Intercept® (PBS) Blocking Buffer,(PBS) 封闭缓冲液

Reagents > Intercept® (PBS) Blocking Buffer

Intercept® (PBS) Blocking Buffer

Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system.

  • Contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies.
  • Excellent choice for biotin-streptavidin detection.
  • Suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays.

For larger pack sizes, please contact your local sales office.

Intercept (PBS) 封闭缓冲液经过配制，可为可复制的定量蛋白质印迹和其他免疫测定提供高效封闭和低背景变异性。 当使用磷酸盐缓冲盐水 (PBS) 系统时，Intercept (PBS) 封闭缓冲液是近红外荧光应用的首选封闭缓冲液。

不含哺乳动物蛋白，与哺乳动物抗体的交叉反应性最低。
生物素-链霉亲和素检测的绝佳选择。
适用于广泛的应用，包括蛋白质印迹、In-Cell Western™ 检测和蛋白质阵列。
如需更大的包装尺寸，请联系您当地的销售办事处。

Formulation

Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon®-FL PVDF and nitrocellulose membranes.

Intercept (PBS) 封闭缓冲液是 PBS 中的即用型封闭剂配方。 它不含叠氮化钠，因此适用于近红外或化学发光检测。 它在 4 °C 下储存，也可用于包含 Immobilon®-FL PVDF 和硝酸纤维素膜的试剂盒中。

Shake well before each use.

Intercept® (PBS) Blocking Buffer
Figure 1: Western blot protocol demonstrating the comparison of Intercept Blocking Buffer to Odyssey Blocking Buffer. Blots with serial dilution of mouse heart tissue extract were probed for Stat3 Mouse mAb and p38 MAPK Rabbit Ab, detected with IRDye® 800CW Goat anti-Mouse IgG (P/N 925-32210) and IRDye 680LT Goat anti-Rabbit IgG (P/N 925-68021), and scanned together on an Odyssey CLx Imaging System.
图 1：Western 印迹协议，展示了 Intercept Blocking Buffer 与 Odyssey Blocking Buffer 的比较。 使用 IRDye® 800CW 山羊抗小鼠 IgG（部件号 925-32210）和 IRDye 680LT 山羊抗兔 IgG（部件号/ N 925-68021)，并在 Odyssey CLx 成像系统上一起扫描。

Intercept (PBS) Blocking Buffer can be used for many immunoassays and applications, including:

  • Quantitative Western Blot
  • Chemiluminescent Western Blots
  • In-Cell Western™ Assay
  • In-Gel Western
  • Protein Array
  • Glycoprotein Detection
  • 定量蛋白质印迹
    化学发光蛋白质印迹
    In-Cell Western™ 检测
    凝胶西部
    蛋白质阵列
    糖蛋白检测

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