBB staining)

目的和应用

聚丙烯酰胺凝胶电泳用凝胶铸造缓冲液
适用于凝胶的分离和堆积
用这种缓冲液制成的凝胶可长期保存(在4℃下保存1个月)。
特性

在300V电流下30分钟内完成电泳。
没有微笑的效果
与laemmli凝胶的迁移模式相似
用这种缓冲液制成的凝胶可长期保存(在4℃下保存1个月)。
适用于SDS-PAGE, native-PAGE和DNA PAGE
在室温下可储存1年

WSE-7150 EzGel Sep,atto代理,atto试剂

Purpose and Application

  • Separating gel buffer for polyacrylamide gel electrophoresis
  • Available for SDS-PAGE and native-PAGE

Features

  • High quality, reproducibly and economical use
  • Available for SDS-PAGE and native-PAGE
  • Storable for 1 year at room temperature

 

Specifications

WSE-7150 EzGel Sep
Major components 1.5 M Tris-HCl pH 8.8, 4x concentrated solution
Package 250 mL/bottle
Applicable amount for 1 L of gel casting solution (about 110 sheets as ATTO Mini-Slab gel)
Storage 1 year at room temperature (unopened)

EzGel Sep doesn’t include SDS, but SDS-PAGE is available with using Running buffer including SDS.

Ordering Information

Code No. Description Unit
2332328 WSE-7150 EzGel Sep 1 pk

目的和应用

聚丙烯酰胺凝胶电泳分离凝胶缓冲液
可用于SDS-PAGE和本机page
特性

质量好，重复性好，使用经济
可用于SDS-PAGE和本机page
在室温下可储存1年

WSE-7155 EzGel Stack,atto代理,atto试剂

Purpose and Application

  • Stacking gel buffer for gel polyacrylamide electrophoresis
  • Available for SDS-PAGE and native-PAGE

Features

  • Stacking gel buffer for gel polyacrylamide electrophoresis
  • Available for SDS-PAGE, native-PAGE
  • Storable for 1 year at room temperature

 

Specifications

WSE-7155 EzGel Stack
Major components 0.5 M Tris-HCl pH 6.8, 4x concentrated solution
Package 250 mL/bottle
Applicable amount for 1 L of gel casting solution (about 330 sheets as ATTO Mini-Slab gel)
Storage 1 year at room temperature (unopened)

EzGel Stack doesn’t include SDS, but SDS-PAGE is available with using Running buffer including SDS.

Ordering Information

Code No. Description Unit
2332329 WSE-7155 EzGel Stack 1 pk

目的和应用

凝胶聚丙烯酰胺电泳的叠置凝胶缓冲液
可用于SDS-PAGE和本机page
特性

凝胶聚丙烯酰胺电泳的叠置凝胶缓冲液
可用于SDS-PAGE，本机page
在室温下可储存1年

WSE-7065 EzRun MOPS,atto代理,atto试剂

Purpose and Application

  • Buffer for SDS-PAGE electrophoresis
  • Buffer for fast electrophoresis system

Features

  • High-speed electrophoresis
    Approximately 8x9cm Mini gel: 20-25 min
    Approximately 6x6cm Compact gel: 10-15min
  • High resolution of separation for low MW protein bands
  • Suitable for precast gel & hand cast gel

Specifications

WSE-7065 EzRun MOPS
Major components Tris, MOPS, SDS 20x concentrated solution
Package 1L/bottle
Preparation 20x dilute with distilled water
Applicable amount 500 mL per 1 run (40 runs with ATTO Mini Slab chamber)
Storage For 8 months at room temperature (unopened)

Ordering Information

Code No. Description Unit
2332324 WSE-7065L EzRun MOPS, 1 L 1 pk

 

目的和应用

用于SDS-PAGE电泳的缓冲

快速电泳系统用缓冲液

特性

高速电泳
大约8x9cm的迷你凝胶:20-25分钟
约6x6cm致密凝胶:10-15分钟
高分辨率分离低分子量蛋白带
适用于预制凝胶和手工铸造凝胶

AE-1415 EzRunT,atto代理,atto试剂

AE-1415 EzRunT

Purpose and Application

  • SDS-PAGE electrophoresis buffer for low molecular weight protein and polypeptide separation.

