The NEBNext^® Ultra^™ II Directional RNA Second Strand Synthesis Module has been optimized to

generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext Directional RNA library

preparation workflow.  This workflow uses the “dUTP” method for strand-specific library construction, which

depends on incorporation of uracil into the second strand cDNA, enabled by the NEBNext Second Strand

Synthesis Reaction Buffer with dUTP Mix, included in this module.

The NEBNext Ultra II Directional RNA Second Strand Synthesis Module is Designed for Use with the

Following:

 –  NEBNext^® Ultra^™ II RNA First Strand Synthesis Module (NEB #E7771)

 –  NEBNext^® Ultra^™ II End Repair/dA-Tailing Module (NEB #E7546)

 –  NEBNext^® Ultra^™ II Ligation Module (NEB #E7595)

 –  NEBNext^® Ultra^™ II Q5^® Master Mix (NEB #M0544)

 –  NEBNext^® Oligos for Illumina^®(NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600,         

    #E7535, #E7350)

NEBNext Second Strand Synthesis Enzyme Mix

NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix

 -20°C