Dojindo,Bacstain-细菌活力检测试剂盒-CTC/DAPI/1/BS09

2022年5月2日 作者 jinpanbio
Detection Principle

-Bacstain-细菌活力检测试剂盒是用于细菌荧光双染的系列产品。通过组合不同类型的荧光染料,可以获得每个指标(膜损伤、呼吸活性和酯酶活性)的染色图像。细菌活力通常通过营养琼脂培养基中的菌落形成来评估。然而,这需要很长的培养时间,并且很难识别有活力但不可培养 (VNC) 的细菌的生长。然而,荧光染色不需要细菌培养,并且可以通过快速和简单的协议进行活力评估。

-Bacstain- 细菌活力检测试剂盒 – CTC/DAPI 以细菌的呼吸活动为指标。 CTC 在被细菌呼吸活动形成的 NAD(P)H 还原后产生甲臜染料,因此选择性地染色活性细菌。 DAPI 是一种特异于 DNA 的 AT 序列的小沟结合剂,可渗透到细菌中以染色核酸,无论膜是否受损。因此,该试剂盒可用于测量具有呼吸活性的细菌与总细菌的比例,用于分析每个污渍的荧光图像。


Quantitative analysis

-Bacstain- Bacterial Viability Detection Kit – DAPI/PI (BS08) and –CTC/DAPI (BS09) were used to evaluate the effect of benzalkonium chloride on S. aureus (Gram-positive bacteria) by fluorescent imaging. The images were quantified by ImageJ and show the correlation with drug concentration.The results showed that the effects of benzalkonium chloride on the respiratory activity and membrane damage of S. aureus varied greatly depending on the indicator detected.The evaluation of multiple indicators will contribute to improving the reliability of drug efficacy measurements by providing a multidimensional view of the activity of the bacterium that would be missed by a single indicator.


Related Product Information

Product Size Product Code
-Bacstain- CFDA solution 100 assays BS03
-Bacstain- DAPI solution 100 assays BS04
-Bacstain- AO solution 100 assays BS05
-Bacstain- PI solution 100 assays BS07
-Bacstain- CTC Rapid Staining Kit (for Flow cytometry) 100 assays BS01
-Bacstain- CTC Rapid Staining Kit (for Microscopy) 100 assays BS02

Dojindo细胞分析

细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。

WST检测机制

 

ß-半乳糖苷酶检测试剂

细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢

应用 产品展示
细胞生长检测,药物筛选,比色/荧光检测 细胞计数试剂盒-8
细胞计数试剂盒8 + 96孔有机硅定向剂
细胞计数试剂盒-F
细胞毒性LDH检测试剂盒
-WST 96孔有机硅定向剂
MTT
了解检测机制的差异: 点击这里
细胞周期分析 细胞周期测定溶液深红色
细胞周期测定溶液蓝色