Features

  • Simple preparation: Dissolve in Distilled Water
  • Powder material: Easy keeping and carry
  • Simple and short time electrophoresis: Using with ATTO precast gel, p-PAGEL or cp-PAGEL (for low molecular protein)

Specifications

AE-1415 EzRunT
Major components Tris, Tricine, SDS
Package Powder/bag
Preparation 10X stock solution: Dissolve EzRunT 1 pack in 500 mL distilled water
Applicable amount Max 5 L
Storage 1 year at room temperature (unopened)

Ordering Information

Code No. Description Unit
2332325 AE-1415 EzRunT 1 pk

目的和应用

用于分离低分子量蛋白质和多肽的SDS-PAGE电泳缓冲液。
特性

制备方法简单:溶于蒸馏水
粉状材料:便于保管和携带
简单短时间电泳:使用ATTO预制凝胶，p-PAGEL或cp-PAGEL(用于低分子蛋白质)

AE-1412 EzRun C+,atto代理,atto试剂

AE-1412 EzRun C+

Purpose and Application

  • Separation of Proteins

Features

  • Prevent protein oxidation (recombination of -SH groups) during electrophoresis, providing more accurate results and sharper bands
  • Easily prepared by dissolving with distilled water
  • Powder form saves space and allows long period storage

Specifications

AE-1412 EzRun C+ AE-1412S EzRun C+
Major components Tris, Glycine, SDS and SH protective agent
(final concentrations of 25 mM Tris, 192 mM glycine, 0.1% SDS, and SH-protective agent)
Package Powder/bag x 10 bags Powder/bag x 20 bags
Preparation Dissolve powder in distilled water 500 mL Dissolve powder in distilled water 200 mL
Applicable amount 500 mL x 10 times 200 mL x 20 times
Storage 1 year at room temperature (unopened)

Ordering Information

Code No. Description Unit
2332320 AE-1412 EzRunC+ 1 pk

目的和应用

蛋白质的分离
特性

防止电泳过程中的蛋白质氧化(-SH基团的重组)，提供更准确的结果和更清晰的条带
用蒸馏水溶解容易配制
粉末形式节省空间，并允许长时间储存

AE-1410 EzRun,atto代理,atto试剂

AE-1410 EzRun

Purpose and Application

  • Separation of Proteins

Features

  • Easily prepared by just dissolving with distilled water
  • Powder form saves space and allows long storage
  • Prepared to your desired volume and concentration
  • Low running cost

Specifications

AE-1410 EzRun
Major components Tris, glycine, and SDS
(final concentrations of 25 mM Tris, 192 mM Glycine and 0.1% SDS)
Package Powder/bag
Applicable amount Serve 10 L (20 runs with ATTO Mini Slab Chamber)
Preparation Dissolve powder with distilled water to a final volume of 1L (10X stock solution)
Storage 2 years at room temperature (powder)
6 months at room temperature (solution)
AE-1411 EzRun
Major components 25 mM Tris, 192 mM Glycine and 0.1% SDS
Package Solution/pk
Volume 5 L
Preparation Ready-to-Use
Storage 6 months at room temperature (unopened)

Ordering Information

Code No. Description Unit
2332310 AE-1410 EzRun 1 pk

目的和应用

蛋白质的分离
特性

只需用蒸馏水溶解即可
粉末形式节省空间，并允许长时间存储
准备好你想要的体积和浓度
低运行成本

AE-1450 EzStandard PrestainBlue,atto代理,atto试剂

AE-1450 EzStandard PrestainBlue

EzStandard PrestainBlue是一种预染色(彩色)分子量标记，设计用于蛋白质的SDS-PAGE和印迹。电泳过程中可见蓝色的条带。在印迹过程中，可以确认条带从凝胶迁移到膜上，而无需蛋白染色。

Purpose and Application

  • EzStandard PrestainBlue is a prestained (colored) molecular weight marker designed for SDS-PAGE of protein and blotting. The band is visualized in blue during electrophoresis. During blotting, you can confirm the migration of the band from the gel to the membrane without protein staining.

