Dojindo,Ab-10 Rapid Peroxidase Labeling Kit/3/LK33,Ab-10 快速过氧化物酶标记试剂盒

IgG Labeling Reaction of NH2-reactive Peroxidase

Precaution♦ Use 0.5-1 mg/ml of antibody solution for labeling. If the antibody concentration is higher than 1mg/ml, dilute the antibody solution with PBS.♦ If the sample solution contains small insoluble materials, centrifuge the solution, and use the supernatant for the labeling.♦ The microtube in this kit contain solutions. Since there is a possibility that the droplets might be attached to the inside walls or caps, please centrifuge to drop the droplets prior to opening.♦ Some additives in antibody solution may interfere with the labeling if the concentration is too high. The maximum compatible concentrations of such additives are indicated on Table 1.♦ After a Reactive Fluorescent is removed from the seal bag, keep the unused Reactive Fluorescein in the bag, seal tightly and store at -20ºC.Store the other components at 0-5ºC.♦ Since reactive reagent binds to an amino group in antibody, there is a possibility that the labeled antibody loses the antigen recognition ability (Table 2).

Table. 1 Compatible concentrations of the additives

Table. 2 Non-Compatible Antibody

Fig. 3 Microscope Image of Mitochondria in Hela CellsPeroxidase were to anti-mitochondria antibody using Ab-10 Rapid Peroxidase Labeling Kit (code：LK33)