Dojindo,GMBS/100/G005,杂双功能交联剂具有活化的酯和马来酰亚胺反应基团
2022年4月27日杂双功能交联剂具有活化的酯和马来酰亚胺反应基团。 这些官能团分别与蛋白质的胺和巯基反应。 酶标记的半抗原是使用异双功能交联剂（例如 EMCS 或 GMBS）制备的。 交联反应需要中性 pH 值和温和的温度，因为在交联反应中需要保持酶活性和抗体滴度。 可使用具有 3、5、7 或 10 个线性碳链的异双功能交联剂。 这些线性脂肪链充当两个反应位点与其水溶性试剂之间的间隔物。 在更宽的 pH 范围内，它们比芳香族交联剂（例如 succinimidyl-4-N-maleimidobenzoate）更稳定。
Conjugation of Macromolecules with Hetero-Bifunctional Cross-Linking Reagent
Hetero-bifunctional Reagents
| Product Name | Code | Length (Å) | Solvent |
| EMCS | E018 | 9.4 | DMSO |
| GMBS | G005 | 6.9 | Chloroform, DMF |
| HMCS | H257 | 13.0 | Acetonitrile |
| KMUS | K214 | 16.7 | Acetonitrile, Methyl alcohol |
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