标签：原代细胞和干细胞

lonza,CC-2703,原代细胞和干细胞,人外周血单个核细胞(hPBMC) CC-2703

Catalog #: CC-2703

Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 100 million cells

Product Overview

Mononuclear cell (MNC) rich cells from leukapheresis are depleted of RBCs and platelets. MNC pools contain, on average, 50% T cells, 20% monocytes, 10% B cells, 10% NK cells and may contain up to 5% granulocytes. Cells are cryopreserved in Lonza cryopreservation media. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols.

来自白细胞分离术的富含单核细胞 (MNC) 的细胞耗尽了红细胞和血小板。 MNC 池平均含有 50% 的 T 细胞、20% 的单核细胞、10% 的 B 细胞、10% 的 NK 细胞，并且可能含有高达 5% 的粒细胞。 细胞在 Lonza 冷冻保存培养基中冷冻保存。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis (CoA) is provided for each cell lot purchased

Applications

  • ELISPOT
  • Proliferation assays
  • Immunogenicity assays
  • Cell therapy process development
  • Humanized mice
  • Biomarker discovery

Content & Storage

Content

1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 100 million cells

Instructions, SDS, CoA

lonza,原代细胞和干细胞,人外周血单个核细胞hPBMC CC-2705

Catalog #: CC-2705

Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 25 million cells

Product Overview

Mononuclear cell (MNC) rich cells from leukapheresis are depleted of RBCs and platelets. MNC pools contain, on average, 50% T cells, 20% monocytes, 10% B cells, 10% NK cells and may contain up to 5% granulocytes. Cells are cryopreserved in Lonza cryopreservation media. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols.

来自白细胞分离术的富含单核细胞 (MNC) 的细胞耗尽了红细胞和血小板。 MNC 池平均含有 50% 的 T 细胞、20% 的单核细胞、10% 的 B 细胞、10% 的 NK 细胞，并且可能含有高达 5% 的粒细胞。 细胞在 Lonza 冷冻保存培养基中冷冻保存。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis (CoA) is provided for each cell lot purchased

Applications

  • ELISPOT
  • Proliferation assays
  • Immunogenicity assays
  • Cell therapy process development
  • Humanized mice
  • Biomarker discovery

Content & Storage

Content

1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 25 million cells

Instructions, SDS, CoA

lonza,原代细胞和干细胞,D-HBE-COPD 患病人支气管/气管上皮细胞B-ALITM 的 COPD

Catalog #: 00195275S

Product Overview

Chronic bronchitis is marked by long-term cough and excess mucous. Emphysema leads to the destruction of the lungs over time. Most people with COPD have a combination of both conditions. Usually COPD is associated with bronchial epithelium changes.

Cryopreserved diseased human bronchial epithelial cells from donors diagnosed with COPD are screened for air liquid interface differentiation. These cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Our catalog number 00195275S are suitable for air-liquid interface studies and are guaranteed to promote full differentiation of the airway epithelium expressed by the formation of a polarized epithelium with good barrier function (transepithelial resistance), secretory phenotype (mucin secretion) and ciliogenesis. Differentiation usually will occur by day 22 in culture.

Benefits

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

 

Content & Storage

Content

1 x Cryopreserved ampule containing ≥ 500,000 cells

Instructions, SDS, CoA
Related Products
NHBE – Human Bronchial/Tracheal Epithelial Cells for B-ALITM Culture CC-2540S

NHBE – 用于 B-ALITM 培养的人支气管/气管上皮细胞 CC-2540S

D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD for B-ALITM   This product is no longer available for sale.
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540

NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞

D-SAEC-COPD – Diseased Small Airway Epithelial Cells – COPD CC-2934

D-SAEC-COPD – 患病小气道上皮细胞 – COPD CC-2934

D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD for B-ALITM   This product is no longer available for sale.
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma 00194911

D-HBE-As – 患病的人支气管/气管上皮细胞 – 哮喘 00194911

D-HBE-CF – Diseased Human Bronchial/Tracheal Epithelial Cells – Cystic Fibrosis 00196979
D-HBE-CF – 患病的人支气管/气管上皮细胞 – 囊性纤维化 00196979

lonza CC-2580,CC-2901,原代细胞和干细胞,HSMM – 人体骨骼肌成肌细胞

Catalog #: CC-2580

Cryopreserved ampule of Human Skeletal Muscle Myoblasts containing ≥ 500,000 cells

Product Overview

CloneticsTM Human Skeletal Muscle Myoblasts (HSMM) are isolated from normal donors. HSMMs are isolated from the upper arm or leg muscle tissue and are sold in second passage. HSMMs can be differentiated to form myotubes in culture.

CloneticsTM HSMMs are guaranteed through 10 population doublings and to differentiate to form multinucleated myotubes in culture. All cell types test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.

CloneticsTM 人类骨骼肌成肌细胞 (HSMM) 是从正常供体中分离出来的。 HSMM 是从上臂或腿部肌肉组织中分离出来的，并在第二代出售。 HSMM 可以在培养中分化形成肌管。

CloneticsTM HSMM 保证通过 10 次群体倍增并在培养中分化形成多核肌管。 所有细胞类型的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。

Benefits

Applications  

  • Cellular development
  • Differentiation
  • Diabetes
  • Insulin uptake or resistance
  • Tissue repair
  • Drug development
  • Myotube development
  • Co-culture with other cell types

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products

HSMM – Human Skeletal Muscle Myoblasts
SkMC – Human Skeletal Muscle Cells CC-2561
D-HSMM – Diseased Human Skeletal Muscle Myoblasts – Diabetes Type I CC-2900
D-HSMM – Diseased Human Skeletal Muscle Myoblasts – Diabetes Type II CC-2901
HSMM – Human Skeletal Muscle Myoblasts
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5012

P5 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-5032

HSMM – Human Skeletal Muscle Myoblasts
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5024
HSMM – Human Skeletal Muscle Myoblasts
P5 Primary Cell 4D-Nucleofector™ X Kit S V4XP-5032

P5 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-5032

HSMM – Human Skeletal Muscle Myoblasts
P5 Primary Cell 96-well Nucleofector™ Kit V4SP-5096

P5 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-5096

HSMM – Human Skeletal Muscle Myoblasts
P5 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-5960
HSMM – Human Skeletal Muscle Myoblasts
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5002

P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5002

HSMM – Human Skeletal Muscle Myoblasts
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5010
 P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5010

HSMM – Human Skeletal Muscle Myoblasts

Data
HSMM – Human Skeletal Muscle Myoblasts

HSMM – Human Skeletal Muscle Myoblasts

HSMM – Human Skeletal Muscle Myoblasts
HSMM – Human Skeletal Muscle Myoblasts

HSMM – Human Skeletal Muscle Myoblasts

Differentiated skeletal myotubes were stained with Anti-Actin antibody (BioSensis, M-996) used at 1:500 dilution. Secondary antibody VECTDI2488 DyLight 488 (Vector Laboratories). Nuclei were satined with Hoechst stain (blue).

