Dojindo,EMCS/100/E018,杂双功能交联剂具有活化的酯和马来酰亚胺反应基团

2022年4月27日 作者 jinpanbio
杂双功能交联剂具有活化的酯和马来酰亚胺反应基团。 这些官能团分别与蛋白质的胺和巯基反应。 酶标记的半抗原是使用异双功能交联剂(例如 EMCS 或 GMBS)制备的。 交联反应需要中性 pH 值和温和的温度,因为在交联反应中需要保持酶活性和抗体滴度。 可使用具有 3、5、7 或 10 个线性碳链的异双功能交联剂。 这些线性脂肪链充当两个反应位点与其水溶性试剂之间的间隔物。 在更宽的 pH 范围内,它们比芳香族交联剂(例如 succinimidyl-4-N-maleimidobenzoate)更稳定。

Conjugation of Macromolecules with Hetero-Bifunctional Cross-Linking Reagent

Hetero-bifunctional Reagents

Product Name Code Length (Å) Solvent
EMCS E018 9.4 DMSO
GMBS G005 6.9 Chloroform, DMF
HMCS H257 13.0 Acetonitrile
KMUS K214 16.7 Acetonitrile, Methyl alcohol

 

1. S. Yoshitake, Y. Yamada, E. Ishikawa and R. Masseyeff, Conjugation of Glucose Oxidase from Aspergillus Niger and Rabbit Antibodies Using N-Hydroxysuccinimide Ester of N-(4-Carboxycyclohexylmethyl)-Maleimide, Eur. J. Biochem., 1979, 101, 395.2. T. Kitagawa, T. Shimozono, T. Aikawa, T. Yoshida and H. Nishimura, Preparation and Characterization of Hetero-bifunctional Cross-linking Reagents for Protein Modifications, Chem. Pharm. Bull., 1981, 29, 1130.3. S. Yoshitake, M. Imagawa, E. Ishikawa, Y. Niitsu, I. Urushizaki, M. Nishimura, R. Kanazawa, H. Kurosaki, S. Tachibana, N. Nakazawa and H. Ogawa, Mild and Efficient Conjugation of Rabbit Fab Eand Horseradish Peroxidase Using a Maleimide Compound and Its Use for Enzyme Immunoassay, J. Biochem., 1982, 92, 1413.4. E. Ishikawa, M. Imagawa, S. Hashida, S. Yoshitake, Y. Hamaguchi and T. Ueno, Enzyme-Labelling of Antibodies and Their Fragments for Enzyme Immunoassay and Immunohistochemical Staining, J. Immunol., 1983, 4, 209.5. I. Weeks, A. K. Campbell and J. S. Woodhead, Two-site Immunochemiluminometric Assay for Human α1-Fetoprotein, Clin. Chem., 1983, 29, 1480.6. S. Hashida, M. Imagawa, S. Inoue, K. Ruan and E. Ishikawa, More Useful Maleimide Compounds for the Conjugation of Fab Eto Horseradish Peroxidase through Thiol Groups in the Hinge, J. Appl. Biochem., 1984, 6, 56.7. S. Inoue, M. Imagawa, S. Hashida, K. Ruan and E. Ishikawa, A Small Scale Preparation of Affinity-Purified Rabbit Fab EHorseradish Peroxidase Conjugate for Enzyme Immunoassay, Anal. lett., 1984, 17, 229.8. M. Koizumi, K. Endo, M. Kunimatsu, H. Sakahara, T. Nakashima, Y. Kawamura, Y. Watanabe, T. Saga, J. Konishi, T. Yamamura, S. Hosoi, S. Toyama, Y. Arano and A. Yokoyama, Ga-Labeled Antibodies for Immunoscintigraphy and Evalution of Tumor Targeting of Drug-Antibody Conjugatesin Mice, Cancer Res., 1988, 48, 1189.9. T. Kohno and E. Ishikawa, Novel Enzyme Immunoassay(Immune Complex Transfer Enzyme Immunoassay) for Anti-insulin IgG in Guinea Pig Serum, Anal. lett., 1988, 21, 1019.