Dojindo,Cell Cycle Assay Solution Blue/250/C549,细胞周期的控制系统与细胞增殖密切相关

Simple ProcedurePropidium iodide (PI) is generally used for cell cycle analysis using flow cytometry. Compared to this analysis method, the Cell Cycle Assay Solution Blue has good membrane permeability and uses the dye with high specificity for DNA, which makes it possible to analyze the cell cycle just by adding the reagent to a cell suspension. Additionally, a 405 nm laser is available in the Cell Cycle Assay Solution Blue, allowing you to concurrently use a highly versatile 488 nm laser.

Clearly Identify Cell Cycle StagesLive CHO cells stained by the Cell Cycle Assay Solution Blue and Deep Red were measured by flow cytometry. Similar experiments were performed using the existing reagent for cell cycle analysis and PI staining a widely used staining technique. As a result, results obtained by the Cell Cycle Assay Solution were equivalent to PI staining results (shown below). Compared to four different products, our product obtained a sharp histogram peak in live cells.

Measurement ExampleDoxorubicin (DOX) acts to inhibit cell proliferation during G2/M phases of the cell cycle and induces cellular senescence. After adding DOX to A549 cells, higher histogram peaks for the G2/M phase (Cell Cycle Assay Solution Blue and Deep Red), induced cellular senescence (Cellular Senescence Detection Kit – SPiDER-βGal), and differences in mitochondrial membrane potential (JC-1 MitoMP Detection Kit) were observed compared to before.