Features

  • Bands visualized in color
  • Provide sharper bands
  • Stable after repeated freeze-thaw cycles
  • Ready to use
  • No need for preparation

Specifications

AE-1450 EzStandard PrestainBlue
Major components Protein (6 bands of 17 to 115 kDa), Tris-HCl buffer, and BPB
Package 300 μL, 1 tube
Preparation Ready to use
Method of use Appropriate amount applied to the gel (3~5 μL/lane to Mini-gel)
Applicable amount Approximately 60 lanes when using Mini-gel
Storage 1 year at -20℃ (unopened)

Ordering Information

Code No. Description Unit
2332347 AE-1450 EzStandard PrestainBlue 1 pk

AE-1440 EzStandard,atto代理,atto试剂

Purpose and Application

  • SDS-PAGE Molecular weight marker

Features

  • Produce sharp bands
  • Stable after repeated freeze-thaw cycles
  • Ready to use
  • No need for preparation

Specifications

AE-1440 EzStandard
Major components Protein (6 bands of 14.3 to 97.2 kDa), Tris-HCl buffer, and BPB
Package 500 μL, 1 tube
Preparation Ready to use
Method of use Appropriate amount applied to the gel (3 μL/lane to Mini-gel stained with CBB)
Applicable amount Approximately 160 lanes when using Mini-gel
Storage 1 year at -20℃ (unopened)

Ordering Information

Code No. Description Unit
2332340 AE-1440 EzStandard, 1 tube 1 pk

 

WSE-7020 EzProtein Ladder,atto代理,atto试剂

WSE-7020 EzProtein Ladder

目的和应用

在SDS-PAGE电泳过程中可见三种颜色的条带
在Western blotting中可见三种颜色的条带
特性

三色预染标记适用于电泳和印迹
75 kda;红色,25 kda;绿色,其他;蓝色的
12(13)尖带覆盖范围广，从(~5)10kDa到245kDa
准备使用
经过反复冻融循环后稳定

 

Specifications

WSE-7020 EzProtein Ladder
Major components Marker protein, Buffer
Approximate M.W.: 245, 180, 140, 100, 75 (RED), 60, 45, 35, 25 (GREEN), 20, 15, 10, 5 kDa
Package 250 μL x 2 tubes
Applicable amount 1.5 ~ 5 μL/lane (Electrophoresis: 3~5 μL, Blotting: 1.5~3 μL)
Storage 1 year at -20℃ (unopened)

Ordering Information

Code No. Description Unit
2332346 WSE-7020 EzProtein Ladder 1 pk

 

WSE-7025 EzStandard LMW,atto代理,atto试剂

Purpose and Application

  • Low molecular weight protein marker (about 1,000~29,000 Da) for SDS-PAGE

Features

  • sharp and clear band
  • Wide molecular weight range 1,000Da ~ 29,000Da
  • Store for one year and refrigerate for six months.

 

Tips for Low Molecular Weight Protein Electrophoresis

Electrophoresis of proteins and polypeptides with a molecular weight of around 20,000 Da or less is difficult to separate in the usual SDS-PAGE (Laemmli method), so the electrophoresis is carried out with tris-tricin buffer (AE-1415 EzRunT).

Gels and electrophoresis buffers are used exclusively for low molecular weight protein separation. The minimum time for the migration is about 65 minutes, and the migration is completed faster than before.

Low molecular weight proteins and polypeptides can spread if it is not fixed immediately after migration or not stained for a short period of time. CBB staining with AE-1340 EzStain AQua can be stained and destained for a short time using the microwave oven method.

