Dojindo,生物膜活力检测试剂盒/B603,Biofilm Viability Assay Kit/100/B603
2022年4月26日Feature 1: Measurement time can be significantly shortened.In existing methods, biofilms are formed on the bottom of the well of a microplate, and additional work is required to change microbial culture media and wash sample several times before and after staining. However, our assay kits allow you to form biofilms on the pegs attached to the plate’s lid. Media change and staining process can be done simply by transferring the lid; therefore, our assay kits are very easy to use.
Feature 2: Our assay kits can reduce measurement variation.In existing methods, biofilm formation occurs on the bottom of the well of a microplate, which made biofilm easier to peel off because of the processes such as washing. This causes measurement variation, and this measurement variation was an issue for the quantification of biofilm. Our assay kits allow you to form biofilms on the pegs attached to the plate’s lid and make it possible to avoid peeling off biofilm which occurs during the process of the formation.
Two different types of assay kits for different purposes.We offer two types of assay kits that measure the amount of biofilm formation or the metabolic activities of live microorganisms within biofilms by using the same measurement method. You can select the one that best suits your needs.
Selection of 2 types of assay kits
*Conditions on biofilm formation vary according to microbial species and strains. When you need to examine these conditions, we first recommend you use the Biofilm Formation Assay Kit.*The Biofilm Formation Assay Kit is a product developed together with Fukuoka Industrial Technology Center.
Procedure
Example of measurementIndicators for drug-mediated inhibition of biofilm formation or antimicrobial effect of biofilms include MBIC (minimum biofilm inhibitory concentrations) and MBEC (minimum biofilm eradication concentrations). MBIC and MBEC of S. aureus were measured using each kit.
A3. In the Biofilm Formation Assay Kit (Product code: B601), please consider the amount of biofilm formation as a rough indication when a value of absorbance (590 nm) for crystal violet solution obtained after the final step is greater than 0.5.
A4. Dojindo Laboratories has evaluated the following microbial species that formed biofilms using the Biofilm Formation Assay Kit (Product code: B601) and the Biofilm Viability Assay Kit (this product).– Staphylococcus aureus– Pseudomonas aeruginosa– Escherichia coli– Streptococcus mutans– Porphyromonas gingivalisPlease refer to the technical manual, in terms of culture conditions for the above microbial species, experimental examples, etc.