biolegend流式抗体-GMP FITC anti-human CD64 Antibody,biolegend 260014

2022年3月5日 作者 jinpanbio

Pricing & Availability

Clone
10.1 (See other available formats)
Workshop
VI MA36
Other Names
FcγRI, FcR I
Isotype
Mouse IgG1, κ
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Product Citations
publications
GMP FITC anti-human CD64 Antibody
Typical results from human peripheral blood monocytes stained either with 10.1 FITC used at 5 µL/test (solid histogram) or with an isotype control (dashed histogram).

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260014 100 tests $200.00

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DescriptionCD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

CD64 是一种 72 kD 的单链 I 型糖蛋白,也称为 FcγRI 和 FcR I。CD64 是免疫球蛋白超家族的成员,在单核细胞/巨噬细胞、树突状细胞和活化的粒细胞上表达。 表达可以通过干扰素γ 刺激上调。 CD64 结合 IgG 免疫复合物。 它在抗原捕获、IgG/抗原复合物的吞噬作用和抗体依赖性细胞毒性 (ADCC) 中发挥作用。

Product Details

Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
200 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application
FC – Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Application Notes
Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections12.
推荐用法
每批这种抗体都经过免疫荧光染色和流式细胞术分析的质量控制测试。 对于流式细胞仪染色,建议使用该试剂为 100 µL 染色体积中每百万个细胞 5 µL 或每 100 µL 全血 5 µL。 建议对试剂进行滴定,以获得每种应用的最佳性能。激发激光
蓝色激光(488 nm)
应用笔记
克隆 10.1 识别 CD64 的 EC3 表位。 虽然两者都包含 EC3 结构域,但内部测试表明克隆 10.1 优先结合 CD64A (FcγRIA),而不是 CD64B (FcγRIB)。 其他报告的应用(针对相关格式)包括:阻断人 IgG3 和鼠 IgG2a 与 FcγRI2、5、6、11 的结合以及丙酮固定冷冻组织切片的免疫组织化学染色 12。
Application References(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
  3. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  4. Holl V, et al. 2004. J. Immunol. 173:6274.
  5. Hober D, et al. 2002. J. Gen. Virol. 83:2169.
  6. Cho HJ, et al. 2007. Physiol Genomics 149:60.
  7. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
  8. Bruhns P, et al. 2008. Blood 113:3716. PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Carter DL, et al. 1999. Cytometry 37:41. (FC)
  11. Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
  12. Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
Disclaimer
GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution
Monocytes, macrophages, dendritic cells, activated granulocytes
Function
Phagocytosis, ADCC
Ligand/Receptor
IgG receptor
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.
Gene ID
2209 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

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Description Clone Applications

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?
Yes

Other Formats

View All CD64 Reagents Request Custom Conjugation

Description Clone Applications
Biotin anti-human CD64 10.1 FC
FITC anti-human CD64 10.1 FC
PE anti-human CD64 10.1 FC
Purified anti-human CD64 10.1 FC, IHC-F, Block
Alexa Fluor® 488 anti-human CD64 10.1 FC
Alexa Fluor® 647 anti-human CD64 10.1 FC
APC anti-human CD64 10.1 FC
Pacific Blue™ anti-human CD64 10.1 FC
Brilliant Violet 421™ anti-human CD64 10.1 FC
PE/Cyanine7 anti-human CD64 10.1 FC
PerCP/Cyanine5.5 anti-human CD64 10.1 FC
APC/Cyanine7 anti-human CD64 10.1 FC
Brilliant Violet 510™ anti-human CD64 10.1 FC
Purified anti-human CD64 (Maxpar® Ready) 10.1 FC, CyTOF®
PE/Dazzle™ 594 anti-human CD64 10.1 FC
Brilliant Violet 605™ anti-human CD64 10.1 FC
APC/Fire™ 750 anti-human CD64 10.1 FC
PE anti-human CD64 10.1 FC
PE/Dazzle™ 594 anti-human CD64 10.1 FC
FITC anti-human CD64 10.1 FC
TotalSeq™-A0162 anti-human CD64 10.1 PG
Brilliant Violet 711™ anti-human CD64 10.1 FC
Alexa Fluor® 700 anti-human CD64 10.1 FC
Brilliant Violet 785™ anti-human CD64 10.1 FC
TotalSeq™-C0162 anti-human CD64 10.1 PG
Ultra-LEAF™ Purified anti-human CD64 10.1 FC, IHC-F, Block
TotalSeq™-B0162 anti-human CD64 10.1 PG
TotalSeq™-D0162 anti-human CD64 10.1 PG
GMP PE anti-human CD64 10.1 FC
GMP FITC anti-human CD64 10.1 FC