Dojindo,Liperflou/50/L248,Liperfluo 是一种含有低聚氧乙烯的苝衍生物

Liperfluo 是一种含有低聚氧乙烯的苝衍生物，由 Dojindo 设计和独家开发，用于检测脂质过氧化物。 Liperfluo 通过在乙醇等有机溶剂中的脂质过氧化物特异性氧化发出强烈的荧光。在检测活性氧 (ROS) 的荧光探针中，Liperfluo 是唯一可以特异性检测脂质过氧化物的化合物。由于氧化的 Liperfluo 的激发和发射波长分别为 524 nm 和 535 nm，因此可以最大限度地减少样品的光损伤和自发荧光。与二异喹啉环一端相连的四甘醇基团有助于其在水性缓冲液中的溶解性和分散性。 Liperfluo 的氧化形式在水性介质中几乎不发荧光，并在细胞膜等亲脂部位发出强荧光。因此，它可以很容易地通过荧光显微镜和活细胞的流式细胞术分析应用于脂质过氧化物成像。 Liperfluo 用于监测铁死亡研究中的脂质过氧化。

Data was kindly provided from Dr. N. Noguchi, Doshisha University, System Life Science Laboratory.

Flow cytometric analysis of lipid hydroperoxides in live cell

Data was kindly provided from Dr. N. Noguchi, Doshisha University, System Life Science Laboratory.

Procedure:1. Prepare cell suspension (2.5 x 10⁵ cell/well) in 35mm Glass bottom dish and incubate at 37^oC overnight in CO₂.2. Discard the media and add new media containing Liperfluo (final conc. 1μM) .3. Incubate at 37^oC for 30 min in CO₂.4. Discard the media add new media containing t-BHP (final conc. 250μM ).5. Incubate at 37^oC for 2 hours in CO₂.6. Observe using confocal microscope.Data was kindly provided from Dr. T. Kumagai and Dr. H. Imai, Kitasato University, School of Pharmacy.

坏死、凋亡和自噬被称为细胞死亡相关过程。 2012 年，Ferroptosis 被提出为新的细胞死亡之一。 铁死亡被研究为由铁离子依赖性过氧化脂质的积累引起的非凋亡性细胞死亡。 Liperfluo用作荧光证明，可以直接检测细胞内过氧化脂质。

Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease.B. R. Stockwell et al., Cell, 2017, 171(2), 273.

Oxidized Arachidonic/Adrenic Phosphatidylethanolamines Navigate Cells to FerroptosisV. E. Kagan et al., Nat. Chem. Biol., 2017, 13, (1), 81.

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