Specifications

WSE-7025 EzStandard LMW
Components 6 protein and polypeptides
1000, 3500, 6500, 14300, 17000, 29000 Da
Package Peptide stock (20x) 0.1 mL
Sample buffer (2x) 10 mL
DTT 150 mg
Preparation Mix Peptide stock, Sample buffer, and DTT and heat at 70℃ for 5-10 minutes before use
Applicable amount approximately 400 to 1,000 times (2~5 μL/well)
Storage 1 year at -20℃, 6 months at 4℃ (after preparation)

Ordering Information

Code No. Description Unit
2332348 WSE-7025 EzStandard LMW 1 set

目的和应用

用于SDS-PAGE的低分子量蛋白质标记(约1000 ~ 29000 Da)
特性

清晰锋利的带子
宽分子量范围1000da ~ 29000da
储存一年，冷藏六个月。
低分子量蛋白质电泳提示

分子量在20,000 Da左右的蛋白质和多肽在通常的SDS-PAGE (Laemmli法)中难以分离，因此采用tris-tricin缓冲液(AE-1415 EzRunT)进行电泳。

凝胶和电泳缓冲液专门用于低分子量蛋白质分离。迁移的最短时间约为65分钟，迁移完成速度较快。

低分子量蛋白质和多肽如果在迁移后不立即固定或短时间内不染色，就会扩散。AE-1340 EzStain AQua的CBB染色可以用微波炉法进行短时间的染色和着色。

WSE-7035 EzStandard HMW,atto代理,atto试剂

WSE-7035 EzStandard HMW

Purpose and Application

  • High molecular weight protein marker (about 29,000~770,000 Da) for SDS-PAGE

Features

  • sharp and clear band
  • Wide molecular weight range 29,000Da ~ 770,000Da
  • Ready-to-Use
  • Store for one year at -20℃ (freezing and thawing up to 5 times)

 

Specifications

WSE-7035 EzStandard HMW
Major Components 11 proteins (29, 45, 66, 77, 97, 116, 140, 170, 220, 270, 770 kDa)
SDS, Glycerol, BPB
Package 100 μL × 4 tubes
Preparation Ready-to-Use
Applicable amount CBB staining detection: 3~5 μL/well (approximately 100 times)
Silver staining detection/Blotting: 0.5~2 μL/well
Storage 1 year at -20℃ (freezing and thawing up to 5 times)

Ordering Information

Code No. Description Unit
2332343 WSE-7035 EzStandard HMW 1 set

目的和应用

用于SDS-PAGE的高分子量蛋白质标记物(约29,000~770,000 Da)
特性

清晰锋利的带子
宽分子量范围29000 da ~ 770000 da
随时可用的
-20℃保存一年(冻融最多5次)

AE-1430 EzApply,atto代理,atto试剂

Purpose and Application

  • Extraction of Protein and PolyPeptide
  • Sample buffer for SDS-PAGE

Features

  • Use of dithiothreitol (DTT) as a reducing reagent
  • Reduction reaction more secure than 2-ME
  • Less likely to produce false bands than 2-ME
  • No smell like 2-ME (2-mercaptoethanol)
  • Low running cost and simple preparation procedure

Specifications

AE-1430 EzApply
Major components Tris-HCl buffer, 2% SDS, 20% sucrose, BPB, and 100 mM DTT
(final concentrations: about halves of the above figures)
Package Solution/30 mL bottle (EzApply solution)
Solution/5 mL bottle x 5 bottles (DTT)
Preparation EzApply solution is added to DTT bottles and mixed with sample at a ratio of 1:1
Applicable amount 5 mL x 5
Storage 6 months at -20℃ (before dissolved in DTT)
1 week after dissolved in DTT

Ordering Information

Code No. Description Unit
2332330 AE-1430 EzApply 1 pk

目的和应用

蛋白质和多肽的提取
用于SDS-PAGE的示例缓冲区
特性

使用二硫苏糖醇(DTT)作为还原剂
还原反应比2-ME更安全
比2-ME更不容易产生假带
没有2-ME(2-巯基乙醇)的味道
运行成本低，制备过程简单

AE-1435 EzApply 2D Kit,atto代理,atto试剂

AE-1435 EzApply 2D Kit

Purpose and Application

  • Extraction of Proteins from Mammalian cell or tissue cultures
  • 2D electrophoresis sample buffer