 分化的骨骼肌管用 1:500 稀释度使用的抗肌动蛋白抗体 (BioSensis, M-996) 染色。 二抗 VECTDI2488 DyLight 488（Vector Laboratories）。 细胞核用 Hoechst 染色剂（蓝色）染色。

lonza,CC-2511,原代细胞和干细胞,NHDF-Ad – 人皮肤成纤维细胞

Catalog #: CC-2511

Cryopreserved ampule of Normal Adult Human Dermal Fibroblasts (NHDF-Ad) containing ≥ 500,000 cells

 

Product Overview

Human Dermal Fibroblasts are available from either adult skin (NHDF-Ad) or neonatal foreskin (NHDF-Neo). They are derived from the dermis of normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture. Normal Human Epidermal Keratinocytes (00192627) from matched donors are available for certain lots

Lonza’s human dermal fibroblasts are guaranteed through 15 population doublings when using FGMTM-2 Growth Media. Dermal fibroblasts are characterized by morphological observation throughout serial passage.

All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

人类皮肤成纤维细胞可从成人皮肤 (NHDF-Ad) 或新生儿包皮 (NHDF-Neo) 中获得。 它们来源于正常人类新生儿包皮或成人皮肤的真皮。 它们在原代培养结束时被冷冻保存。 来自匹配供体的正常人表皮角质形成细胞 (00192627) 可用于某些批次

使用 FGMTM-2 生长培养基时，Lonza 的人类真皮成纤维细胞可通过 15 倍的种群倍增得到保证。 真皮成纤维细胞的特征在于贯穿连续传代的形态观察。

所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Applications

  • ECM protein analysis
  • Wound healing
  • Collagen metabolism
  • Cosmetics
  • Skin therapy/models

 

Content & Storage

Content

1 x Cryopreserved ampule of NHDF-Ad containing ≥ 500,000 cells

Related Products

NHDF-Ad - Human Dermal Fibroblasts, Adult
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult CC-2543
NHDF-Ad - Human Dermal Fibroblasts, Adult
NHDF-Neo – Human Dermal Fibroblasts, Neonatal CC-2509
NHDF-Ad - Human Dermal Fibroblasts, Adult
NHEK-Ad – Human Epidermal Keratinocytes, Adult, Single Donor 00192627
NHDF-Ad - Human Dermal Fibroblasts, Adult
NHEM-Ad – Human Epidermal Melanocytes, Adult CC-2586
NHDF-Ad - Human Dermal Fibroblasts, Adult
FGM™-2 Fibroblast Growth Medium-2 BulletKit™ CC-3132
NHDF-Ad - Human Dermal Fibroblasts, Adult
P2 Primary Cell 4D-Nucleofector™ X Kit L V4XP-2012
NHDF-Ad - Human Dermal Fibroblasts, Adult
P2 Primary Cell 4D-Nucleofector™ X Kit L V4XP-2024
NHDF-Ad - Human Dermal Fibroblasts, Adult
P2 Primary Cell 4D-Nucleofector™ X Kit S V4XP-2032
NHDF-Ad - Human Dermal Fibroblasts, Adult
P2 Primary Cell 96-well Nucleofector™ Kit V4SP-2096
NHDF-Ad - Human Dermal Fibroblasts, Adult
P2 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-2960
NHDF-Ad - Human Dermal Fibroblasts, Adult
P2 Primary Cell 384-well Nucleofector™ Kit V5SP-2002
NHDF-Ad - Human Dermal Fibroblasts, Adult
P2 Primary Cell 384-well Nucleofector™ Kit V5SP-2010

NHDF-Ad - Human Dermal Fibroblasts, Adult

NHDF-Ad – Human Dermal Fibroblasts, Adult

Immunohistochemistry staining of normal human adult dermal fibroblasts (CC-2511) epanded into a 24 well plate. Cells were stained with primary antibody Anti-Vimentin AB24525 (AbCam) and secondary antibody Alexa 594, Goat anti Chicken, A11042 Invitrogen was used at 1:200 dilution.

NHDF-Ad - Human Dermal Fibroblasts, Adult

lonza,原代细胞和干细胞,CC-3107,KGM™-2 角质形成细胞生长培养基-2 BulletKit™

Catalog #: CC-3107

Culture system containing KBMTM-2 Basal Medium (CC-3103) and KGMTM-2 SingleQuotsTM Supplements (CC-4152) required for growth of Keratinocytes

Product Overview

KGMTM-2 角质形成细胞生长培养基 BulletkitTM 也作为单独的基础培养基和补充剂出售。

角质形成细胞生长培养基 2 已针对无血清环境中的角质形成细胞增殖进行了优化，通常提供比 KGMTM 培养基更高的生长速率。

CloneticsTM 培养基产品专为支持人和动物原代细胞的生长而设计。 媒体系统已针对各种细胞系统专门开发和优化。

Content & Storage

Content

1 x KBMTM-2 Basal Medium (CC-3103), 500 mL

1 x KGMTM-2 SingleQuotsTM Supplement Pack (CC-4152) containing:

1 x Orange Cap Vial with BPE, 2.00 mL

1 x Green Cap Vial with hEGF, 0.50 mL

1 x Lilac Cap Vial with Insulin, 0.50 mL

1 x Natural Cap Vial with Hydrocortisone, 0.50 mL

1 x Natural Cap Vial with Transferrin, 0.50 mL

1 x Amber Vial with Epinephrine, 0.50 mL

1 x Red Cap Vial with GA-1000, 0.50 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – German
SDS – Italian
SDS – Dutch
SDS – French
SDS – Spanish
SDS – English

lonza CC-4127,原代细胞和干细胞,REGMTM 肾上皮细胞生长培养基 SingleQuotsTM 试剂盒

Catalog #: CC-4127

Supplements and growth factors required for culture of Renal Epithelial Cells (basal media sold separately)

Product Overview

REGMTM SingleQuotsTM 试剂盒是单独出售的组件，不包括基础培养基。 这些是 REGMTM 肾上皮细胞生长培养基 BulletKitTM（目录号 CC-3190）的一部分。 由基础培养基和补充剂组成的完整 REGMTM BulletKitTM 可以使用目录号 CC-3190 一起购买。

REGMTM 肾上皮细胞生长培养基 BulleKitTM 用于维持和扩增大多数肾上皮细胞。 REGMTM 含有 0.5% 的血清。

Content & Storage

Content

1 x REGMTM SingleQuotsTM Supplement Pack (CC-4127) containing:

1 x Green Cap Vial with hEGF, 0.50 mL

1 x Lilac Cap Vial with Insulin, 0.50 mL

1 x Natural Cap Vial with Hydrocortisone, 0.50 mL

1 x Red Cap Vial with GA-1000, 0.50 mL

1 x Bottle with FBS, 2.50 mL

1 x Natural Cap Vial with Transferrin, 0.50 mL

1 x Amber Vial with Triiodothyronine, 0.50 mL

1 x Amber Vial with Epinephrine, 0.50 mL

 

