Dojindo,Cellstain-AO溶液/1/A430,AO 与单链 DNA,RNA 形成复合物以发出红色荧光

2022年4月30日 作者 jinpanbio
吖啶橙 (AO) 与双链 DNA 形成复合物,发出绿色荧光(图 1)。 AO 还与单链 DNA 或 RNA 形成复合物以发出红色荧光。 一分子AO嵌入三对双链DNA碱基,发出绿色荧光,最大波长为526 nm(激发波长502 nm)。 一个 AO 分子还可以与单链 DNA 或 RNA 的一个磷酸基团相互作用,形成一个聚集或堆叠的结构,该结构发出最大波长为 650 nm(激发 460 nm)的红色荧光。 因此,AO 可用于检测双链 DNA 和单链 DNA 或 RNA。 它可以通过氩激光激发或流式细胞术同时测定 DNA 和 RNA。

Staining Procedure1.Prepare 10-50 μM AO solution with PBS or an appropriate buffer.a)2.Add AO solution with 1/10 of the volume of cell culture medium to the cell culture.b)3.Incubate the cell at 37ºC for 10-20 min.4.Wash cells twice with PBS or an appropriate buffer.5.Observe the cells under a fluorescence microscope with 500 nm excitation and 530 nm emission filters.

a) Since AO may be carcinogenic, extreme care is necessary during handling.b) You may replace the culture medium with 1/10 concentration of AO buffer solution.

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