Dojindo,Cellstain钙黄绿素AM溶液/1/C396,Calcein-AM 与 Calcein 相比具有增强的疏水性
2022年4月26日Fig. 1 Cell staining mechanism
Staining Procedure1.Prepare 1 mM Calcein-AM solution with DMSO and dilute to prepare 1-50 μM Calcein-AM solution with PBS.a)2.Add Calcein-AM solution with 1/10 of the volume of cell culture medium to the cell culture.b)3.Incubate the cell at 37ºC for 15-30 min.4.Wash cells twice with PBS or an appropriate buffer.5.Observe the cells under a fluorescence microscope with 490 nm excitation and 515 nm emission filters.
a) If the Calcein-AM has difficulty loading into cells, use a detergent such as Pluronic F127.b) Or you may replace the culture medium with 1/10 concentration of Calcein-AM buffer solution.
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