偶联试剂-Coupling Reagents
Microspheres are routinely coated with ligands such as antibodies, oligonucleotides, and peptides, for use in diagnostics and bioseparations. Carboxyl- and amine-modified microspheres require the use of a chemical linker for activation and covalent immobilization of ligand. DEPC-Carbodiimide (EDAC) and Glutaraldehyde are two of the most common crosslinkers used in bead coating protocols. To purchase as a kit, see our coupling kits.
微球通常涂有配体,如抗体、寡核苷酸和肽,用于诊断和生物分离。羧基和胺修饰的微球需要使用化学接头来激活和共价固定配体。DEPC-碳二亚胺 (EDAC) 和戊二醛是珠粒包衣方案中最常用的两种交联剂。要作为套件购买,请参阅我们的耦合套件。
EDAC is a zero-length crosslinker that is routinely used for the covalent binding of amine-containing ligands to carboxylated microspheres.
Glutaraldehyde is a homobifunctional linker that is suitable for binding amine-containing ligands to amine-modified beads.
Catalog Number | Description | Volume |
---|---|---|
AA010-1 | DEPC – Carbodiimide (EDAC) | 1g |
AA010-5 | DEPC – Carbodiimide (EDAC) | 5g |
AA012 | Glutaraldehyde, EM Grade, 25% | 10 × 10mL (ampoules) |