Reagents > WesternSure® Pre-stained Chemiluminescent Protein Ladder,WesternSure® 预染色化学发光蛋白阶梯
Western blot workflows with chemiluminescent protein ladders are prone to complications:
- Your ladder may require strep-tagged HRP
- Your ladder may rely on IgG binding sites to be detected by an HRP secondary antibody
- Trial and error may be required to optimize loading volume for your ladder
Note: The WesternSure Ladder is not recommended for use when stripping and reprobing Western blots. Stripping buffer alters the chemiluminescent functionality of the ladder, resulting in weak or no signal.
带有化学发光蛋白梯的蛋白质印迹工作流程容易出现并发症：
您的梯子可能需要带链球菌标记的 HRP
您的梯子可能依赖于 HRP 二抗检测到的 IgG 结合位点
可能需要反复试验来优化梯子的装载量
注意：剥离和重新检测蛋白质印迹时不建议使用 WesternSure Ladder。 剥离缓冲液会改变梯子的化学发光功能，导致信号微弱或无信号。
Remove Complications from Your Chemiluminescent Western Workflow
The WesternSure chemiluminescent protein ladder is the only pre-stained, multi-colored protein ladder for both film and digital chemiluminescence detection.
- Fits with your current protocol without optimization
- Does not rely on strep-tagged HRP or secondary antibody binding
- Works with a variety of substrates
- Provides a vibrant color scheme that allows you to quickly orient your membrane
Not sure which protein ladder to choose? Learn more about how to choose the right protein ladder.
WesternSure 化学发光蛋白分子量标准是唯一一种用于胶片和数字化学发光检测的预染色、多色蛋白分子量标准。
适合您当前的协议，无需优化
不依赖链球菌标记的 HRP 或二抗结合
适用于各种基材
提供充满活力的配色方案，可让您快速定位膜
不确定选择哪种蛋白质阶梯？ 详细了解如何选择正确的蛋白质阶梯。
Components
- Nine recombinant protein bands (8 to 260 kDa)
- One green reference band (8 kDa)
- Three orange reference bands (38, 70, and 260 kDa)
- 九个重组蛋白条带（8 至 260 kDa）
一个绿色参考带 (8 kDa)
三个橙色参考波段（38、70 和 260 kDa）
Images
