Labeled Streptavidins,标记的链霉亲和素,IRDye® 800CW 链霉亲和素

Streptavidin binds to biotin very strongly and is used to detect biotinylated proteins.

Fluorescent dye-labeled streptavidins can be used as a secondary detection reagent for microscopy, protein arrays, In-Gel Westerns, and Western blotting.

VRDye™ 490 链霉亲和素

链霉亲和素与生物素的结合非常强烈，可用于检测生物素化的蛋白质。

荧光染料标记的链霉亲和素可用作显微镜检查、蛋白质阵列、In-Gel Westerns 和蛋白质印迹的二级检测试剂。

IRDye and VRDye Streptavidin Specifications

All streptavidins are supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.

To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. For IRDye^® Streptavidin, a final concentration of 0.2 to 1.0 µg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications. For VRDye™ Streptavidin, a concentration of 1:2,000 to 1:5,000 is usually satisfactory for Western blotting. However, appropriate dilution may need to be determined empirically.

For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween^® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.

所有链霉亲和素均以缓冲液形式提供，缓冲液中含有 10 mM 磷酸盐、183 mM NaCl、2.7 nM KCl，pH 7.4，叠氮化钠 0.005% (w/v) 作为防腐剂。

要使用，请在使用前短暂离心以消除可能在溶液中形成的聚集体。 对于 IRDye® Streptavidin，0.2 至 1.0 µg/ml（1:1,000 至 1:5,000）的最终浓度通常对于大多数应用来说是令人满意的。 对于 VRDye™ Streptavidin，1:2,000 至 1:5,000 的浓度通常可以满足蛋白质印迹的要求。 但是，可能需要根据经验确定适当的稀释度。

对于基于膜的应用和 In-Gel Western，建议在检测孵育步骤中添加 SDS（0.02% 至 0.1% 最终浓度）和 Tween® 20（0.1% 至 0.2% 最终浓度）以减少非 特定背景染色。