LI-COR IRDye® 800CW,IRDye® 800CW 叠氮化物红外染料

Reagents > IRDye® 800CW Azide Infrared Dye,IRDye® 800CW 叠氮化物红外染料

IRDye® 800CW Azide Infrared Dye

IRDye 800CW Azide provides the functionality for preferential labeling of molecules that contain either the alkyne or dibenzocyclooctyne (DBCO) group.

IRDye 800CW 叠氮化物提供优先标记含有炔烃或二苯并环辛炔 (DBCO) 基团的分子的功能。

Learn more about Near-Infrared Fluorescent Click Chemistry Reagents.

Dye Structure

IRDye® 800CW Azide Infrared Dye

Properties

  • Chemical Formula: C54H67N6Na3O17S4
  • Molecular Weight: 1269.36 g/mol
  • Exact Mass: 1268.31
  • Correction factor at 260 – 280 nm: 3%

Absorption and Emission Spectra

Solvent Absorption Maxima Emission Maxima
Methanol 778 nm 794 nm
1X PBS 774 nm 789 nm
1:1 Methanol to 1X PBS 778 nm

Absorbance and Emission Spectra in 1X PBS

IRDye® 800CW Azide Infrared Dye

Molar Absorptivity

Solvent Molar Absorptivity (L mol-1 cm-1)
Methanol 300,000
1X PBS 240,000
1:1 Methanol to 1X PBS 270,000

Example Data

IRDye® 800CW Azide Infrared Dye
Palmitoylation of Lck kinase analyzed with a Click Chemistry IRDye reagent.​​ Lck was immunoprecipitated from metabolically-labeled Jurkat cell lysates. Palmitoylation was detected with CuAAC click chemistry and IRDye 800CW Azide Infrared Dye​ (green signals; P/N 929-60000). Total Lck was detected with anti-Lck D88 antibody and IRDye 680RD Donkey anti-Rabbit IgG​ (red; P/N 926-68073). Yellow indicates overlap of signals. The mobility shift caused by palmitoylation is observed in lanes 3-5 and 7-9 (green bands migrating more slowly than red bands). Data and image supplied by a LI-COR customer.
使用 Click Chemistry IRDye 试剂分析 Lck 激酶的棕榈酰化。Lck 从代谢标记的 Jurkat 细胞裂解物中进行免疫沉淀。 使用 CuAAC 点击化学和 IRDye 800CW 叠氮化物红外染料（绿色信号；P/N 929-60000）检测棕榈酰化。 使用抗 Lck D88 抗体和 IRDye 680RD 驴抗兔 IgG（红色；P/N 926-68073）检测总 Lck。 黄色表示信号重叠。 在泳道 3-5 和 7-9 中观察到由棕榈酰化引起的迁移率变化（绿色条带迁移速度比红色条带慢）。 LI-COR 客户提供的数据和图像。

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