LI-COR-试剂和耗材

CellVue® NIR815 荧光细胞标记试剂盒,CellVue® NIR815 Fluorescent Cell Labeling Kit

2022年3月23日 作者 jinpanbio

Reagents > CellVue® NIR815 Fluorescent Cell Labeling Kit

CellVue® NIR815 Fluorescent Cell Labeling Kit

The CellVue NIR815 cell labeling kit uses proprietary membrane labeling technology to stably incorporate CellVue NIR815, a fluorescent dye with long aliphatic tails, into lipid regions of the cell membrane1. The labeling buffer provided with the kit (Diluent C) is an iso-osmotic aqueous solution which contains no physiologic salts or buffers, detergents, or organic solvents and is designed to maintain cell viability while maximizing dye solubility and staining efficiency. The labeling efficiency of staining is dependent upon the cell type being labeled and the membranes of the cells2, 3.

CellVue NIR815 细胞标记试剂盒使用专有的膜标记技术将具有长脂肪族尾巴的荧光染料 CellVue NIR815 稳定地结合到细胞膜的脂质区域1。 试剂盒随附的标记缓冲液（稀释剂 C）是一种等渗水溶液，不含生理盐或缓冲液、去污剂或有机溶剂，旨在保持细胞活力，同时最大限度地提高染料溶解度和染色效率。 染色的标记效率取决于被标记的细胞类型和细胞膜2、3。

NIR Cell Linker Used for Short-term in vitro Studies

CellVue NIR815 dye has been reported to be useful for short-term in vitro cell proliferation studies4, cell tracking in isolated organ preparation applications5, and cell tracking using the Pearl® Small Animal Imaging System or Odyssey® CLx Imager or Odyssey Classic Infrared Imaging System6.

Kit Components

  • CellVue NIR815 dye stock (1 vial containing 0.1 mL, 1 x 10-3 M in ethanol)
  • Diluent C (1 vial containing 10 mL, sufficient for 5 labeling reactions)

Excitation and Emission Properties of CellVue NIR815 Dye

The excitation and emission properties of CellVue NIR815 dye are compatible with a range of commercially available near-infrared fluorescent plate readers, flow cytometers, in vitro and in vivo fluorescent imaging systems, and confocal microscopes.

CellVue NIR815 染料的激发和发射特性与一系列市售的近红外荧光读板机、流式细胞仪、体外和体内荧光成像系统以及共聚焦显微镜兼容。

CellVue® NIR815 Fluorescent Cell Labeling Kit
CellVue NIR815 Excitation and Emission Spectra (ex max = 786 nm;em max = 814nm).

Example Data

CellVue® NIR815 Fluorescent Cell Labeling Kit
Imaging of CellVue NIR815 in the capillary region of the lungs of a nude mouse. Image captured with Pearl immediately following intravenous injection into tail vein. Green: CellVue NIR815 cell stain. Red: autofluorescence in animal’s gut detected at 680 nm (caused by chlorophyll in the animal’s diet). Both fluorescent signals are overlaid on a white light image of the mouse.

References

  1. Horan, P. K., and Slezak, S. E., Nature, 340, 167-168 (1989).
  2. Horan, P. K., et al., Methods Cell Biol., 33, 469-490 (1990).
  3. Poon, R.Y., et al. In Living Color: Flow Cytometry and Cell Sorting Protocols, Diamond, R. A., and DeMaggio, S. (Eds.). p. 302-352 (Springer-Verlag, New York, 2000).
  4. Stewart, C. C., Woodring, M. L., Podniesinski, E. and Gray, B. D. Flow Cytometer in the Infrared: Inexpensive Modifications to a Commercial Instrument. Cytometry, Part A, 67A, #2, 104-111 (2005).
  5. Al-Mehdi, A-B, et al. Increased Depth of Cellular Imaging in the Intact Lung Using Far Red and Near Infrared Fluorescent Probes. Int. J. Biomed. Imag., Vol 2006, Article ID 37470, p 1-7.
  6. Thomas, D. L., et al., Experimental Manipulations of Afferent Immune Responses Influence Efferent Immune Responses to Brain Tumors. Cancer Immunol. Immunother.Sep, 57(9): 1323-33 (2008).

CellVue is a registered trademark of PTI Research, Inc, used under license. CellVue products are sold under license from PTI Research, Inc.. US Patent # 8,029,767B2.

标签CellVue® NIR815 Fluorescent Cell Labeling Kit CellVue® NIR815 荧光细胞标记试剂盒

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