Related Products
HRCE – Human Renal Cortical Epithelial Cells CC-2554

HRCE – 人肾皮质上皮细胞 CC-2554

HRE – Human Renal Epithelial Cells CC-2556

HRE – 人肾上皮细胞 CC-2556

REGMTM Renal Epithelial Cell Growth Medium SingleQuotsTM Kit
RPTEC – Human Renal Proximal Tubule Epithelial Cells CC-2553
D-RPTEC – Diseased Human Renal Proximal Tubule Epithelial Cells – Diabetes Type II CC-2925

D-RPTEC – 患病的人肾近端小管上皮细胞 – II 型糖尿病 CC-2925

REGMTM Renal Epithelial Cell Growth Medium SingleQuotsTM Kit
REGM™ Renal Epithelial Cell Growth Medium BulletKit™ CC-3190
 REGM™ 肾上皮细胞生长培养基 BulletKit™ CC-3190

lonza00193514 ,原代细胞和干细胞,B-ALI™ 支气管气液接口中号 BulletKit™

Catalog #: 00193514

Culture system containing B-ALITM Basal Media (00193516), B-ALITM Differentiation Media (00193517) and B-ALITM SingleQuotsTM Supplements (00193515) required for air-liquid interface differentiation of bronchial epithelial cells.

包含支气管上皮细胞气液界面分化所需的 B-ALITM 基础培养基 (00193516)、B-ALITM 分化培养基 (00193517) 和 B-ALITM SingleQuotsTM 添加剂 (00193515) 的培养系统。

Product Overview

B-ALITM Bronchial Air Liquid Interface Medium BulletKitTM provides scientists with a unique tool to investigate new areas of airway epithelial research in a more physiologically relevant way.

By using an air-liquid interface culture, the next generation tool of in vitro respiratory research, scientists can explore bronchial airway epithelial cell differentiation and apply therapies in novel ways. The B-ALITM BullekitTM is made up of a air liquid interface Growth and Differentiation Medium SingleQuotsTM Kit, Growth Basal Medium and Differentiation Basal Medium.

Our B-ALITM BulletKitTM promotes full differentiation of the airway epithelium expressed by the formation of a polarized epithelium with good barrier function (transepithelial resistance), secretory phenotype (mucin secretion) and ciliogenesis.  When used with our guaranteed bronchial epithelial cells(CC-2540S)

B-ALITM Bronchial Air Liquid Interface Medium BulletKitTM 为科学家提供了一种独特的工具，可以以更生理相关的方式研究气道上皮研究的新领域。

通过使用气-液界面培养（体外呼吸研究的下一代工具），科学家们可以探索支气管气道上皮细胞分化并以新的方式应用治疗。 B-ALITM BullekitTM 由气液界面生长和分化培养基 SingleQuotsTM 套件、生长基础培养基和分化基础培养基组成。

我们的 B-ALITM BulletKitTM 促进气道上皮的完全分化，通过形成具有良好屏障功能（跨上皮抗性）、分泌表型（粘蛋白分泌）和纤毛发生的极化上皮表达。 当与我们保证的支气管上皮细胞（CC-2540S）一起使用时

Content & Storage

Content

1 x B-ALITM Basal Media (00193516), 250 mL

1 x B-ALITM Differentiation Media (00193517), 500 mL

1 x B-ALITM SingleQuotsTM Supplement Pack (00193515) containing:

1 x Natural Cap Vial with Transferrin, 0.75 mL

1 x Amber Vial with B-ALI-Inducer, 1.00 mL

1 x Bottle BPE, 3.00 mL

1 x Amber Vial with Epinephrine, 0.75 mL

1 x Red Cap Vial with GA-1000, 0.75 mL

1 x Green Cap Vial with hEGF, 0.75 mL

1 x Natural Cap Vial with Hydrocortisone, 0.75 mL

1 x Lilac Cap Vial with Insulin, 0.75 mL

1 x Amber Vial with Retinoic Acid, 0.75 mL

1 x Amber Vial with Triiodothyronine, 0.75 mL

 

Related Products
B-ALI™ Bronchial Air-Liquid Interface Medium BulletKit™
BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™ CC-3170
 BEGM™ 支气管上皮细胞生长培养基 BulletKit™ CC-3170

lonza CC-3131,原代细胞和干细胞,FBM™ 成纤维细胞生长基础培养基500 mL

Catalog #: CC-3131

Basal media required for growth of Fibroblasts, 500 mL (supplements and growth factors sold separately).

 

 

Product Overview

FBMTM basal medium is a component sold separately. This is part of the FGMTM -2 and FGMTM -3 Fibroblast Growth Media BulletKitsTM (Catalog Numbers CC-3132 and CC-4526, respectively) which consist of basal medium and supplements packaged together.

FGMTM-2 Fibroblast Growth Medium-2 (CC-3132) was developed to support the growth of most primary human fibroblasts including lung and dermal fibroblasts. FGMTM-2 Medium contains 2% serum.

FGMTM-3 Fibroblast Growth Medium-3 (CC-4526) was developed specifically for cardiac fibroblasts. FGMTM-3 Medium contains contains 10% serum.

Purchase our Lung Fibroblast Cell Culture Kit (catalog no. CC-2512B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of lung fibroblast cells (catalog no. CC-2512) and FGMTM-2 BulletKitTM Growth Media (catalog no. CC-3132)

FBMTM 基础培养基是单独出售的组件。 这是 FGMTM -2 和 FGMTM -3 成纤维细胞生长培养基 BulletKitsTM（目录号分别为 CC-3132 和 CC-4526）的一部分，由包装在一起的基础培养基和补充剂组成。

FGMTM-2 Fibroblast Growth Medium-2 (CC-3132) 旨在支持大多数原代人类成纤维细胞的生长，包括肺和真皮成纤维细胞。 FGMTM-2 培养基含有 2% 血清。

FGMTM-3 Fibroblast Growth Medium-3 (CC-4526) 专为心脏成纤维细胞开发。 FGMTM-3 培养基含有 10% 血清。

购买我们的肺成纤维细胞培养试剂盒（目录号 CC-2512B）并获得细胞和培养基的折扣价。 该试剂盒由冷冻保存的肺成纤维细胞安瓿（货号 CC-2512）和 FGMTM-2 BulletKitTM 生长培养基（货号 CC-3132）组成

Content & Storage

Content

1 x FBMTM Basal Medium, 500 mL

Related Products
FBM™ Fibroblast Growth Basal Medium, 500 mL
NHDF-Neo – Human Dermal Fibroblasts, Neonatal CC-2509

NHDF-Neo – 人类皮肤成纤维细胞，新生儿 CC-2509

FBM™ Fibroblast Growth Basal Medium, 500 mL
NHDF-Ad – Human Dermal Fibroblasts, Adult CC-2511
D-HLF-As – Diseased Human Lung Fibroblast Cells – Asthma 00194912

D-HLF-As – 患病的人肺成纤维细胞 – 哮喘 00194912

D-HLF-COPD – Diseased Human Lung Fibroblast Cell – COPD 00195277

D-HLF-COPD – 患病的人肺成纤维细胞 – COPD 00195277

FBM™ Fibroblast Growth Basal Medium, 500 mL
NHLF – Human Lung Fibroblasts CC-2512

