biolegend流式抗体-Alexa Fluor® 700 anti-mouse CD45.2 Antibody

Pricing & Availability

Clone

104 (See other available formats)

Regulatory Status

RUO

Other Names

Ly-5.2, LCA

Isotype

Mouse (SJL) IgG2a, κ

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Product Citations

publications

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Compare all formats See Alexa Fluor® 700 spectral data

Technical data sheet

Product Details

Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

B10.S mouse thymocytes and splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC – Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer’s capability of exciting and detecting the fluorochrome.

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Excitation Laser

Red Laser (633 nm)

Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴, in vivo and in vitro blocking of B cell responses^1,2, and immunohistochemical staining of acetone-fixed frozen sections³. 

Application References

(PubMed link indicates BioLegend citation)

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2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed

Product Citations

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2. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
3. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
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12. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
13. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
14. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
15. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
16. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
17. Page N, et al. 2018. Immunity. 48:937. PubMed
18. Louveau A, et al. 2018. Nat Neurosci. 21:1380. PubMed
19. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
20. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
21. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
22. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
23. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
24. Nabet BY et al. 2017. Cell. 170(2):352-366 . PubMed
25. Ersching J et al. 2017. Immunity. 46(6):1045-1058 . PubMed
26. Benci JL et al. 2016. Cell. 167(6):1540-1554 . PubMed
27. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
28. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
29. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
30. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
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33. Ciecko AE, et al. 2019. Cell Rep. 29:3073. PubMed
34. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
35. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
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RRID

AB_493730 (BioLegend Cat. No. 109821)
AB_493731 (BioLegend Cat. No. 109822)

Antigen Details

Structure

Protein tyrosine phosphatase (PTP) family, 180-240 kD

Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function

Phosphatase, T and B cell activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers

Molecular Family

CD Molecules

Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID

19264 View all products for this Gene ID

UniProt

View information about CD45.2 on UniProt.org

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Related FAQs

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Other Formats

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