Features

  • Separate extraction allows higher protein concentration per gel, spot detection is easy.
  • Reducing reagent, alkylating reagent, washing buffer and stratified solutions are also incorporated into a kit
  • Low running cost, while allowing short-time extraction and easy sample preparation
  • Combination use with AE-1430 EzApply (sample preparation kit for SDS-PAGE) makes it applicable to SDS-PAGE as well as 2-D electrophoresis

 

Specifications

AE-1435 EzApply 2D Kit
Major components Wash buffer: Tris, NaCl
Solution 1: Tris, Ampholine, DTT
Solution 2: Urea, Thiourea, Surfactant
Alkylated reagent: Ampholine, DTT
Stratified solutions: Acrylamide, BPB, Urea
Package Solution/30 mL bottle x 2 (Wash buffer)
Solution/20 mL bottle (Solution 1)
Solution/10 mL bottle (Solution 2)
Solution/1 mL tube (Alkylated reagent)
Solution/500 μL tube (Stratified solution)
Powder/tube (DTT)
Preparation Appropriated amount of solution is applied to tissues or cells, which were lysed and extracted
Applicable amount Extracted from 100 mg of mammalian tissue 20 runs
Storage 6 months at -20℃ (before dissolved in DTT)
1 week after dissolved in DTT

Ordering Information

Code No. Description Unit
2332335 AE-1435 EzApply 2D Kit 1 pk

目的和应用

从哺乳动物细胞或组织培养物中提取蛋白质
二维电泳样品缓冲液
特性

单独提取可使每个凝胶的蛋白质浓度更高，斑点检测容易。
还原试剂、烷基化试剂、洗涤缓冲液和分层溶液也被纳入一个套件
运行成本低，提取时间短，样品制备容易
与AE-1430 EzApply (SDS-PAGE样品制备试剂盒)结合使用，使其适用于SDS-PAGE和二维电泳

WSE-7010 EzLabel FluoroNeo,atto代理,atto试剂

WSE-7010 EzLabel FluoroNeo

Purpose and Application

Visualization of electrophoresis pattern with stain FREE

  • Protein sample preparation for SDS-PAGE
  • Fluorescent labeling for protein and polypeptide
  • Protein electrophoresis (1D, 2D-PAGE)
  • Immunoprecipitation, etc.

Features

  • Immediate visualization protein bands after electrophoresis
    Protein fluorescence is emitted by BlueLED or UV excitation light
    [Ex: 330 (UV), 470 nm, Em: 530 nm]
  • Stain FREE, Saving time, Unnecessary for liquid waste disposal
  • Hardly to detect any differences in protein band mobility
  • Observable the gel with Blue LED without removing glass plate gel holder
  • Observed gel is available for western blotting and protein fluorescence is kept after transfer on membrane
  • Observed gel is available for CBB staining and silver staining
  • Kit includes RIPA Lysis Buffer (free amine buffer), MW marker

 

Specifications

WSE-7010 EzLabel FluoroNeo
Components Sample buffer (5x): 12 mL
Labeling reagent: 10 mg
Reducing agent (DTT): 300 mg
MW marker: 600 μL
RIPA Lysis buffer: 10 mL
Storage 1 year at -20℃
Applicable amount 2,000 samples, 20 μL/sample

Ordering Information

Code No. Description Unit
2332333 WSE-7010 EzLabel FluroNeo 1 pk

目的和应用

无染电泳图形可视化

用于SDS-PAGE的蛋白质样品制备
蛋白质和多肽荧光标记
蛋白质电泳(1D, 2D-PAGE)
免疫沉淀反应等。
特性

电泳后立即可见蛋白条带
蛋白质荧光是由蓝光或紫外线激发光发出的
[例:330 (UV)， 470 nm, Em: 530 nm]
无污渍，节省时间，无需处理液体废物
几乎检测不到任何蛋白带流动性的差异
用蓝色LED可以观察到凝胶，不需要移除玻璃板凝胶夹
观察到的凝胶可用于western blotting，蛋白荧光在膜上转移后保存
观察凝胶可用于CBB染色和银染色
试剂盒包括RIPA裂解缓冲液(游离胺缓冲液)、MW标记