NHLF – 人肺成纤维细胞 CC-2512

NHCF-A – Human Atrial Cardiac Fibroblasts CC-2903

NHCF-A – 人心房心脏成纤维细胞 CC-2903

FBM™ Fibroblast Growth Basal Medium, 500 mL
FGM™-2 Fibroblast Growth Medium-2 BulletKit™ CC-3132

FGM™-2 成纤维细胞生长培养基-2 BulletKit™ CC-3132

FBM™ Fibroblast Growth Basal Medium, 500 mL
NHCF-V – Human Ventricular Cardiac Fibroblasts CC-2904

NHCF-V – 人心室心脏成纤维细胞 CC-2904

FBM™ Fibroblast Growth Basal Medium, 500 mL
FGM™-3 Cardiac Fibroblast Growth Medium-3 BulletKit™ CC-4526
FGM™-3 心脏成纤维细胞生长培养基-3 BulletKit™ CC-4526

lonza,原代细胞和干细胞,NoSpinTM HepaRGTM

Catalog #: NSHPRG

Fully differentiated HepaRGTM cells supplied in a format that does not require centrifugation following thaw. Each ampule contains ≥8.0 million cells.

Product Overview

HepaRGTM Cells are fully-functional, adult-phenotype, human hepatic cells. HepaRGTM Cells exhibit many characteristics similar to primary human hepatocytes and can be used for many of the same applications.  The increased metabolic activity of HepaRG Cells compared to HepG2 enables more predictive value of higher throughput liver toxicity assays conducted in early drug discovery.

The NoSpin HepaRGTM Cells are HepaRG Cells that are provided in a convenient, terminally differentiated, cryopreserved format that does not require the user to centrifuge, resuspend, or count the cells after thawing. This eliminates multiple steps that introduce qualitative and quantitative variability and benefits high-volume labs by reducing the time and effort needed to perform an assay.

Each lot has consistent yield, viability, and functionality across multiple applications.

Using HepaRGTM Cells has never been easier!

Note: The NoSpin HepaRGTM Cells are fully differentiated and non-proliferating.  For the proliferating and non-differentiated version of HepaRGTM Cells, please contact Biopredic International directly.

HepaRGTM 细胞是功能齐全的成人表型人类肝细胞。 HepaRGTM 细胞表现出许多与原代人肝细胞相似的特性，可用于许多相同的应用。与 HepG2 相比，HepaRG 细胞增加的代谢活性使得在早期药物发现中进行的更高通量肝毒性测定具有更高的预测价值。

NoSpin HepaRGTM 细胞是 HepaRG 细胞，以方便的、最终分化的、冷冻保存的形式提供，无需用户在解冻后离心、重悬或计数细胞。这消除了引入定性和定量可变性的多个步骤，并通过减少执行检测所需的时间和精力来使大容量实验室受益。

每个批次在多个应用程序中都具有一致的产量、可行性和功能。

使用 HepaRGTM 细胞从未如此简单！

注意：NoSpin HepaRGTM 细胞是完全分化且不增殖的。对于 HepaRGTM 细胞的增殖和非分化版本，请直接联系 Biopredic International。

Benefits

  • Hepatoctye-like morphology and function
  • Widely accepted as screening tool for liver toxicity
  • No centrifugation step required for higher throughput
  • Greater metabolic activity than HepG2 cells

Applications

  • Drug metabolism
  • infectious disease
  • ADMETox

Content & Storage

Content

1 x Cryopreserved ampule NoSpinTM HepaRGTM containing ≥8 million cells

Data
NoSpinTM HepaRGTM (8.0M cells/vial)

HepaRG CYP Induction chart

Chart displaying cyp induction of HepaRG cells

NoSpinTM HepaRGTM (8.0M cells/vial)

NoSpinTM HepaRGTM (8.0M cells/vial)

The importance of cell-subtype selection in creation of a 3D liver fibrosis model

Data sheet detailing 3D liver fibrosis model in collaboration with Viskol

lonza,lonza 2W-200,原代细胞和干细胞,人外周血 CD4+ T 细胞

Catalog #: 2W-200

Cryopreserved ampule of Human Peripheral Blood CD4+ T Cells containing ≥10 million cells, negatively selected.

Product Overview

CD4+ T cells are also known as helper T cells because they act on other cells of the immune system to promote various aspects of the immune response, including immunoglobulin isotype switching and affinity maturation of the antibody response, macrophage activation and enhanced activity of natural killer cells and cytotoxic T cells.

CD4+ T 细胞也被称为辅助 T 细胞，因为它们作用于免疫系统的其他细胞以促进免疫反应的各个方面，包括免疫球蛋白同种型转换和抗体反应的亲和力成熟、巨噬细胞活化和增强自然杀伤细胞的活性 和细胞毒性 T 细胞。

Cells You Can Count on to Perform

Lonza CD4+T Cells are isolated from peripheral blood of healthy donors using negative immunomagnetic separation.  Cryopreserved CD4+ T cells are guaranteed ≥ 90% pure for CD4+ T cells, as assessed by flow cytometry. They are guaranteed to contain ≥ 10 million viable cells per vial. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Cells are available from multiple donors. Please contact Customer Service for details on available inventory and donor demographic characteristics (age, gender, and ethnicity).

Choices in Cell Type and Tissue Source

Hematopoietic and immune research requires cells from different donors and tissue sources. That’s why Lonza offers many different peripheral blood cell types. If you need different cell types from the same donor, you can either isolate from Lonza cryopreserved PBMCs or contact Customer Service to inquire about cells matched from the same donor. In addition, if you are interested in more naive T cells, unprocessed bone marrow, autologous bone marrow and whole blood from the same donor, bone marrow CD34+ cells, or cord blood CD34+ cells might be good solutions.

Supported by Lonza Media LGM-3TM

Lymphocyte Growth Medium is recommended for optimal performance. Please contact Scientific Support for thawing and culturing guidance. Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

Benefits

– Test negative for mycoplasma, bacteria, yeast, and fungi
– cells from a variety of donors available
– Certificate of Analysis provided for each lot purchased
– HLA  typing available

Content & Storage

Content

1 x Cryopreserved ampule of Human CD4+ T Cells containing ≥10 million cells

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Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 Million
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702
Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 Million
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012

P3 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-3012

Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 Million
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024

P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024

Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 Million
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 Million
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-3096

Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 Million
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 Million
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 Million
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 Million
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza,lonza CC-2701,原代细胞和干细胞,NHDC-人类树突状细胞

Catalog #: CC-2701

Cryopreserved ampule of Normal Human Dendritic Cells (NHDC) containing ≥2.5 million cells

