标签：CellVue® Fluorescent Cell Labeling Kits

CellVue® Fluorescent Cell Labeling Kits,CellVue® 荧光细胞标记试剂盒

CellVue® Fluorescent Cell Labeling Kits

CellVue® Burgundy Fluorescent Cell Labeling Kit,CellVue® 酒红色荧光细胞标记试剂盒

CellVue® Fluorescent Cell Labeling Kits

CellVue® NIR815 Fluorescent Cell Labeling Kit,CellVue® NIR815 荧光细胞标记试剂盒

CellVue® Fluorescent Cell Labeling Kits

Diluent C for Use with CellVue® Fluorescent Cell Labeling Kits,用于 CellVue® 荧光细胞标记试剂盒的稀释剂 C

Fluorescently Label Cells or Tissues for in vivo or in vitro Studies

CellVue fluorescent imaging kits use proprietary labeling technology to stably incorporate fluorescent dyes containing long aliphatic hydrocarbon tails into lipid membranes1. They are useful for researchers working in all aspects of science and technology where fluorescently-labeled cells and/or tissues are required. CellVue dyes also provide researchers with valuable tools for many in vivo and in vitro cell studies using fluorescent membrane labels.

The CellVue dyes are provided in an easy-to-use kit format containing a solution of the dye in ethanol and a cell labeling diluent. The labeling diluent, Diluent C, provided with the kit is an iso-osmotic aqueous solution that contains no physiologic salts or buffers, detergents, or organic solvents, and is designed to maintain cell viability while maximizing dye solubility and staining efficiency2, 3. The simple membrane labeling protocol provided with the kit is applicable to almost any type of cell or organism.

CellVue 荧光成像试剂盒使用专有的标记技术将含有长脂肪烃尾的荧光染料稳定地结合到脂质膜中1。 它们对于在需要荧光标记的细胞和/或组织的科学和技术的各个方面工作的研究人员非常有用。 CellVue 染料还为研究人员提供了许多使用荧光膜标记进行体内和体外细胞研究的宝贵工具。

CellVue 染料以易于使用的试剂盒形式提供，其中包含染料乙醇溶液和细胞标记稀释剂。 试剂盒随附的标记稀释剂 Diluent C 是一种等渗水溶液，不含生理盐或缓冲液、去污剂或有机溶剂，旨在保持细胞活力，同时最大限度地提高染料溶解度和染色效率 2, 3。 试剂盒提供的简单膜标记方案适用于几乎任何类型的细胞或生物体。

Molecular Structure of CellVue Dyes

CellVue dyes consist of long aliphatic hydrocarbon tails linked to a polar fluorescent chromophore.

  • These extremely lipophilic fluorescent dyes rapidly and stably integrate into the phospholipid membrane of cells or other membrane-containing bioparticles by non-covalent interactions.
  • The dyes are stably maintained within the lipid bilayer through strong hydrophobic interactions and do not transfer into the unstained membranes of adjacent cells, which permits a labeled cell to be tracked for extended periods of time.
  • The rapid incorporation of the dye allows for immediate analysis of cell functions without a waiting period.
  • 这些极具亲脂性的荧光染料通过非共价相互作用快速而稳定地整合到细胞的磷脂膜或其他含膜生物颗粒中。
    染料通过强疏水相互作用稳定地保持在脂质双层内，并且不会转移到相邻细胞的未染色膜中，这允许长时间跟踪标记的细胞。
    染料的快速掺入允许立即分析细胞功能而无需等待。

References

  1. Horan, P. K., and Slezak, S. E., Nature, 340, 167-168 (1989).
  2. Horan, P. K., et al., Methods Cell Biol., 33, 469-490 (1990).
  3. Poon, R.Y., et al. In Living Color: Flow Cytometry and Cell Sorting Protocols, Diamond, R. A., and DeMaggio, S. (Eds.). p. 302-352 (Springer-Verlag, New York, 2000).