LI-COR 标准化的 α-微管蛋白小鼠单克隆抗体,LI-COR P/N 926-42213
2022年3月15日Reagents > α-Tubulin Mouse Monoclonal Antibody for Normalization,用于标准化的 α-微管蛋白小鼠单克隆抗体
α-Tubulin primary antibody can be used as an internal loading control (ILC) for normalization.
The expression of α-tubulin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, α-tubulin primary antibodies can be used for the detection of α-tubulin when performing two-color detection.
Detect α-Tubulin Mouse Monoclonal Antibody with IRDye® Goat anti-Mouse or IRDye Donkey anti-Mouse secondary antibodies.
Other options for housekeeping protein normalization
α-微管蛋白一抗可用作内部上样对照 (ILC) 以进行标准化。
应验证 α-微管蛋白或任何管家蛋白 (HKP) 的表达，以确保其表达在实验条件下不会改变。
经验证后，α-微管蛋白一抗可在进行双色检测时用于检测 α-微管蛋白。
使用 IRDye® Goat anti-Mouse 或 IRDye Donkey anti-Mouse 二抗检测 α-Tubulin Mouse Monoclonal Antibody。
管家蛋白质标准化的其他选择
Reactivity and Specificity
α-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.Do not aliquot the antibody.
α-微管蛋白抗体以 10 mM HEPES (pH 7.5)、150 mM NaCl、100 µg/mL BSA、50% 甘油和 <0.02% 叠氮化钠的形式提供。
不要等分抗体。
| Properties | α-Tubulin Mouse Monoclonal Antibody (P/N 926-42213) |
|---|---|
| Species Cross-Reactivity | Human, mouse, rat, monkey |
| Target Molecular Weight | 52 kDa |
| Isotype | Mouse IgG1 |
| Specificity/Sensitivity | Detects endogenous levels of total α-tubulin protein. α/β-tubulin heterodimers form the tubulin subunit common to all eukaryotic cells.
检测总 α-微管蛋白的内源水平。 α/β-微管蛋白异二聚体形成所有真核细胞共有的微管蛋白亚基。 |
| Immunogen | Full-length chicken α-tubulin (purified from brain extracts) |
| Tested Applications | Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)
蛋白质印迹 (WB)、免疫组织化学 (IHC)、免疫荧光 (IF)、流式细胞术 (F) |
RRID
- P/N 926-42213: RRID AB_2637092