biolegend流式抗体-Purified anti-human CD64 Antibody

Pricing & Availability

Clone

10.1 (See other available formats)

Regulatory Status

RUO

Workshop

VI MA36

Other Names

FcγRI, FcR I

Isotype

Mouse IgG1, κ

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Product Citations

publications

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Technical data sheet

Product Details

Reactivity

Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC – Quality tested
IHC-F, Block – Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis⁶ cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI^2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections¹².

Application References

(PubMed link indicates BioLegend citation)

1. McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
3. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
4. Holl V, et al. 2004. J. Immunol. 173:6274.
5. Hober D, et al. 2002. J. Gen. Virol. 83:2169.
6. Cho HJ, et al. 2007. Physiol Genomics 149:60.
7. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
8. Bruhns P, et al. 2008. Blood 113:3716. PubMed
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
10. Carter DL, et al. 1999. Cytometry 37:41. (FC)
11. Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
12. Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)

Product Citations

1. Prodjinotho UF, et al. 2017. PLoS Negl Trop Dis. 11:e0005777. PubMed
2. Chen W, Pilling D, and Gomer R 2017. BMC Immunol. . 10.1186/s12865-017-0230-z. PubMed
3. Elias S, et al. 2018. Oncoimmunology. 9:416. PubMed
4. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
5. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
6. Olin A, et al. 2018. Cell. 174:1277. PubMed
7. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
8. Chudnovskiy A et al. 2016. Cell. 167(2):444-456 . PubMed
9. Zhong Q, et al. 2018. J Immunol. 200:3913. PubMed
10. Vermi W, et al. 2018. Cancer Res. 78:3544. PubMed
11. Keskin DB, et al. 2019. Nature. 565:234. PubMed
12. Steffen U, et al. 2020. Nat Commun. 0.541666667. PubMed
13. Jog NR, et al. 2018. Front Immunol. 9:2198. PubMed
14. Rodriguez L, et al. 2020. Cell Reports Medicine. 1(5):100078. PubMed
15. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol . 25:717. PubMed
16. Cho H, et al. 2007. Physiol Genomics. 29:149. PubMed
17. Bruhns P, et al. 2008. Blood. 101182. PubMed
18. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
19. Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed
20. Henrick BM, et al. 2021. Cell. . PubMed

RRID

AB_314486 (BioLegend Cat. No. 305002)

Antigen Details

Structure

Ig superfamily, type I glycoprotein, 72 kD

Distribution

Monocytes, macrophages, dendritic cells, activated granulocytes

Function

Phagocytosis, ADCC

Ligand/Receptor

IgG receptor

Cell Type

Dendritic cells, Granulocytes, Macrophages, Monocytes

Biology Area

Immunology, Innate Immunity

Molecular Family

CD Molecules, Fc Receptors

Antigen References

1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.

Gene ID

2209 View all products for this Gene ID

UniProt

View information about CD64 on UniProt.org

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Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

Other Formats

View All CD64 Reagents Request Custom Conjugation