含有≥250 万个细胞的正常人树突状细胞 (NHDC) 冻存安瓿

Product Overview

树突状细胞 (DC) 通过处理来自病原体的抗原并将抗原呈递给 T 细胞以启动免疫反应，从而充当免疫系统的信使细胞。 PoieticsTM 正常人树突状细胞经过一组特定表面标志物的测试，包括 CD11c+、CD86+、CD80+。 HLADR+ 和 CD14- 提供细胞的详细表征。这些细胞可以在含有适当细胞因子（IL-4 和 GM-CSF）的培养物中存活长达 7 天，以将细胞维持为未成熟树突状细胞 (iDC)。通过添加 TNF-α 和 IL-1β，细胞可以进一步分化为成熟的树突状细胞。

应用： 抗原呈递和摄取 初级研究 药理​​学 药物诱导的细胞因子分泌免疫抑制

冷冻保存的 PoieticsTM 正常人树突状细胞 (NHDC) 保证每安瓿含有 ≥ 300 万个活细胞。对 CD80、CD86、HLA-DR、CD11c 和 CD14 的表达水平进行了测试，仅供参考。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。推荐培养基：LGM-3TM 淋巴细胞生长培养基 + 50 ng/ml GM-CSF + 50 ng/ml IL-4

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Benefits

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

 

Content & Storage

Content

1 x Cryopreserved ampule of NHDC containing ≥2.5 million cells

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NHDC - Human Dendritic Cells
LGM-3TM Lymphocyte Growth Medium-3 CC-3211
NHDC - Human Dendritic Cells
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702
NK – Human Natural Killer Cells 2W-502
Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 Million 2W-200
Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 100,000 2M-101
Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000 2M-101B
Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 300,000 2M-101A
Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 1 Million 2M-101C
Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 2 Million 2M-101D

人骨髓 CD34+ 祖细胞，冷冻保存，200 万个 2M-101D

Human Bone Marrow Mononuclear Cells (MNCs) (300 Million Cells) 2M-125E
Human Bone Marrow MNCs, Cryopreserved, 5 Million 2S-101D
NK – Human Natural Killer Cells, Cryopreserved, Negative Selection, 5 Million 2W-501

NK – 人类自然杀伤细胞，冷冻保存，阴性选择，500 万个 2W-501

NHDC - Human Dendritic Cells
Unprocessed Human Bone Marrow, Fresh, 10 mL 1M-105

未加工的人骨髓，新鲜，10 mL 1M-105

NHDC - Human Dendritic Cells
Unprocessed Human Bone Marrow, Fresh, 25 mL 1M-125

未加工的人骨髓，新鲜，25 mL 1M-125

NHDC - Human Dendritic Cells
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza,lonza CC-2550,原代细胞和干细胞,NHAC-kn-人膝关节软骨细胞

Catalog #: CC-2550

Cryopreserved ampule of Normal Human Knee Articular Chondrocytes (NHAC-kn) containing ≥ 750,000 cells

Product Overview

CloneticsTM Normal Human Articular Chondrocytes (NHAC-kn) are isolated from tissue taken from the human knee.

Cryopreserved CloneticsTM Normal Human Articular Chondrocytes are guaranteed through 15 population doublings. After differentiation, they are guaranteed positive for collagen type II and sulfated proteoglycans. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

CloneticsTM 正常人关节软骨细胞 (NHAC-kn) 是从取自人膝关节的组织中分离出来的。

冷冻保存的 CloneticsTM 正常人关节软骨细胞通过 15 次群体倍增得到保证。 分化后，保证它们对 II 型胶原蛋白和硫酸化蛋白多糖呈阳性反应。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Recommended medium:

  • CGMTM Chondrocyte Growth Medium for expansion
  • CDMTM Chondrocyte Differentiation Medium  for differentiation of human chondrocytes

Articular cartilage is characterized by high wear resistance and poor regenerative qualities. It is responsible for much of the compressive resistance and load bearing qualities of the knee joint.

Applications

  • Osteoarthritis
  • Cartilage failure
  • Cellular matrix linkage rupture
  • Chondrocyte protein synthesis inhibition
  • Chondrocyte apoptosis

Content & Storage

Content

1 x Cryopreserved ampule of NHAC-kn containing ≥750,000 cells

Instructions, SDS, CoA
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NHAC-kn – Human Knee Articular Chondrocytes
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
NHAC-kn – Human Knee Articular Chondrocytes
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024

P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024

NHAC-kn – Human Knee Articular Chondrocytes
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

NHAC-kn – Human Knee Articular Chondrocytes
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-3096

NHAC-kn – Human Knee Articular Chondrocytes
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

NHAC-kn – Human Knee Articular Chondrocytes
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

NHAC-kn – Human Knee Articular Chondrocytes
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza,lonza CC-2538,原代细胞和干细胞,NHOst-Human Osteoblasts

Catalog #: CC-2538

Cryopreserved ampule of Normal Human Osteoblasts (NHOst) containing ≥ 500,000 cell

Product Overview

Cryopreserved CloneticsTM Normal Human Osteobalsts (NHOst) are guaranteed through 10 population doublings, stain positive for alkaline phosphatase and mineralization.

All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

冷冻保存的 CloneticsTM 正常人骨剂 (NHOst) 可通过 10 次种群倍增、碱性磷酸酶和矿化染色呈阳性来保证。

所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Benefits

  • Ready-To-Use
  • No tissue dissection required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Content & Storage

Content

1 x Cryopreserved ampule of NHOst containing ≥ 500,000 cells

Instructions, SDS, CoA
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大鼠颅骨成骨细胞 R-OST-583

lonza 00191027,原代细胞和干细胞,HUVEC-XL-人脐静脉内皮细胞

Catalog #: 00191027
HUVEC-XL, Pooled, ≥10 million cells/vial
Overview

Product Overview

Lonza’s Cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed to express CD31/105 and survive in culture through 15* population doublings (*except HUVEC-XLTM guaranteed through 5 PD).

All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

HUVEC-XL size consists of 10 million cells/amp achieved by pooling donors. This option is designed to support studies that require large volume of cells while still offering cost-benefits to researchers.

Our pooled vs. single donor options allow researchers to study or exclude donor variability from their experiments. Depending upon the study, pooled version is typically utilized to reduce donor variability while single donor option allows researchers to assess donor-specific differences.

Not All HUVECs Are Created Equal – Which Type Is Suitable for Your Research?

