Dojindo,谷氨酸检测试剂盒-WST/100/G269,Glutamate Assay-Kit-WST

PrincipleThe kit is used to detect Glutamate in cell culture medium by measuring the absorbance of WST formazan produced according to quantities of glutamate. The kit includes a “Glutamate Standard” which can be used to quantify the concentration of lactate found within samples by creating standard curve.

Simple Assay ProcedureProcedures are so easy that you simply incubate the plate after the addition of culture supernatant or tissue/cell lysates prior to adding a reagent.

Standard CurveStandard curve can be prepared by using Glutamate Standard included in the kit. The concentration of Glutamate can be measured. The concentration of lactate can be evaluated by diluting the samples if the concentration is over 0.5 mmol/l.

Example of Measurements of Glutamine/Glutamate level

Example of Measurements of Glutamine/Glutamate level in Ferroptosis researchIt is known that an iron-dependent cell death called “ferroptosis” is induced when xCT is inhibited by elastin treatment. The amounts of glutamate release and intracellular glutathione were measured by using the elastin-treated A549 cells. As a result, the amount of glutamate release was decreased in the elastin-treated A549 cells, and the amount of intracellular glutathione was decreased by inhibiting cystine uptake.