AE-1435 EzApply 2D Kit,atto代理,atto试剂

2022年10月27日 作者 jinpanbio

AE-1435 EzApply 2D Kit

Purpose and Application

  • Extraction of Proteins from Mammalian cell or tissue cultures
  • 2D electrophoresis sample buffer

Features

  • Separate extraction allows higher protein concentration per gel, spot detection is easy.
  • Reducing reagent, alkylating reagent, washing buffer and stratified solutions are also incorporated into a kit
  • Low running cost, while allowing short-time extraction and easy sample preparation
  • Combination use with AE-1430 EzApply (sample preparation kit for SDS-PAGE) makes it applicable to SDS-PAGE as well as 2-D electrophoresis

 

Specifications

AE-1435 EzApply 2D Kit
Major components Wash buffer: Tris, NaCl
Solution 1: Tris, Ampholine, DTT
Solution 2: Urea, Thiourea, Surfactant
Alkylated reagent: Ampholine, DTT
Stratified solutions: Acrylamide, BPB, Urea
Package Solution/30 mL bottle x 2 (Wash buffer)
Solution/20 mL bottle (Solution 1)
Solution/10 mL bottle (Solution 2)
Solution/1 mL tube (Alkylated reagent)
Solution/500 μL tube (Stratified solution)
Powder/tube (DTT)
Preparation Appropriated amount of solution is applied to tissues or cells, which were lysed and extracted
Applicable amount Extracted from 100 mg of mammalian tissue 20 runs
Storage 6 months at -20℃ (before dissolved in DTT)
1 week after dissolved in DTT

Ordering Information

Code No. Description Unit
2332335 AE-1435 EzApply 2D Kit 1 pk

目的和应用

从哺乳动物细胞或组织培养物中提取蛋白质
二维电泳样品缓冲液
特性

单独提取可使每个凝胶的蛋白质浓度更高,斑点检测容易。
还原试剂、烷基化试剂、洗涤缓冲液和分层溶液也被纳入一个套件
运行成本低,提取时间短,样品制备容易
与AE-1430 EzApply (SDS-PAGE样品制备试剂盒)结合使用,使其适用于SDS-PAGE和二维电泳