  • CC-2517 (single donor), CC-2519 (pooled donor) – Both isolated in EGMTM Media, in absence of defined growth factors. Contains BBE. No exogenous VEGF
  • CC-2935 (single donor) – Isolated in EGMTM – Plus Media: Improved version of part number CC-2517. Offers faster proliferation rates than HUVEC isolated in EGMTM Media. No exogenous VEGF
  • C2517A (single donor), C2519A (pooled donor) 00191027 (XL size) – Isolated in EGMTM-2 Media, in presence of growth factors such as VEGF, rhFGF, rhEGF, and others. Does not contain BBE
  • C2517AS (single donor), C2519AS (pooled donor) – Pre-screened HUVECs in EGMTM-2 Media. Screened for angiogenesis markers and test positive for expression of eNOS, Axl, Tie-2 and VEGFr2

 

Benefits

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

 

Content & Storage

Content

1 x Cryopreserved ampule HUVEC containing ≥ 10 million cells/vial

Instructions, SDS, CoA
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HAEC – Human Aortic Endothelial Cells CC-2535
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HCAEC – 人冠状动脉内皮细胞 CC-2585

HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2 C2517A
HUVEC-XL – Human Umbilical Vein Endothelial Cells, Large Volume, Pooled
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2 C2519A

HUVEC – 人脐静脉内皮细胞，混合，在 EGM™-2 C2519A 中

HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™ CC-2517

HUVEC – EGM™ CC-2517 中的人脐静脉内皮细胞，单一供体

HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™ CC-2519
HUVEC – Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus CC-2935
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2, Prescreened for Angiogenesis C2519AS
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2, Prescreened for Angiogenesis C2517AS
HUVEC – 人脐静脉内皮细胞，单一供体，在 EGM™-2 中，预先筛选血管生成 C2517AS

lonza PT-4105,原代细胞和干细胞,MSCGM™ SingleQuots™ 补充剂和生长因子

Catalog #: PT-4105

MSCGMTM SingleQuotsTM  Supplement Kit for proliferation of human bone marrow-derived mesenchymal stem cells.

MSCGMTM SingleQuotsTM 补充试剂盒，用于人骨髓间充质干细胞的增殖。

Product Overview

The MSCGM hMSC SingleQuot Kit is a component of Lonza’s MSCGM BulletKit (catalog no. PT-3001),a serum containing medium designed to proliferate human bone marrow derived mesenchymal cells in an undifferentiated state. The serum used in Lonza’s MSCGMTMhuman Mesenchymal Stem Cell Growth Medium BulletKitTM Medium is specifically screened to ensure that the serum neither promotes spontaneous differentiation of cells nor inhibits cell differentiation to osteogenic, chondrogenic, or adipogenic lineages (when properly stimulated with an appropriate differentiation media).

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support. *List of cells generated from customer data. Lonza only routinely uses this medium with Lonza’s human bone marrow derived mesenchymal stem cells and can therefore only guarantee the successful use of this media with these cells.’

MSCGM hMSC SingleQuot 试剂盒是 Lonza 的 MSCGM BulletKit（目录号 PT-3001）的一个组成部分，该试剂盒是一种含血清的培养基，旨在在未分化状态下增殖人骨髓来源的间充质细胞。 Lonza 的 MSCGMTMhuman 间充质干细胞生长培养基 BulletKitTM 培养基中使用的血清经过专门筛选，以确保血清既不促进细胞的自发分化，也不抑制细胞向成骨、软骨或脂肪谱系的分化（当用适当的分化培养基适当刺激时）。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。 *从客户数据生成的单元格列表。 Lonza 通常只将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用，因此只能保证这种培养基与这些细胞一起成功使用。

lonza CC-2551,原代细胞和干细胞,HMEC-人乳腺上皮细胞

Catalog #: CC-2551

Cryopreserved ampule of Human Mammary Epithelial Cells (HMEC) containing ≥500,000 cells

 

Product Overview

Lonza’s Human Mammary Epithelial Cells (HMEC) are isolated from adult female breast tissue.  The cells have been primarily utilized to understand the formation of breast cancer. Cryopreserved Human Mammary Epithelial Cells are guaranteed through 15 population doublings, express cytokeratins 14 and 18 and are negative for cytokeratin 19.

Lonza 的人乳腺上皮细胞 (HMEC) 是从成年女性乳腺组织中分离出来的。 这些细胞主要用于了解乳腺癌的形成。 冷冻保存的人乳腺上皮细胞通过 15 次群体倍增，表达细胞角蛋白 14 和 18，对细胞角蛋白 19 呈阴性。

Select publications highlighting use of HMECs:  

  • In an article, Xinyi Guo et al 2014 New J. Phys. 16 105002, the research team utilized Lonza’s HMEC side-by-side with immortalized HMEC and well-established breast cancer cell lines MCF7 and MDA-MB-231 to understand the effect of neighboring cells on the stiffness of cancerous and non-cancerous human mammary epithelial cells.
  • In the article, Elenbaas et al. Genes & Development. 2001, HMECs were propagated in MEGMTM BulletKitTM. HMECs expressing LT and hTERT were infected with a retrovirus encoding H-rasV12 and were introduced in mice. The resulting data showed that HMECs have successfully been transformed to tumorigenicity through the introduction of a limited set of oncogenes.

Purchase our Mammary Epithelial Cell Culture Kit (catalog no. CC-2551B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of mammary epithelial cells (catalog no. CC-2551) and MEGMTM BulletKitTM Growth Media (catalog no. CC-3150)

Benefits

  • Guaranteed through 15 population doublings
  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of HMEC containing ≥500,000 cells

Related Products

HMEC – Human Mammary Epithelial Cells
PrEC – Human Prostate Epithelial Cells CC-2555
HMEC – Human Mammary Epithelial Cells
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
HMEC – Human Mammary Epithelial Cells
MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™ CC-3150
HMEC – Human Mammary Epithelial Cells
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
HMEC – Human Mammary Epithelial Cells
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024
HMEC – Human Mammary Epithelial Cells
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

HMEC – Human Mammary Epithelial Cells
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096
HMEC – Human Mammary Epithelial Cells
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

HMEC – Human Mammary Epithelial Cells
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

HMEC – Human Mammary Epithelial Cells
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

HMEC – Human Mammary Epithelial Cells

Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems

Graph showing the propagation potential of Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems

HMEC – Human Mammary Epithelial Cells

lonza2T-110,原代细胞和干细胞,人破骨细胞前体细胞

Catalog #: 2T-110

Osteoclast Precusors, 1 million cells

Product Overview

骨组织的维持和修复主要归因于两种细胞类型：形成新骨组织的成骨细胞和重新吸收骨组织的破骨细胞。骨矿化/骨降解循环中的缺陷会导致许多与骨相关的疾病，例如骨质疏松症。破骨细胞是大的多核细胞，在骨吸收中发挥积极作用。 PoieticsTM 破骨细胞前体细胞系统设计用于高通量应用，以进行骨质疏松症、骨吸收和其他骨相关疾病的研究。来自骨髓或血液的单核细胞充当破骨细胞的前体。传统上，研究人员驱动纯化的单核细胞群成为破骨细胞，然而，在某些情况下，这可能导致低至 10% 的分化效率。相比之下，Lonza 的破骨细胞前体细胞系统在分化时产生高达 50% 的前体向破骨细胞的转化。冷冻保存的 PoieticsTM 人破骨细胞前体细胞 (OCP) 保证每安瓿含有 ≥ 100 万个活细胞，并且在分化后对破骨细胞活性和 TRAP 染色检测呈阳性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。推荐培养基：OCPTMOcteoclast Precursor BulletKitTM 培养基（提供阴性对照/实验培养基，可用作阴性对照[不添加额外的补充剂]或实验培养基[添加研究人员提供的分化补充剂]和阳性对照培养基保证诱导分化为成熟的、功能活跃的破骨细胞；每瓶 OCPTMBulletKitTM 培养基含有足够的分化培养基，可将大约 150 万个破骨细胞前体细胞 [大约 1.5 个完整的 96 孔板] 完全分化成成熟的、功能活跃的破骨细胞）。 OCPTMMedium 以 BulletKitTM 培养基（目录号 PT-8001）的形式提供，其中包括基础培养基和破骨细胞前体的铺板和分化所需的补充剂。基础培养基（OCPTMOsteoclast Precur.Basal Medium；目录号 PT-8201）和必要的补充剂（OCPTMOsteoclast Precur. SingleQuot Kit；目录号 PT-9501）也可以单独购买。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

lonza CC-2561,CC-2580,原代细胞和干细胞,SkMC-人体骨骼肌细胞

Catalog #: CC-2561

Cryopreserved ampule of Human Skeletal Muscle Cells (SkMC) containing ≥ 500,000 cells

