小珠校准套件
Interest in exosome and microvesicle analysis has grown rapidly in recent years as another way to explore disease states, cancer progression, and for drug discovery and delivery. Due to their small size (30 – 100nm) however, these particles are difficult to detect using traditional methods, and require extra processing to segregate or characterize from debris commonly found in biological samples. Flow cytometry has emerged as a popular method of analysis and sorting for small particle characterization.
近年来，作为探索疾病状态、癌症进展以及药物发现和递送的另一种方式，人们对外泌体和微泡分析的兴趣迅速增长。然而，由于它们的小尺寸（30 – 100nm），这些颗粒难以使用传统方法检测，并且需要额外的处理才能从生物样品中常见的碎片中分离或表征。流式细胞仪已成为一种流行的小颗粒表征分析和分类方法。
| Catalog Number | Description |
|---|---|
| 834 | Nanobead Calibration Kit – 50nm, 100nm |
| 832 | Submicron Bead Calibration Kit – 0.2µm, 0.5µm, 0.8µm |
| 833 | Micron Bead Calibration Kit – 1.0µm, 3.0µm, 6.0µm |
参考
Kong, F, Zhang, L, Wang, H, Yuan, G, Guo, A, Li, Q, & Chen, Z. (2015)。循环微泡的收集、分离和储存方法对流式细胞仪分析的影响。实验和治疗医学，10，2093-2101。
黄 S，侯 HW，Kanias T，等。朝着基于微流体的消耗血液储存过程中积累的僵硬和脆弱的人体红细胞的方向发展。芯片实验室。2015；15(2):448-458。
Østergaard E, Frandsen PL, Sandberg E, (2015)使用流式细胞仪测定 HPV 疫苗的冻伤。生物制品卷。43,4:266–273
Venable AS、Williams RR、Haviland DL、McFarlin BK（2014 年）作为细胞表面抗体和离心技术功能的内皮微粒分析。J Immunol Methods, Vol406,117-123
Fischer S, Cornils K, Speiseder T, Badbaran A, Reimer R, Indenbirken, D, … & Lange, C. (2016)。指示通过细胞外囊泡进行水平 DNA 基因转移。PLoS One, 11(9), e0163665。（目录 832、833，亚微米和微米珠校准套件；目录 CC03N，SureCount 3µm）
尺寸校准标准
Our Size Calibration Standards Kit for flow cytometry is comprised of five populations of undyed microspheres in the range of ~4-11µm. Forward scatter channel (FSC) values may be plotted against reported bead diameter to generate a curve that may be used for estimation of the sizes of cells run at the same instrument settings. The Size Calibration Standards Kits are available in two sizes: B (100 tests) and C (280 tests).
我们用于流式细胞术的尺寸校准标准套件由五组未染色的微球组成，范围约为 4-11µm。前向散射通道 (FSC) 值可针对报告的珠子直径绘制，以生成可用于估计在相同仪器设置下运行的细胞大小的曲线。尺寸校准标准套件有两种尺寸：B（100 次测试）和 C（280 次测试）。
| Catalog Number | Description |
|---|---|
| 829 | Size Calibration Standards Kit |
参考
Mathew G, Timm Jr. EA, Sotomayor P, Godoy A, Montecinos VP, Smith G, Huss WJ (2009) ABCG2 介导的 DyeCycle Violet 外排
定义了良性和恶性前列腺中的侧群。细胞周期，8:7，1053-1061
Obokata H, Kojima K, Yamato M, Okano T, Tsuneda S, Vacanti C, (2011) The Potential of Stem Cells in Adult Tissues Representative of the Three Germ Layers Tissue Eng Part A 17(5-6):607-15