Dojindo,R-藻红蛋白标记试剂盒-NH2/3/LK23,藻胆蛋白是源自蓝细菌和真核藻类的荧光蛋白

Product Description
藻胆蛋白是源自蓝细菌和真核藻类的荧光蛋白。 它们的荧光远高于荧光素和罗丹明等化学荧光探针。 R-藻红蛋白（R-PE）是一种藻胆蛋白，在578 nm左右发出红色荧光，在488 nm处可被激发（图1）。 由于这种高荧光，藻胆蛋白标记的抗体和其他分子可以在流式细胞术和免疫染色中提供更高的灵敏度。 R-藻红蛋白标记试剂盒-NH2 用于简单快速地制备 R-PE 标记的 IgG（图 2）。 NH2-reactive R-PE（本试剂盒的一个组成部分）具有活化的酯基，无需任何活化过程即可轻松与目标分子的氨基形成共价键。 该套件中的过滤管允许快速更换缓冲液并浓缩样品 IgG 溶液。 该试剂盒包含 R-PE 标记所需的所有试剂，包括偶联物的储存缓冲液。
 

Precaution♦ 使用本试剂盒标记的蛋白质的分子量应大于 50,000。♦ 标记过程中，IgG 或 R-藻红蛋白结合的 IgG 始终在过滤管的膜上。♦ 如果 IgG 溶液中含有其他具有 分子量大于 10,000，例如 BSA 或明胶，在用此试剂盒标记 R-藻红蛋白之前纯化 IgG 溶液。 IgG 溶液可通过 IgG 纯化试剂盒（本试剂盒未提供）进行纯化。♦ 如果 IgG 溶液含有少量不溶性物质，则将溶液离心并使用上清液进行标记。

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Can I use this kit for other proteins?

Yes, if the molecular weight is higher than 50,000, and it has a reactive primary or secondary amino group. Follow the protocol for IgG labeling with 0.5-1 nmol of sample protein.

How many R-PE molecules per IgG are introduced?

The average number of R-PE molecule per IgG is 1 to 2.

Does unconjugated NH2-reactive R-PE still have an activated ester after the labeling reaction to IgG?

No. It is completely hydrolyzed during the reaction.

Does NH2-reactive R-PE form an oligomer during the labeling reaction?

No. Since all amino groups of NH2-reactive R-PE are blocked, no oligomerization is possible.

What is the minimum amount of IgG that can be labeled with LK23-10?

The minimum amount is 50 μg. There is no significant difference in sensitivity and background between 50 μg and 200 μg of IgG.

Do I have to use WS buffer included with the kit?

Yes. Use the WS buffer to prepare a stock solution of the conjugate. However, you can choose any kind of buffer appropriate to dilute the conjugate stock solution for your experiment.

How long is the conjugate stable?

If you store at 4ºC, it is stable for over 2 months. For longer storage, add 100% volume of glycerol, aliquot, and store at -20ºC. However, please note that the stability depends on the protenitself.

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