biolegend流式抗体,Brilliant Violet 785 抗人 CD64 抗体

2022年3月5日 作者 jinpanbio

Pricing & Availability

Clone
10.1 (See other available formats)
Regulatory Status
RUO
Workshop
VI MA36
Other Names
FcγRI, FcR I
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Brilliant Violet 785™ anti-human CD64 Antibody

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305043 25 tests $195.00

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305044 100 tests $395.00

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DescriptionCD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

说明 CD64 是一种 72 kD 的单链 I 型糖蛋白,也称为 FcγRI 和 FcR I。CD64 是免疫球蛋白超家族的成员,在单核细胞/巨噬细胞、树突状细胞和活化的粒细胞上表达。 表达可以通过干扰素γ 刺激上调。 CD64 结合 IgG 免疫复合物。 它在抗原捕获、IgG/抗原复合物的吞噬作用和抗体依赖性细胞毒性 (ADCC) 中发挥作用。

Product Details

Technical data sheet

Product Details

Reactivity
Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application
FC – Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes
Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections12.
Application References
反应性
人、狒狒、卷尾猴、黑猩猩、食蟹猴、恒河猴、松鼠猴
抗体类型
单克隆
宿主物种
老鼠
免疫原
人类风湿滑液细胞和纤连蛋白纯化的单核细胞。
公式
磷酸盐缓冲溶液,pH 7.2,含有 0.09% 叠氮化钠和 BSA(原产美国)。
准备
浓度
特定批次(请联系技术支持以了解浓度和总 µg 量,如果您有批号,请使用我们的查找工具。)
储存和处理
抗体溶液应在 2°C 至 8°C 之间未经稀释储存,并避免长时间暴露在光线下。不要冻结。
应用
FC – 质量测试
推荐用法
每批这种抗体都经过免疫荧光染色和流式细胞术分析的质量控制测试。对于流式细胞仪染色,建议使用该试剂为 100 µl 染色体积中每百万个细胞 5 µl 或每 100 µl 全血 5 µl。
本产品受 Sirigen Inc. 的所有权的约束,并在 Sirigen Inc. 的许可下制造和销售。购买本产品即向买方传达了一项不可转让的权利,仅可将所购买的产品用于研究目的。本产品不得转售或以任何方式合并到其他产品中进行转售。严禁用于治疗或诊断。本产品受美国专利、未决专利申请和国外同等专利保护。
激发激光
紫色激光 (405 nm)
应用笔记
克隆 10.1 识别 CD64 的 EC3 表位。虽然两者都包含 EC3 结构域,但内部测试表明克隆 10.1 优先结合 CD64A (FcγRIA),而不是 CD64B (FcγRIB)。其他报告的应用(相关格式)包括:阻断人 IgG3 和鼠 IgG2a 与 FcγRI2、5、6、11 的结合以及丙酮固定的冷冻组织切片的免疫组织化学染色 12。
应用参考(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
  3. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  4. Holl V, et al. 2004. J. Immunol. 173:6274.
  5. Hober D, et al. 2002. J. Gen. Virol. 83:2169.
  6. Cho HJ, et al. 2007. Physiol Genomics 149:60.
  7. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
  8. Bruhns P, et al. 2008. Blood 113:3716. PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Carter DL, et al. 1999. Cytometry 37:41. (FC)
  11. Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
  12. Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
RRID
AB_2800779 (BioLegend Cat. No. 305043)
AB_2800780 (BioLegend Cat. No. 305044)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution
Monocytes, macrophages, dendritic cells, activated granulocytes
Function
Phagocytosis, ADCC
Ligand/Receptor
IgG receptor
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.
Gene ID
2209 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

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Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?
Yes

Other Formats

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Description Clone Applications
Biotin anti-human CD64 10.1 FC
FITC anti-human CD64 10.1 FC
PE anti-human CD64 10.1 FC
Purified anti-human CD64 10.1 FC, IHC-F, Block
Alexa Fluor® 488 anti-human CD64 10.1 FC
Alexa Fluor® 647 anti-human CD64 10.1 FC
APC anti-human CD64 10.1 FC
Pacific Blue™ anti-human CD64 10.1 FC
Brilliant Violet 421™ anti-human CD64 10.1 FC
PE/Cyanine7 anti-human CD64 10.1 FC
PerCP/Cyanine5.5 anti-human CD64 10.1 FC
APC/Cyanine7 anti-human CD64 10.1 FC
Brilliant Violet 510™ anti-human CD64 10.1 FC
Purified anti-human CD64 (Maxpar® Ready) 10.1 FC, CyTOF®
PE/Dazzle™ 594 anti-human CD64 10.1 FC
Brilliant Violet 605™ anti-human CD64 10.1 FC
APC/Fire™ 750 anti-human CD64 10.1 FC
PE anti-human CD64 10.1 FC
PE/Dazzle™ 594 anti-human CD64 10.1 FC
FITC anti-human CD64 10.1 FC
TotalSeq™-A0162 anti-human CD64 10.1 PG
Brilliant Violet 711™ anti-human CD64 10.1 FC
Alexa Fluor® 700 anti-human CD64 10.1 FC
Brilliant Violet 785™ anti-human CD64 10.1 FC
TotalSeq™-C0162 anti-human CD64 10.1 PG
Ultra-LEAF™ Purified anti-human CD64 10.1 FC, IHC-F, Block
TotalSeq™-B0162 anti-human CD64 10.1 PG
TotalSeq™-D0162 anti-human CD64 10.1 PG
GMP PE anti-human CD64 10.1 FC
GMP FITC anti-human CD64 10.1 FC