biolegend流式抗体-FITC anti-mouse CD45.2 Antibody

Pricing & Availability

Clone

104 (See other available formats)

Regulatory Status

RUO

Other Names

Ly-5.2, LCA

Isotype

Mouse (SJL) IgG2a, κ

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Product Citations

publications

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Technical data sheet

Product Details

Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

B10.S mouse thymocytes and splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC – Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)

Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴, in vivo and in vitro blocking of B cell responses^1,2, and immunohistochemical staining of acetone-fixed frozen sections³. 

Application References

(PubMed link indicates BioLegend citation)

1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed

Product Citations

1. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
2. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
3. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
4. Emgård J, et al. 2018. Immunity. 143:419. PubMed
5. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
6. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
7. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
8. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
9. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
10. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
11. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
12. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
13. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
14. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
15. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
16. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
17. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
18. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
19. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
20. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
21. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
22. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
23. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
24. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
25. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
26. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
27. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
28. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
29. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
30. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
31. Wan X, et al. 2018. Nature. 560:107. PubMed
32. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
33. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
34. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
35. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
36. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
37. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
38. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
39. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
40. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
41. Burman A, et al. 2007. Blood. 110:1064. PubMed
42. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
43. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
44. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
45. Burgess M, et al. 2014. Blood. 124:3947. PubMed
46. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
47. Koyama M, et al. 2015. J Exp Med. 212: 1303 – 1321. PubMed
48. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
49. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
50. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
51. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
52. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
53. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
54. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
55. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
56. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
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58. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
59. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
60. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
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RRID

AB_313442 (BioLegend Cat. No. 109805)
AB_313443 (BioLegend Cat. No. 109806)

Antigen Details

Structure

Protein tyrosine phosphatase (PTP) family, 180-240 kD

Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function

Phosphatase, T and B cell activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers

Molecular Family

CD Molecules

Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID

19264 View all products for this Gene ID

UniProt

View information about CD45.2 on UniProt.org

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Related FAQs

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Other Formats

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