LI-COR IRDye® 680RD BoneTag™ 光学探头,LI-COR IRDye 680RD 近红外染料
2022年3月18日Reagents > IRDye® 680RD BoneTag™ Optical Probe
IRDye 680RD BoneTag Optical Probe is a calcium-chelating compound conjugated to IRDye 680RD near-infrared dye. Using IRDye NIR dyes extends fluorescence signal detection to the near-infrared fluorescent region of the spectrum without affecting the compound’s ability to function as a marker of the mineralization process. NIR fluorescence detection improves depth of penetration due to low tissue autofluorescence, translating to low background interference.
Calcium-chelating compounds have been used effectively for the detection of bone mineralization, growth, and morphological changes, including tetracycline derivatives, xylenol orange, alizarin, calcein, and fluorescein. NIR-labeled pamidronate has also been successfully used for detection of arterial calcification in non-invasive optical imaging1.
IRDye 680RD BoneTag 光学探针是一种与 IRDye 680RD 近红外染料结合的钙螯合化合物。 使用 IRDye NIR 染料将荧光信号检测扩展到光谱的近红外荧光区域，而不影响化合物作为矿化过程标记的能力。 NIR 荧光检测由于组织自发荧光低而提高了穿透深度，从而转化为低背景干扰。
钙螯合化合物已被有效地用于检测骨矿化、生长和形态变化，包括四环素衍生物、二甲酚橙、茜素、钙黄绿素和荧光素。 NIR 标记的帕米膦酸盐也已成功用于检测无创光学成像中的动脉钙化1。
Learn about other LI-COR optical probes.
Applications
- Microscopy
- Bone Imaging
Absorbance and Emission Spectra
Data Example
References
- Zaheer A et al. (2001) In vivo near-infrared fluorescence imaging of osteoblastic activity. Nat Biotechnol, 19. 1148-54.
- Kovar, J. L., Xu, X., Draney, D., Cupp, A., Simpson, M. A., & Michael Olive, D. (2011). Anal Biochem, 416(2), 167–173. doi: 10.1016/j.ab.2011.05.011.