LI-COR IRDye® 800CW 蛋白质标记试剂盒,LI-COR IRDye 800CW

2022年3月19日 作者 jinpanbio

Reagents > IRDye® 800CW Protein Labeling Kits,IRDye® 800CW 蛋白质标记试剂盒

IRDye® 800CW Protein Labeling Kits

IRDye 800CW Protein Labeling Kits can be used to label antibodies and other proteins for applications such as Western blots, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays.

IRDye 800CW 蛋白质标记试剂盒可用于标记抗体和其他蛋白质,用于蛋白质印迹、In-Cell Western™ 测定、体内成像以及整个器官或组织切片测定等应用。

High Molecular Weight

Optimized for labeling 1.0 mg amounts of protein with molecular weight 45 – 200 kDa.

针对标记 1.0 mg 分子量 45 – 200 kDa 的蛋白质进行了优化。

This kit contains:

    • 3 x 0.1 mg IRDye 800CW Reactive Dye vials
    • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
    • 25 mL 1X PBS
    • 0.5 mL ultra-pure water
    • 3 x Pierce® Zeba™ Desalting Spin Columns (P/N 89891)

Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.

  • Pierce Zeba Desalting Spin column instructions
  • Protocol for IRDye 800CW Protein Labeling Kit – High MW

Low Molecular Weight

Optimized for labeling 1.0 mg of protein with molecular weight 14 – 45 kDa.

This kit contains:

    • 3 x 0.5 mg IRDye 800CW Reactive Dye vials
    • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
    • 25 mL 1X PBS
    • 0.5 mL ultra-pure water
    • 3 x Pierce Zeba Desalting Spin Columns (P/N 89891)

Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.

  • Pierce Zeba Desalting Spin column instructions
  • Protocol for IRDye 800CW Protein Labeling Kit – Low MW

Microscale

Optimized for labeling 100 μg of protein with molecular weight 14 – 200 kDa.

This kit contains:

    • 3 x 0.1 mg IRDye 800CW Reactive Dye vials
    • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
    • 25 mL 1X PBS
    • 0.5 mL ultra-pure water
    • 3 x Pierce Zeba Desalting Spin Columns (P/N 89892)

Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.

  • Pierce Zeba Desalting Spin Column instructions
  • Protocol for IRDye 800CW Protein Labeling Kit – Microscale