Reagents > IRDye® 680RD Donkey anti-Guinea Pig IgG Secondary Antibody
Immunogen
Guinea Pig IgG, whole molecule
Purity and Specificity
Isolation of specific antibodies was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule guinea pig IgG, and with the light chains common to other guinea pig immunoglobulins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications.
使用固定在琼脂糖珠上的抗原，通过免疫亲和层析分离特异性抗体。 基于免疫电泳和/或 ELISA，该抗体与全分子豚鼠 IgG 以及其他豚鼠免疫球蛋白共有的轻链发生反应。 该抗体通过 ELISA 和/或固相吸附测试，以确保与牛、鸡、山羊、叙利亚仓鼠、马、人、小鼠、兔、大鼠和绵羊血清蛋白的交叉反应最小。 该偶联物经过专门测试，可用于蛋白质印迹和 In-Cell Western™ 检测应用。
Applications
Highly recommended for:
- Western Blot
- In-Cell Western Assay
- On-Cell Western Assay
- Protein Array
- Immunohistochemistry
- Small Animal Imaging
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
- 蛋白质印迹
细胞内西方检测
On-Cell Western 检测
蛋白质阵列
免疫组织化学
小动物成像
显微镜
2D 凝胶检测
组织切片成像
Formulation
IRDye 680RD secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
IRDye 680RD 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。
复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Odyssey® Western blot detection | 1:15,000 | 1:5,000 – 1:25,000 |
| In-Cell Western assay detection | 1:800 | 1:200 – 1:1,200 |
| Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
RRID
- P/N 925-68077: RRID AB_2814914
- P/N 926-68077: RRID AB_10956079