Dojindo,Cellstain-Hoechst 33258溶液/1/H341,Hoechst 染料具有细胞膜渗透性

Staining Procedure1.Prepare 10-50 μM Hoechst dye solution with PBS or an appropriate buffer.^a)2.Add Hoechst dye solution with 1/10 of the volume of cell culture medium to the cell culture.^b)3.Incubate the cell at 37ºC for 10-20 min.4.Wash cells twice with PBS or an appropriate buffer.5.Observe the cells under a fluorescence microscope with 350 nm excitation and 460 nm emission filters.a) Since Hoechst dyes may be carcinogenic, extreme care is necessary during handling.b) Or you may replace the culture medium with 1/10 concentration of Hoechst dye buffer solution.

1. M. J. Lydon, et al., Vital DNA Staining and Cell Sorting by Flow Microfluorometry. J Cell Physiol. 1980;102:175-181.2. M. Sriram, et al., Structural Consequences of a Carcinogenic Alkylation Lesion on DNA: Effect of O6-ethylguanine on the Molecular Structure of the d(CGC[e6G]AATTCGCG)-netropsin Complex. Biochemistry. 1992;31:11823-11834.3. Y. Tadokoro, et al., Characterization of Histone H2A.X Expression in Testis and Specific Labeling of Germ Cells at the Commitment Stage of Meiosis with Histone H2A.X Promoter-Enhanced Green Fluorescent Protein Transgene. Biol Reprod. 2003;69:1325-1329.4. F. Wada, et al., Analyses of Expression and Localization of Two Mammalian-Type Transglutaminases in Physarum polycephalum, an Acellular Slime Mold. J Biochem. 2004;136:665-672.5. T. Ohara, et al., FoSTUA, Encoding a Basic Helix-Loop-Helix Protein, Differentially Regulates Development of Three Kinds of Asexual Spores, Macroconidia, Microconidia, and Chlamydospores, in the Fungal Plant Pathogen Fusarium oxysporum. Eukaryot Cell. 2004;3:1412-1422.