Dojindo,羧基PTIO/10/C348

2022年5月1日 作者 jinpanbio
Product Description

Carboxy-PTIO 是一种稳定的水溶性有机自由基,可与 NO 反应形成 NO2·此反应可通过电子自旋共振 (ESR) 进行监测。 NO 是一种不稳定的分子,在生物系统中的代谢具有复杂的级联反应。快速产生的 NO 相关代谢物进行各种生理活动。常用的NO清除剂如血红蛋白捕集剂NO;它们还捕获 NOS 抑制剂,例如精氨酸衍生物。这些 NO 清除剂还同时淬灭所有其他与 NO 相关的代谢物。相比之下,Carboxy-PTIO 不会显着影响其他与 NO 相关的产品系统,因为它将 NO 转化为 NO2,NO2 是 NO 的代谢产物。因此,Carboxy-PTIO 可用于研究 NO 与其下游代谢物分开的影响。 Akaike 博士和其他人表明,Carboxy-PTIO 抑制由乙酰胆碱诱导的大鼠主动脉环松弛,其效果是 NG-硝基精氨酸的两倍。 Yoshida 博士和其他人报告说,通过 Carboxy-PTIO 处理产生的 NO 下游代谢物与单独使用 NO 相比具有更高的抗病毒活性。 NO代谢物在生物系统中发挥重要作用;因此,应与 NO 分开调查。

Reaction of NO quenching

1. E. F. Ullman, et al., Studies of Stable Free Radicals. X. Nitronyl Nitroxide Monoradicals and Biradicals as Possible Small Molecule Spin Labels. J Am Chem Soc. 1972;94:7049-7059.2. Y. Miura, et al., Polymers Containing Stable Free Raficals, 5. Preparation of a Polymer Containing Imidazoline 3-Oxide 1-Oxyl Groups. Macromol Chem Phys. 1973;172:233-236.3. K. Inoue, et al., Magnetic Properties of the Crystals of p-(1-Oxyl-3-Oxide-4, 4, 5, 5-Tetramethyl-2-Imidazolin-2-Yl)Benzoic acid and Its Alkali Metal Salts. Chem Phys Lett. 1993;207:551-555.4. T. Akaike, et al., Antagonistic Action of Imidazolineoxyl N-Oxides Against Endothelium-Derived Relaxing Factor/NO Through a Radical Reaction. Biochemistry. 1993;32:827-832.5. J. Joseph, et al., Trapping of Nitric Oxide by Nitronyl Nitroxides: an Electron Spin Resonance Investigation. Biochem Biophys Res Commun. 1993;192:926-934.6. M. Yoshida, et al., Therapeutic Effects of Imidazolineoxyl N-Oxide Against Endotoxin Shock Through Its Direct Nitric Oxide-scavenging Activity. Biochem Biophys Res Commun. 1994;202:923-930.7. T. Az-Ma, et al., Reaction Between Imidazolineoxil N-Oxide(Carboxy-PTIO) and Nitric Oxide Released from Cultured Endothelial Cells:Quantitative Measurement of Nitric Oxide by ESR Spectrometry. Life Sci. 1994;54:PL185-PL190.8. H. Maeda, et al., Multiple Functions of Nitric Oxide in Pathophysiology and Microbiology: Analysis by a New Nitric Oxide Scavenger. J Leukoc Biol. 1994;56:588-592.9. T. Akaike, et al., Quantitation of Nitric Oxide Using 2-Phenyl-4, 4, 5, 5-Tetramethylimidazoline-1-Oxyl 3-Oxide(PTIO). Methods Enzymol. 1996;268:211-221.10. S. Satoh, et al., NO Donors Stimulate Noradrenaline Release from Rat Hippocampus in a Calmodulin-dependent Manner in the Presence of LCysteine. J Cell Physiol. 1996;169:87-96.11. D. C. Hooper, et al., Prevention of Experimental Allergic Encephalomyelitis by Targeting Nitric Oxide and Peroxynitrite: Implications for the Treatment of Multiple Sclerosis. PNAS. 1997;94:2528-2533.12. S. Pfeiffer, et al., Interference of carboxy-PTIO with Nitric-Oxide and Peroxynitrite-Mediated Reactions. Free Radic Biol Med. 1997;22:787-794.

Dojindo细胞分析

细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐,称为WST。WST-8是高度稳定的WST,用于Cell Counting Kit-8(CCK-8)。由于WST-8甲maz是水溶性的,因此不会形成晶体。因此,不需要诸如MTT测定的增溶过程。此外,CCK-8的检测灵敏度高于其他四唑盐,例如MTT,XTT,MTS或WST-1。

WST检测机制

 

ß-半乳糖苷酶检测试剂

细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢

应用 产品展示
细胞生长检测,药物筛选,比色/荧光检测 细胞计数试剂盒-8
细胞计数试剂盒8 + 96孔有机硅定向剂
细胞计数试剂盒-F
细胞毒性LDH检测试剂盒
-WST 96孔有机硅定向剂
MTT
了解检测机制的差异: 点击这里
细胞周期分析 细胞周期测定溶液深红色
细胞周期测定溶液蓝色