蛋白质印迹试剂盒,带有 PVDF 膜的蛋白质印迹试剂盒,带有硝酸纤维素膜的蛋白质印迹试剂盒

Western Blotting Kits

Western Blotting Kits with PVDF Membranes,带有 PVDF 膜的蛋白质印迹试剂盒

Western Blotting Kits

Western Blotting Kits with Nitrocellulose Membranes,带有硝酸纤维素膜的蛋白质印迹试剂盒

Western Blotting Kits can be used for quantitative Western blots or RNAi studies in which a phosphate-buffered saline (PBS) buffering system or a Tris-buffered saline (TBS) is used.

Convenient Kits for Multiplex Western Blots

These sample kits allow you to obtain a smaller quantity of infrared reagents and corresponding membranes to perform quantitative Western blots without committing to a large quantity of each.

  • Conveniently test LI-COR reagents and membranes for your Western blotting
  • Use near-infrared fluorescence detection on the Odyssey® Imaging systems
  • Experiment with multiplex Western blotting for simultaneous two-color detection
  • 这些样品试剂盒允许您获得较少量的红外试剂和相应的膜来进行定量蛋白质印迹,而无需大量使用。

    方便地测试用于蛋白质印迹的 LI-COR 试剂和膜
    在 Odyssey® 成像系统上使用近红外荧光检测
    使用多重蛋白质印迹实验同时进行双色检测

Kit Components

Each kit contains one IRDye® 800CW Dye-Labeled Secondary Antibody for the 800nm channel, plus an additional secondary antibody labeled with IRDye 680RD for the 700nm channel. Kits are available with either 10 Immobilon®-FL PVDF Membranes (0.45 μm, 10 cm x 10 cm) or 10 Odyssey Nitrocellulose Membranes (0.22 μm, 7 cm x 8.5 cm). All kits include Intercept™ (TBS) Blocking Buffer or Intercept (PBS) Blocking Buffer.

每个试剂盒包含一个用于 800nm 通道的 IRDye® 800CW 染料标记二抗,以及一个用于 700nm 通道的用 IRDye 680RD 标记的附加二抗。 套件提供 10 个 Immobilon®-FL PVDF 膜(0.45 μm,10 cm x 10 cm)或 10 个 Odyssey 硝酸纤维素膜(0.22 μm,7 cm x 8.5 cm)。 所有试剂盒均包括 Intercept™ (TBS) 封闭缓冲液或 Intercept (PBS) 封闭缓冲液。

Use Western Blotting Kits for Multiplexing

Western Blotting Kits
Dose response of EGFR phosphorylation in A431 cells. A431 cells were treated with serial dilutions of EGF (6.3-200 ng/mL). Lysates were resolved by SDS-PAGE and transferred to nitrocellulose. The blot was probed with rabbit anti-phosphoEGFR and mouse anti-ERK2 followed by detection with IRDye 800CW Goat anti-Rabbit IgG (P/N 926-32211) and IRDye 680RD Goat anti-Mouse IgG (P/N 926-68070).

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