Dojindo,R-藻红蛋白标记试剂盒-NH2/3/LK23,藻胆蛋白是源自蓝细菌和真核藻类的荧光蛋白
2022年4月30日Precaution♦ 使用本试剂盒标记的蛋白质的分子量应大于 50,000。♦ 标记过程中，IgG 或 R-藻红蛋白结合的 IgG 始终在过滤管的膜上。♦ 如果 IgG 溶液中含有其他具有 分子量大于 10,000，例如 BSA 或明胶，在用此试剂盒标记 R-藻红蛋白之前纯化 IgG 溶液。 IgG 溶液可通过 IgG 纯化试剂盒（本试剂盒未提供）进行纯化。♦ 如果 IgG 溶液含有少量不溶性物质，则将溶液离心并使用上清液进行标记。
1. K.R. McCarthy, A. Watanabe, M. Kuraoka, K.T. Do, C.E. McGee, G.D. Sempowski, T.B. Kepler, A.G. Schmidt, G. Kelsoe and S.C. Harrison, “Memory B Cells that Cross-React with Group 1 and Group 2 Influenza A Viruses Are Abundant in Adult Human Repertoires”, Immunity., 2018, 48, (1), 174.2. K.Y Su, A. Watanabe, C.H. Yeh, G. Kelsoe, M. Kuraoka, “Efficient Culture of Human Naive and Memory B Cells for Use as APCs”, J. Immunol.., 2016, 197, (10), 4163.3. T. Tsujikawa, T. Yaguchi, G. Ohmura, S. Ohta, A. Kobayashi, N. Kawamura, T. Fujita, H. Nakano, T. Shimada, T. Takahashi, R. Nakao, A. Yanagisawa, Y. Hisa, Y. Kawakami, “Autocrine and paracrine loops between cancer cells and macrophages promote lymph node metastasis via CCR4/CCL22 in head and neck squamous cell carcinoma”, Int. J. Cancer., 2013, 132, (12), 2755.4. Y. Inaguma, Y. Akahori, Y. Murayama, K. Shiraishi, S. Tsuzuki-Iba, A. Endoh, J. Tsujikawa, A. Demachi-Okamura, K. Hiramatsu, H. Saji, Y. Yamamoto, N. Yamamoto, Y. Nishimura, T. Takahashi, K. Kuzushima, N. Emi, Y. Akatsuka, “Construction and molecular characterization of a T-cell receptor-like antibody and CAR-T cells specific for minor histocompatibility antigen HA-1H”, Gene Ther.., 2014, 21, (6), 575.
Yes, if the molecular weight is higher than 50,000, and it has a reactive primary or secondary amino group. Follow the protocol for IgG labeling with 0.5-1 nmol of sample protein.
The average number of R-PE molecule per IgG is 1 to 2.
No. It is completely hydrolyzed during the reaction.
No. Since all amino groups of NH2-reactive R-PE are blocked, no oligomerization is possible.
The minimum amount is 50 μg. There is no significant difference in sensitivity and background between 50 μg and 200 μg of IgG.
Yes. Use the WS buffer to prepare a stock solution of the conjugate. However, you can choose any kind of buffer appropriate to dilute the conjugate stock solution for your experiment.
If you store at 4ºC, it is stable for over 2 months. For longer storage, add 100% volume of glycerol, aliquot, and store at -20ºC. However, please note that the stability depends on the protenitself.