Dojindo,Cellstain-DAPI/1/D212,DAPI 是一种 AT 序列特异性 DNA 嵌入剂

Staining Procedure1.Prepare 10-50 μM DAPI solution with PBS or an appropriate buffer.^a)2.Add DAPI solution with 1/10 of the volume of cell culture medium to the cell culture.^b)3.Incubate the cell at 37^oC for 10-20 min.4.Wash cells twice with PBS or an appropriate buffer.5.Observe the cells using a fluorescence microscope with 360 nm excitation and 460 nm emission filters.

a) Since DAPI may be carcinogenic, extreme care is necessary during handling.b) Or you may replace the culture medium with 1/10 concentration of DAPI buffer solution.

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