LI-COR 的可见荧光染料产品,IRDye® 650 二抗,VRDye™ 549 二抗,VRDye™ 490 二抗
2022年3月22日
IRDye® 650 Secondary Antibodies,IRDye® 650 二抗
VRDye™ 549 Secondary Antibodies,VRDye™ 549 二抗
VRDye™ 490 Secondary Antibodies,VRDye™ 490 二抗
Use Visible Fluorescent Dye Conjugates for Microscopy and Flow Cytometry
LI-COR’s visible fluorescent dye products include labeled secondary antibodies and labeling kits.
VRDye™ 549, VRDye 490, and IRDye® 650 secondary antibodies are labeled with visible fluorescent dyes and are optimized for use in Western blotting, microscopy, immunohistochemistry, and flow cytometry applications when imaged on instruments with suitable excitation and emission filters.
These secondary antibodies, like LI-COR IRDye infrared fluorescent secondary antibodies, are highly cross-adsorbed.
The visible fluorescent protein labeling kits are ideal if you need to label your custom monoclonal antibodies for flow cytometry.
LI-COR 的可见荧光染料产品包括标记的二抗和标记试剂盒。
VRDye™ 549、VRDye 490 和 IRDye® 650 二抗用可见荧光染料标记，并经过优化，可在具有合适激发和发射滤光片的仪器上成像时用于蛋白质印迹、显微镜检查、免疫组织化学和流式细胞术应用。
这些二抗，如 LI-COR IRDye 红外荧光二抗，具有高度交叉吸附性。
如果您需要为流式细胞术标记定制的单克隆抗体，可见荧光蛋白标记试剂盒是理想的选择。
Properties of Visible Fluorescence NHS Ester Dyes
| Dye | Absmax | Emmax | ε (M-1cm-1) | MW (g/mole) | CF* |
|---|---|---|---|---|---|
| IRDye 650 | 648 | 655 | 230,000 | 1,097 | 0.03 |
| VRDye 549 | 550 | 575 | 150,000 | 1,040 | 0.07 |
| VRDye 490 | 491 | 515 | 73,000 | 1,011 | 0.11 |
*CF is a correction factor for the absorbance of the dye at Absmax to the absorbance of the protein at 280 nm.