标签：biolegend流式抗体-Brilliant Violet 510 抗人 CD64 抗体

Pricing & Availability

Clone

10.1 (See other available formats)

Regulatory Status

RUO

Workshop

VI MA36

Other Names

FcγRI, FcR I

Isotype

Mouse IgG1, κ

Ave. Rating

Submit a Review

Product Citations

publications

Compare all formats See Brilliant Violet 510™ spectral data

Technical data sheet

Product Details

Reactivity

Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.

Concentration

Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC – Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

反应性
人、狒狒、卷尾猴、黑猩猩、食蟹猴、恒河猴、松鼠猴
抗体类型
单克隆
宿主物种
老鼠
免疫原
人类风湿滑液细胞和纤连蛋白纯化的单核细胞。
公式
磷酸盐缓冲溶液，pH 7.2，含有 0.09% 叠氮化钠和 BSA（原产美国）。
准备
通过亲和层析纯化抗体，并在最佳条件下与 Brilliant Violet 510™ 偶联。
浓度
特定批次（请联系技术支持了解浓度和总 µg 量，如果您有批号，请使用我们的查找工具。）
储存和处理
抗体溶液应在 2°C 至 8°C 之间未经稀释储存，并避免长时间暴露在光线下。不要冻结。
应用
FC – 质量测试

推荐用法
每批这种抗体都经过免疫荧光染色和流式细胞术分析的质量控制测试。对于流式细胞仪染色，建议使用该试剂为 100 µl 染色体积中每百万个细胞 5 µl 或每 100 µl 全血 5 µl。

Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel.
.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI^2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections¹².

Application References(PubMed link indicates BioLegend citation)

1. McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
3. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
4. Holl V, et al. 2004. J. Immunol. 173:6274.
5. Hober D, et al. 2002. J. Gen. Virol. 83:2169.
6. Cho HJ, et al. 2007. Physiol Genomics 149:60.
7. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
8. Bruhns P, et al. 2008. Blood 113:3716. PubMed
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
10. Carter DL, et al. 1999. Cytometry 37:41. (FC)
11. Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
12. Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)

RRID

AB_2562512 (BioLegend Cat. No. 305027)
AB_2563822 (BioLegend Cat. No. 305028)

Antigen Details

Structure

Ig superfamily, type I glycoprotein, 72 kD

Distribution

Monocytes, macrophages, dendritic cells, activated granulocytes

Function

Phagocytosis, ADCC

Ligand/Receptor

IgG receptor

Cell Type

Dendritic cells, Granulocytes, Macrophages, Monocytes

Biology Area

Immunology, Innate Immunity

Molecular Family

CD Molecules, Fc Receptors

Antigen References

1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.

Gene ID

2209 View all products for this Gene ID

UniProt

View information about CD64 on UniProt.org

Related Products

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

Other Formats

View All CD64 Reagents Request Custom Conjugation