LI-COR 蛋白质印迹标准化Revert™ 520 总蛋白染色剂

2022年3月16日 作者 jinpanbio

Reagents > Revert™ 520 Total Protein Stain for Western Blot Normalization,用于蛋白质印迹标准化的 Revert™ 520 总蛋白染色剂

Revert™  520 Total Protein Stain for Western Blot Normalization

Make Western blot normalization more accurate and reliable with Revert 520 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 520 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment.

Revert 520 Total Protein Stain is a robust and replicable solution for Western blot normalization. The stain can be detected in the 520 nm channel for quantification and normalization analyses. The protocol is quick and can be completed within 15 minutes.

The 200 mL size is sufficient for 20 mini Western blots (5 mL/blot), and the 500 mL size is sufficient for 50 mini Western blots.

使用 Revert 520 Total Protein Stain 使蛋白质印迹标准化更加准确和可靠,这是一种基于膜的(转移后)标准化策略,可对样品中的所有蛋白质进行染色。 Revert 520 Total Protein Stain 适用于您现有的蛋白质印迹工作流程,不需要特殊的凝胶或设备。

Revert 520 Total Protein Stain 是一种用于蛋白质印迹标准化的强大且可复制的解决方案。 可以在 520 nm 通道中检测到染色,以进行量化和标准化分析。 该协议很快,可在 15 分钟内完成。

200 mL 的容量足以进行 20 次小型蛋白质印迹(5 毫升/印迹),500 毫升的容量足以进行 50 次小型蛋白质印迹。

Accurate, Reliable Western Blot Normalization with Revert 520 Stain

Revert 520 Total Protein Stain works with your existing Western blot workflow, without needing any additional reagents or special gels. Staining takes less than ten minutes.

Revert 520 Total Protein Stain 可与您现有的蛋白质印迹工作流程配合使用,无需任何额外试剂或特殊凝胶。 染色时间不到十分钟。

Revert™  520 Total Protein Stain for Western Blot Normalization
Figure1. Rapid staining with Revert 520 Total Protein Stain. Revert 520 Total Protein Stain provides highly efficient protein staining on nitrocellulose or Immobilon® FL PVDF membranes in under 10 minutes. A dilution series using 20 µg to 313 ng of mouse heart tissue lysate was separated in 4-12% NuPAGE Bis-Tris gels, transferred to nitrocellulose or PVDF membranes, and then imaged using the 520 nm channel of an Odyssey M Imager.
图1。 使用 Revert 520 Total Protein Stain 进行快速染色。 Revert 520 Total Protein Stain 可在 10 分钟内对硝酸纤维素膜或 Immobilon® FL PVDF 膜进行高效蛋白质染色。 使用 20 µg 至 313 ng 小鼠心脏组织裂解物的稀释系列在 4-12% NuPAGE Bis-Tris 凝胶中分离,转移到硝酸纤维素或 PVDF 膜上,然后使用 Odyssey M Imager 的 520 nm 通道成像。
Revert™  520 Total Protein Stain for Western Blot Normalization
Figure 2. Absorbance and emission spectra of the working solution for Revert 520 Total Protein Stain.
图 2. Revert 520 Total Protein Stain 工作溶液的吸光度和发射光谱。