AE-1360 EzStain Silver,atto代理,atto试剂

AE-1360 EzStain Silver

Purpose and Application

  • Detection of Protein and DNA

Features

  • Easily prepared by sampling 1 mL of each reagent and diluting
  • Small containers save space for storage.
  • The absence of glutaraldehyde allows the application to Mass Spectrometry
  • Sensitivity is up to approximately 1 ng of protein and dozens of pg of nucleic acid per band.
  • Staining takes approximately 55 minutes.
  • Low running cost

Specifications

AE-1360 EzStain Silver
Major components S-1: sodium thiosulfate
S-2: silver nitrate
S-3: sodium hydrate
S-4: sodium thiosulfate and formaldehyde
Applicable amount Approximately 50 sheets of mini-gel
Storage 2 years at 4℃ away from light
Package 50 mL/each bottle

Ordering Information

Code No. Description Unit
2332360 AE-1360 EzStain Silver 1 pk

目的和应用

蛋白质和DNA的检测
特性

只需取每一种试剂1毫升，然后稀释即可
小容器节省储存空间。
戊二醛的缺失使其可以应用于质谱分析
敏感性高达约1ng蛋白质和几十pg核酸每个波段。
染色大约需要55分钟。
低运行成本

AE-1310 EzStain Reverse,atto代理,atto试剂

AE-1310 EzStain Reverse

Purpose and Application

  • Protein detection

Features

  • Applicable to SDS-polyacrylamide gel electrophoresis: Whiten the background while keeping the protein band transparent
  • Sensitivity is up to several ng of protein per band (several to 10 times sensitive than Coomassie)
  • Staining time of only 20 to 25 minutes for detection
  • Easy to handle
  • Ease of stain removal facilitates the trimming and recovering of protein bands

 

Specifications

AE-1310 EzStain Reverse
Major components R-1: Boric acid, SDS
R-2: Zinc sulfate
Preparation Ready to use
Place the gel in a tray filled with the appropriate amount of the solution and immersed in each solution in order
Applicable amount Approximately 50 sheets of mini-gel
Storage 2 years at room temperature away from light (unopened)
Package 500 mL/each bottle

Ordering Information

Code No. Description Unit
2332350 AE-1310 EzStain Reverse 1 pk

目的和应用

蛋白质检测
特性

适用于sds -聚丙烯酰胺凝胶电泳:使背景变白，同时保持蛋白带透明
每条带蛋白质的灵敏度可达数纳克(比考马斯氏的灵敏度高数至10倍)
染色时间仅20 ~ 25分钟即可检测
很容易处理
易于去除污渍促进修剪和恢复蛋白质带

AE-1340 EzStain AQUA (CBB),atto代理,atto试剂

AE-1340 EzStain AQUA (CBB)

EzStain AQua是考马斯亮蓝(CBB)染色溶液，用于聚丙烯酰胺凝胶电泳(PAGE)检测的蛋白质的可视化。无需使用有机溶剂或醋酸，可在短时间内在清晰的背景下轻松检测蛋白质条带。

规范

产品编号ae – 1340
产品名称ezstain AQua
主要成分考马斯亮蓝(CBB)、酸、稳定剂
使用方法准备使用
向托盘中倒入适量，浸泡凝胶
大约20张迷你凝胶
在室温下保存1年
包1l /瓶
目的和应用

检测的蛋白质
特性

适用于蛋白质的PAGE
无有机溶剂和醋酸，减少了对废液和异味的担忧
可以使用了。只需将其倒入托盘即可
染色时间只需30 ~ 120分钟即可检测
用蒸馏水变色会产生近乎透明的背景
敏感度高达每条带数纳克的蛋白质