 

Product Overview

CloneticsTM 人类骨骼肌细胞 (SkMC) 是从正常供体中分离出来的。 SkMC 是从妊娠组织中分离出来的，通常是从股四头肌或腰大肌组织中分离出来的。

CloneticsTM SkMC 保证通过 15 次群体倍增并且对结蛋白染色呈阳性。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。

Applications

  • Cellular development
  • Differentiation
  • Diabetes
  • Insulin uptake or resistance
  • Tissue repair
  • Drug development
  • Myotube development
  • Co-culture with other cell types

 

Content & Storage

Content

1 x Cryopreserved ampule of SkMC containing ≥ 500,000 cells

Related Products
SkMC – Human Skeletal Muscle Cells
HSMM – Human Skeletal Muscle Myoblasts CC-2580

HSMM – 人体骨骼肌成肌细胞 CC-2580

SkMC – Human Skeletal Muscle Cells
P4 Primary Cell 4D-Nucleofector™ X Kit L V4XP-4012
SkMC – Human Skeletal Muscle Cells
P4 Primary Cell 4D-Nucleofector™ X Kit L V4XP-4024

P4 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-4024

SkMC – Human Skeletal Muscle Cells
P4 Primary Cell 4D-Nucleofector™ X Kit S V4XP-4032

P4 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-4032

SkMC – Human Skeletal Muscle Cells
P4 Primary Cell 96-well Nucleofector™ Kit V4SP-4096
SkMC – Human Skeletal Muscle Cells
P4 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-4960
SkMC – Human Skeletal Muscle Cells
P4 Primary Cell 384-well Nucleofector™ Kit V5SP-4002

P4 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-4002

SkMC – Human Skeletal Muscle Cells
P4 Primary Cell 384-well Nucleofector™ Kit V5SP-4010
P4 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-4010

lonza,HUCLS,原代细胞和干细胞,星状细胞人类冷冻保存第 1 代

Catalog #: HUCLS
Human Cryopreserved Stellate cells derived from liver

Product Overview

Stellate cells are a resident cell type of the liver primarily functioning to store retinoids. In response to liver damage, stellate cells rapidly lose the stored retinoids and differentiate into a proliferating fibroblast-like cell that begins depositing collagen matrix. This activity causes buildup of collagen in the liver eventually leading to cirrhosis.

Stellate cells can be isolated from liver tissue, enriched, and placed into cell culture. When placed into culture, stellate cells begin to immediately differentiate into the activated state.

Human Cryopreserved Stellate Cells, 100K cells/vial,  are stellate cells enriched from human liver isolations and grown to >80% confluency and then cryopreserved.

Human Stellate Growth Medium (Catalog MCST250) is available to support optimal culture health and growth characteristics of stellate cells.

星状细胞是肝脏的一种常驻细胞类型，主要功能是储存类维生素A。 作为对肝损伤的反应，星状细胞迅速失去储存的类维生素A并分化成增殖的成纤维细胞样细胞，开始沉积胶原蛋白基质。 这种活动会导致肝脏中胶原蛋白的堆积，最终导致肝硬化。

星状细胞可以从肝组织中分离、富集并放入细胞培养物中。 当放入培养物中时，星状细胞开始立即分化成活化状态。

人冷冻保存的星状细胞，100K 细胞/瓶，是从人肝分离物中富集的星状细胞，并生长到 >80% 的汇合度，然后冷冻保存。

人星状生长培养基（目录 MCST250）可用于支持星状细胞的最佳培养健康和生长特性。

Characterization performed:

  • Post-thaw plating to observe characteristic stellate morphology.
  • Flow cytometry using antibodies for Vinculin, Vimentin, and α-SMA

Benefits

  • Low Passage
  • High activation potential

Applications

  • ADMETox
  • Liver Disease
  • NASH

Content & Storage

Content

Cryo Vial

100,000 cells/ vial

Stellate cells, human cryopreserved, passage 1

lonza CC-5024,CC-5034,原代细胞和干细胞,HEPES 缓冲盐溶液500 mL

Catalog #: CC-5024
HEPES Buffered Saline Solution for use in cell culture, 500 mL

Product Overview

HEPES 缓冲盐水溶液 (BSS) 用于中和生长培养基中的复杂蛋白质，这些蛋白质可能在胰蛋白酶消化过程中使胰蛋白酶失活。

填充体积为 100 mL 的 HEPES BSS 是 ReagentPackTM Subculture 试剂盒（目录号 CC-5024）的一个组件。 100 mL 容量也单独出售（目录号 CC-5022）。

Content & Storage

Content

1 x Bottle HEPES Buffered Solution, 500 mL

Related Products
HEPES Buffered Saline Solution, 500 mL
ReagentPack™ Subculture Reagents, 100 mL CC-5034

ReagentPack™ 继代培养试剂，100 mL CC-5034

HEPES Buffered Saline Solution, 500 mL
HEPES Buffered Saline Solution – 100 ml CC-5022
 HEPES 缓冲盐溶液 – 100 ml CC-5022

lonza,CC-2904,原代细胞和干细胞,NHCF-V-人心室心脏成纤维细胞

Catalog #: CC-2904

Cryopreserved ampule of Normal Human Ventricular Cardiac Fibroblasts (NHCF-V) containing ≥ 500,000 cells

Product Overview

Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or ventricle. Cells are isolated from normal, adult heart tissue. Cryopreserved cells are shipped at passage 2.

CloneticsTM NHCF-V & -A are guaranteed through 5 population doublings and express ≥90% collagen I and ≤10% Von Willibrand factor VIII after thaw. CloneticsTM AoAF are guaranteed through 10 population doublings and are negative for alpha smooth muscle actin. All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.

