Dojindo,细胞毒性 LDH 检测试剂盒-WST/2000/CK12

Choose between Two ProceduresCytotoxicity LDH Assay Kit-WST can be applied with and without supernatant transferring. Please choose suitable method for your experiment.

Assay Procedure[Optimization of cell concentration][Homogeneous Assay][Non-homogeneous Assay]

Stable Working SolutionWorking Solution is stable for 6 months under refrigerated conditions. Therefore, after the preparation, Working Solution can be used as a ready-to-use solution at any time during this period.

Experimental Examples

为了在细胞毒性测定中获得准确的结果，样品通过不同的原理进行测量。 Cell Counting Kit-8（产品代码：CK04）测量活细胞中的 NADH，LDH 测定法测量死细胞中释放的 LDH。 根据这些结果，随着毒物浓度的增加，活细胞减少，死细胞增加。

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Stability of Working Solution

Reconstituted Working Solution has higher stability than competitor’s kit, eliminating the additional time to prepare the Working Solution for each assay.

Comparison of Working Solution Stability

Linearity

Dojindo’s Cytotoxicity LDH Assay Kit-WST has higher sensitivity than competitor’s kits

Cytotoxicity of mitomycin C using HeLa cells

Culture medium: MEM, 10% FBSIncubation: 37C, 5% CO2, 48 hours