Dojindo,抗硝基鸟苷多克隆抗体/50/AB01

2022年4月26日 作者 jinpanbio
8-Nitroguanosine 是 DNA 和 RNA 的硝化碱基。它是由一氧化氮和超氧阴离子自由基生成的过氧亚硝酸盐形成的。已知炎症产生的大量一氧化氮分子和超氧阴离子会引起鸟苷的硝化。由于化学修饰的核苷酸会在 DNA 复制过程中引起突变,因此 8-硝基鸟苷被认为是与突变和癌症相关的 DNA 损伤的标志之一。由于其非常高的特异性,单克隆抗体 NO2G52 可识别 8-硝基鸟嘌呤和 8-硝基鸟苷,但不与正常核苷酸碱基、8-羟基鸟嘌呤、8-羟基脱氧鸟苷、3-硝基酪氨酸、黄嘌呤或 2-硝基咪唑发生交叉反应。图。1)。 NO2G52 的特异性通过使用 8-硝基鸟苷-BSA 包被板的竞争性 ELISA 确定。如下图所示,NO2G52对8-硝基鸟嘌呤和8-硝基鸟苷具有非常高的亲和力,与8-溴鸟苷、8-溴鸟嘌呤和8-氯鸟嘌呤有轻微的交叉反应。抗硝基鸟苷多克隆抗体也可识别 8-硝基鸟苷和 8-硝基鸟苷,但它不与正常的鸟苷、鸟嘌呤、8-羟基鸟嘌呤或 3-硝基酪氨酸发生交叉反应。由于该抗体是使用兔制备的,因此可用于小鼠或大鼠等啮齿动物组织的免疫组织染色。

Specificity of Anti 8-Nitroguanosine monoclonal antibody

Tissue Staining with Anti-Nitroguanosine AntibodyA) Tissue section (8 days postinfection, influenza) stained with Anti-Nitroguanosine Antibody.B) The same section viewed using a confocal laser scanning microscope (Fluoroview FV300, Olympus, Nagano, Japan). Strong fluorescence, due to emission of Vector red, is evident in the cytosol.

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