LI-COR 化学发光西部片的 WesternSure® ECL 剥离缓冲液

Reagents > WesternSure® ECL Stripping Buffer for Chemiluminescent Westerns,用于化学发光西部片的 WesternSure® ECL 剥离缓冲液

WesternSure® ECL Stripping Buffer for Chemiluminescent Westerns

WesternSure ECL Stripping Buffer is ideal for stripping and reprobing chemiluminescent Western blots. This Western blot stripping buffer:

  • Removes primary and secondary antibodies from PVDF or nitrocellulose membranes
  • Maintains target antigen integrity for efficient reprobing
  • Does not require hazardous shipping, unlike many other stripping buffers

Stripping buffers are not recommended if bands need to be quantified.

Note: WesternSure ECL Stripping Buffer is not recommended for loading amounts over 30 µg. It will demonstrate decreased performance when used at 2X or 5X concentration and heating the solution will not increase the stripping efficiency.

WesternSure ECL 剥离缓冲液是剥离和重新检测化学发光蛋白质印迹的理想选择。 这种蛋白质印迹剥离缓冲液：

从 PVDF 或硝酸纤维素膜上去除一抗和二抗
保持目标抗原的完整性，以实现高效的重新探测
与许多其他剥离缓冲液不同，不需要危险运输
如果需要量化条带，不建议使用剥离缓冲液。

注意：WesternSure ECL 剥离缓冲液不推荐用于超过 30 µg 的上样量。 当以 2X 或 5X 浓度使用时，它会表现出性能下降，并且加热溶液不会提高剥离效率。

Specifications

WesternSure ECL Stripping Buffer is provided as a 5X concentrated solution. It is stored at room temperature.

Data Example of Stripping and Reprobing with WesternSure ECL Stripping Buffer

WesternSure® ECL Stripping Buffer for Chemiluminescent Westerns
WesternSure ECL Stripping Buffer is effective for reprobing with different antibodies. Original: EGF-stimulated A431 lysate (10 and 5 µg total protein) was probed with mouse anti-ERK2 followed by detection with WesternSure HRP Goat anti-Mouse (P/N 926-80010) and WesternSure PREMIUM Chemiluminescent Substrate (P/N 926-95000). The blot was exposed to film for 20 sec. Stripped: The blot was then stripped with WesternSure ECL Stripping Buffer (P/N 926-92000), followed by detection with WesternSure PREMIUM, and exposed to film for 10 min. Reprobed: The blot was re-blocked with and reprobed with β-Actin Monoclonal (P/N 926-42212) followed by detection with HRP Goat anti-Mouse and WesternSure PREMIUM. The blot was exposed to film for 20 sec.
WesternSure ECL 剥离缓冲液可有效用于不同抗体的重新检测。 原文：EGF 刺激的 A431 裂解物（10 和 5 µg 总蛋白）用小鼠抗 ERK2 进行探测，然后用 WesternSure HRP 山羊抗小鼠（P/N 926-80010）和 WesternSure PREMIUM 化学发光底物（P/N 926）进行检测 -95000)。 将印迹暴露在胶片上 20 秒。 剥离：然后用 WesternSure ECL 剥离缓冲液 (P/N 926-92000) 剥离印迹，然后用 WesternSure PREMIUM 检测，并在胶片上曝光 10 分钟。 重新探测：用 β-肌动蛋白单克隆抗体 (P/N 926-42212) 重新封闭印迹并重新探测，然后用 HRP Goat anti-Mouse 和 WesternSure PREMIUM 进行检测。 将印迹暴露在胶片上 20 秒。

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