LI-COR P/N 925-68030,P/N 926-68030,IRDye® 680LT 驴抗豚鼠 IgG 二抗,2D 凝胶检测,组织切片成像

2022年3月21日作者 jinpanbio

Reagents > IRDye^® 680LT Donkey anti-Guinea Pig IgG Secondary Antibody

Immunogen

Guinea Pig IgG, whole molecule

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG as well as the light chains common to other guinea pig immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins. The conjugate has been specifically tested and qualified for Western blot applications.

使用固定在琼脂糖珠上的抗原通过亲和层析实现特异性抗体的分离。基于免疫电泳和/或 ELISA，抗体与全分子豚鼠 IgG 以及其他豚鼠免疫球蛋白共有的轻链反应。未检测到针对非免疫球蛋白血清蛋白的反应性。该抗体已通过 ELISA 和/或固相吸附测试，以确保与牛、鸡、山羊、叙利亚仓鼠、马、人、小鼠、兔、大鼠和绵羊血清蛋白的交叉反应最小。该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

Recommended for:

Western Blot
Protein Array
Immunohistochemistry
Microscopy
2D Gel Detection
Tissue Section Imaging
蛋白质印迹
蛋白质阵列
免疫组织化学
显微镜
2D 凝胶检测
组织切片成像

Not Recommended for:

Small Animal Imaging
In-Cell Western Assay
On-Cell Western Assay
小动物成像
细胞内西方检测
On-Cell Western 检测

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 680LT 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。避光。在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。按照指示重新配制时，浓度为 1.0 mg/mL。有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application	Recommended	Suggested Range
Odyssey^® Western blot detection	1:20,000	1:20,000 – 1:50,000
Other	User optimized

Optimum dilutions will vary and should be determined empirically.

Note: When using PVDF membranes for Western blotting applications, SDS (final concentration of 0.01 – 0.02%) and Tween^® 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

注意：当使用 PVDF 膜进行蛋白质印迹应用时，必须在检测孵育步骤中添加 SDS（终浓度为 0.01 – 0.02%）和 Tween® 20（终浓度为 0.1 – 0.2%）以避免非特异性背景染色。

RRID

P/N 925-68030: RRID AB_2814910
P/N 926-68030: RRID AB_10706310

Example Data

标签2D 凝胶检测 IRDye® 680LT 驴抗豚鼠 IgG 二抗 LI-COR P/N 925-68030 P/N 926-68030 组织切片成像