lonza,lonza CC-2581,CC-2512,CC-2527,PASMC-人肺动脉平滑肌细胞
2022年4月9日Cryopreserved ampule of Pulmonary Artery Smooth Muscle Cells (PASMC) containing ≥ 500,000 cells
Product Overview
Human Pulmonary Artery Smooth Muscle Cells (PASMC) are shipped in 3rd passage.
Human Pulmonary Artery Smooth Muscle Cells are guaranteed for 15 population doublings and to stain positive for alpha smooth muscle actin and negative for von Willebrand Factor VIII after differentiation.
All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
人肺动脉平滑肌细胞 (PASMC) 在第 3 代运输。
人肺动脉平滑肌细胞保证 15 次群体倍增，分化后 α 平滑肌肌动蛋白染色呈阳性，von Willebrand 因子 VIII 染色呈阴性。
所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。
Applications
- Stroke
- Diabetes
- Vascular research
- Artherosclerosis
- Hypertension
Content & Storage
Content
1 x Cryopreserved ampule PASMC containing ≥500,000 cells/vial
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PASMC – Human Pulmonary Artery Smooth Muscle Cells
Human pulmonary artery smooth muscle cells(CC-2581) were stained with 1:1500 dilution of Anti Alpha-Smooth Muscle Actin dilution of (CBL171, Sigma). Immunofluorescence analysis was conducted using Secondary donkey polyclonal antibody Alexa Fluor Do anti Mo 594 Invitrogen (Thermo Fisher) A21203 at a 1:200 dilution. Nuclei were stained with Hoechst . In multi-well culture, PAMCSs appeared typically epitheloid (polygonal) forming monolayers of very low-level alpha-actin expressing cells. Rarely, a few intensely alpha-actin positive cells (arrows) were evident. These displayed distinct fusiform-elongated morphology.
PASMC – Human Pulmonary Artery Smooth Muscle Cells
Cells are isolated from human pulmonary artery and expanded prior to freezing (CC-2581). When plated & expanded, the cells express little to no alpha-actin unless first stimulated to differentiate.Cells were stained with Anti Alpha-Smooth Muscle Actin Chemicon (Millipore) CBL171 at 1:1500 dilution. Immunofluorescence analysis was conducted using Secondary donkey polyclonal antibody Alexa Fluor Do anti Mo 594 Invitrogen (Thermo Fisher) A21203 at 1:200 dilution. Nuclei were stained with Hoechst.
