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Product Overview

Monocytes are found in the circulating peripheral blood where they make up 10-20% of the total mononuclear cell population. Monocytes are characterized by their phenotypic expression of CD14, and thus, are commonly called CD14+ monocytes. They play an important role in host defense as circulating monocytes and differentiation into tissue macrophages, and can differentiate into dendritic cells with potent antigen-presenting capability.

单核细胞存在于循环外周血中，占总单核细胞群的 10-20%。 单核细胞的特征在于它们的 CD14 表型表达，因此，通常称为 CD14+ 单核细胞。 它们作为循环单核细胞和分化成组织巨噬细胞在宿主防御中发挥重要作用，并且可以分化成具有强大抗原呈递能力的树突状细胞。

Cells You Can Count on to Perform

Lonza Human Peripheral Blood CD14+ Monocytes are isolated from peripheral blood of screened, healthy, untreated (non-mobilized peripheral blood) donors. Briefly, mononuclear cells (MNCs) are isolated from apheresis derived peripheral blood. The MNC population is then further purified via positive immunomagnetic selection directed against CD14. Cryopreserved CD14+ Monocytes are guaranteed to express CD14 and will contain either ≥10, 20, or 40 million viable cells per ampoule depending upon the product ordered. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Lonza Human Peripheral Blood CD14+ 单核细胞是从经过筛选的、健康的、未经治疗的（未动员的外周血）供体的外周血中分离出来的。 简而言之，单核细胞 (MNC) 是从单采术衍生的外周血中分离出来的。 然后通过针对 CD14 的阳性免疫磁性选择进一步纯化 MNC 群体。 冷冻保存的 CD14+ 单核细胞保证表达 CD14，并且根据订购的产品，每个安瓿将包含 ≥10、20 或 4000 万个活细胞。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Choices in Cell Type and Tissue Source

Hematopoietic and immune research requires a variety of donors and cell types. That’s why Lonza offers many different peripheral blood cell types from a variety of donors, including PBMCs and dendritic cells, among others. In addition, if you are interested in exploring the differences between monocytes in different maturation states or from different tissues, we offer bone marrow mononuclear cells.

造血和免疫研究需要多种供体和细胞类型。 这就是为什么 Lonza 提供来自各种供体的多种不同外周血细胞类型，包括 PBMC 和树突状细胞等。 此外，如果您有兴趣探索不同成熟状态或来自不同组织的单核细胞之间的差异，我们提供骨髓单核细胞。

Supported by Lonza Media

The recommended medium varies by application (see below). Please contact Scientific Support for additional culturing recommendations. Please note, media listed for macrophage and dendritic cell differentiation are recommendations only.

• Monocyte Phenotype: RPMI + 10% Fetal Bovine Serum + 2 mM L-glutamine + 10 ng/ml rHU M-CSF (optional)
• Macrophages: RPMI +10% Fetal Bovine Serum + 2 mM L-glutamine + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids + 25 ng/ml rHU M-CSF
• Dendritic Cells: LGM-3^TM Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4

Lonza CD14+ monocytes are not routinely quality control tested for macrophage or dendritic cell differentiation and differentiation is not guaranteed under the cell warranty. Additional factors including extracellular matrixes or culturing conditions may be required to produce differentiation.

Lonza guarantees the performance of Clonetics^TM/Poietics^TM cells only if appropriate Clonetics^TM/Poietics^TM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

Lonza CD14+ 单核细胞没有针对巨噬细胞或树突状细胞分化进行常规质量控制测试，并且细胞保修不保证分化。 产生分化可能需要其他因素，包括细胞外基质或培养条件。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。 如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。