CellTag® 用于 In-Cell Western™ 的 700 染色剂； 化验,CellTag™ 700 Stain for In-Cell Western™ Assays
2022年3月23日Reagents > CellTag™ 700 Stain for In-Cell Western™ Assays
CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.
The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.
CellTag 700 Stain is detected in the 700 nm channel of Odyssey® CLx, Classic, and Sa Imaging Systems. CellTag 700 Stain is applied to the cells during incubation with an IRDye® 800CW secondary antibody, and enables accurate measurement of target protein levels with much higher throughput than Western blotting.
CellTag 700 Stain 是一种近红外荧光非特异性细胞染料，可为 In-Cell Western™ Assay 应用提供准确的细胞数标准化。
染色剂在透化细胞的细胞核和细胞质中积累，并在广泛的细胞类型和细胞数量中提供线性荧光信号。
在 Odyssey® CLx、Classic 和 Sa 成像系统的 700 nm 通道中检测到 CellTag 700 Stain。 CellTag 700 Stain 在与 IRDye® 800CW 二抗孵育期间应用于细胞，可准确测量靶蛋白水平，其通量远高于蛋白质印迹法。
Properties
- Size: 2 x 10 nmole (lyophilized)
- λEx/λEm: 675/697 nm
- Recommended concentration: 0.2 μM
Linear Relationship Between Fluorescence and Cell Number
