Dojindo,-Cellstain- CFSE/1/C375,CFSE 是细胞膜可渗透的

Staining Procedure1.Prepare 1 mM CFSE solution with DMSO. Dilute it to prepare 10-50 μM CFSE solution with PBS or an appropriate buffer.2.Add CFSE solution with 1/10 of the volume of cell culture medium to the cell culture.3.Incubate the cell at 37ºC for 15 to 30 min.4.Wash cells twice with PBS or an appropriate buffer.5.Observe the cells under a fluorescence microscope with 490 nm excitation and 530 nm emission filters.

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