LI-COR P/N 926-68051,IRDye® 680LT 山羊抗小鼠 IgG2a 特异性二抗

2022年3月21日作者 jinpanbio

Reagents > IRDye^® 680LT Goat anti-Mouse IgG_2a-Specific Secondary Antibody

Immunogen

Mouse IgG_2a paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography on mouse IgG_2a covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG_2a. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG₁, IgG_2b, IgG₃, and IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot. Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection.

通过对与琼脂糖共价连接的小鼠 IgG2a 进行亲和层析来分离特异性抗体。基于 ELISA 和流式细胞术，该抗体与小鼠 IgG2a 的重链发生反应。该抗体已通过斑点印迹和/或固相吸附测试，以确保与小鼠 IgM、IgG1、IgG2b、IgG3 和 IgA、合并的人血清和纯化的人副蛋白的交叉反应最小。该偶联物经过专门测试并符合蛋白质印迹的要求。一抗的结果可能会有所不同，在进行双色检测之前应确认特异性。

Applications

Recommended for:

Western Blot
Protein Array
Immunohistochemistry
Microscopy
2D Gel Detection
Tissue Section Imaging
蛋白质印迹
蛋白质阵列
免疫组织化学
显微镜
2D 凝胶检测
组织切片成像

Not Recommended for:

Small Animal Imaging
In-Cell Western Assay
On-Cell Western Assay

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 680LT 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。避光。在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。按照指示重新配制时，浓度为 1.0 mg/mL。有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application	Recommended	Suggested Range
Odyssey Western blot detection	1:20,000	1:20,000 – 1:50,000
Other	User optimized

Optimum dilutions will vary and should be determined empirically.

Note: When using PVDF membranes for Western blotting applications, SDS (final concentration of 0.01 – 0.02%) and Tween^® 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

注意：当使用 PVDF 膜进行蛋白质印迹应用时，必须在检测孵育步骤中添加 SDS（终浓度为 0.01 – 0.02%）和 Tween® 20（终浓度为 0.1 – 0.2%）以避免非特异性背景染色。

RRID

P/N 926-68051: RRID AB_2783643

标签IRDye® 680LT 山羊抗小鼠 IgG2a 特异性二抗 LI-COR P/N 926-68051