人类心脏相关成纤维细胞包括从升主动脉或降主动脉外部的被膜分离的主动脉外膜成纤维细胞 (AoAF) 和从心房或心室分离的心脏成纤维细胞 (NHCF-A 和 -V)。 细胞是从正常的成人心脏组织中分离出来的。 冷冻保存的细胞在第 2 代运送。

CloneticsTM NHCF-V 和 -A 通过 5 次群体倍增得到保证，解冻后表达 ≥90% 的胶原蛋白 I 和 ≤10% 的 Von Willibrand 因子 VIII。 CloneticsTM AoAF 通过 10 次群体倍增得到保证，并且对 α 平滑肌肌动蛋白呈阴性。 所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。

Benefits

  • Assured for experimental use up to 15 population doublings
  • Express ≥90% collagen I and ≤10% Von Willibrand factor VIII
  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

Applications

  • Vascular diseases
  • Neointimal formation
  • Vascular remodeling
  • Atherosclerosis
  • Hypertension
  • Myocardial repair
  • Extracellular maintenance during cardiac cell / tissue growth
  • Cardiac Fibrosis Paracrine signalling
  • Toxicology
  • Gene expression

Content & Storage

Content

1 x Cryopreserved ampule NHCF-V containing ≥ 500,000 cells/vial

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NHCF-V – Human Ventricular Cardiac Fibroblasts
AoAF – Human Aortic Adventitial Fibroblasts CC-7014
AoAF – 人主动脉外膜成纤维细胞 CC-7014
NHCF-V – Human Ventricular Cardiac Fibroblasts
NHCF-A – Human Atrial Cardiac Fibroblasts CC-2903
NHCF-V – Human Ventricular Cardiac Fibroblasts
HAEC – Human Aortic Endothelial Cells CC-2535

HAEC – 人主动脉内皮细胞 CC-2535

NHCF-V – Human Ventricular Cardiac Fibroblasts
AoSMC – Human Aortic Smooth Muscle Cells CC-2571

AoSMC – 人主动脉平滑肌细胞 CC-2571

NHCF-V – Human Ventricular Cardiac Fibroblasts
FGM™-3 Cardiac Fibroblast Growth Medium-3 BulletKit™ CC-4526
FGM™-3 心脏成纤维细胞生长培养基-3 BulletKit™ CC-4526

lonza,lonza CC-5022,原代细胞和干细胞,HEPES 缓冲盐溶液

Catalog #: CC-5022
HEPES Buffered Saline Solution, 100 mL
Overview

Product Overview

HEPES-Buffered Saline Solution (BSS), is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization.

HEPES-BSS is a component of the ReagentPackTM Subculture kit (catalog number CC-5034). It is also available in a 500 mL size (catalog number CC-5024)

HEPES 缓冲盐溶液 (BSS) 用于中和生长培养基中的复杂蛋白质，这些蛋白质可能会在胰蛋白酶消化过程中使胰蛋白酶失活。

HEPES-BSS 是 ReagentPackTM Subculture 试剂盒（目录号 CC-5034）的一个组件。 它也有 500 mL 规格（目录号 CC-5024）

Content & Storage

Content

1 x Bottle HEPES Buffered Solution 100 mL

lonza,CC-3111,原代细胞和干细胞,KGM™ 角质形成细胞生长培养基 BulletKit™

Catalog #: CC-3111

KGMTM Keratinocyte Growth Medium BulletKitTM culture system contains KBMTM Basal Medium (CC-3101) and KGMTM SingleQuot supplements (CC-4131) required for growth of human keratinocytes.

KGMTM 角质形成细胞生长培养基 BulletKitTM 培养系统包含人类角质形成细胞生长所需的 KBMTM 基础培养基 (CC-3101) 和 KGMTM SingleQuot 补充剂 (CC-4131)。

Product Overview

KGMTM Keratinocyte Growth Medium has been optimized for the proliferation of keratinocytes in a serum-free environment.

Did you know our customers have used KGMTM Media also to culture immortalized corneal epithelial cells? Check out Jolly, et. al. publication that provides some guidelines to do this.

KGMTM 角质形成细胞生长培养基已针对角质形成细胞在无血清环境中的增殖进行了优化。

您知道我们的客户也使用 KGMTM 培养基培养永生化角膜上皮细胞吗？ 看看 Jolly 等。 人。 提供了一些指导方针的出版物。

Content & Storage

Content

1 x KBMTM (CC-3101) Basal Medium, 500 mL

1 x KGMTM (CC-4131) Singlequot Supplement Pack containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL

lonza,lonza PT-5025,原代细胞和干细胞,DPSC-人类牙髓干细胞

Catalog #: PT-5025

Cryopreserved ampule of Human Dental Pulp Stem Cells containing ≥1 million cells

Product Overview

PoieticsTM 人类牙髓干细胞 (DPSC) 是从捐赠者的“智齿”拔牙过程中收集的成年第三磨牙中分离出来的。这些细胞表现出与骨髓来源的间充质干细胞非常相似的表型和功能特征。据报道，人类牙髓干细胞 (DPSC) 可分化出许多不同的谱系，包括软骨形成、成骨、脂肪形成和神经细胞。

人牙髓干细胞 (DPSC) 已在初级传代时冷冻保存，并且 DPSC 牙髓干细胞 BulletKitTM 培养基已针对细胞维持和扩增进行了优化。

冷冻保存的 PoieticsTM 牙髓干细胞 (DPSC) 保证通过 10 次群体倍增，表达 CD105、CD166、CD29、CD90 和 CD73，并且不表达 CD34、CD45 和 CD133。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Recommended Growth Media and Reagents

  • DPSC Dental Pulp Stem Cell BulletKitTM Medium, PT-3005

The DPSC Dental Pulp Stem Cell BulletKitTM Medium is offered as a BulletKitTM Medium (catalog no. PT-3005) which includes both the basal media and the necessary supplements for proliferation of human dental pulp derived mesenchymal stem cells. The basal medium (DPSC Basal Medium; catalog no. PT-3927) and the necessary supplements (DPSC SingleQuot Kit; catalog no. PT-4516) can also be purchased individually.

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.

DPSC 牙髓干细胞 BulletKitTM 培养基以 BulletKitTM 培养基（目录号 PT-3005）的形式提供，其中包括基础培养基和人牙髓衍生间充质干细胞增殖所需的补充剂。 基础培养基（DPSC 基础培养基；目录号 PT-3927）和必要的补充剂（DPSC SingleQuot 试剂盒；目录号 PT-4516）也可以单独购买。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Applications

  • Toxicology/drug screening
  • Regenerative medicine/dentistry/cell therapy
  • Muscular Dystrophy
  • Corneal replacement therapy
  • Cardiovascular disease
  • Comparative stem cell research

Content & Storage

Content

1 x Cryopreserved ampule of Human Dental Pulp Stem Cells containing ≥1 million cells

Related Products
DPSC - Human Dental Pulp Stem Cells
Human Dental Pulp Stem Cell Basal Medium PT-3927
DPSC - Human Dental Pulp Stem Cells
Human Dental Pulp Stem Cell SingleQuots™ Supplements and Growth Factors PT-4516

人牙髓干细胞 SingleQuots™ 补充剂和生长因子 PT-4516

DPSC - Human Dental Pulp Stem Cells
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218

MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

DPSC - Human Dental Pulp Stem Cells
hMSC – Human Mesenchymal Stem Cells PT-2501

hMSC – 人间充质干细胞 PT-2501

DPSC - Human Dental Pulp Stem Cells
MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ PT-3001

MSCGM™ 间充质干细胞生长培养基 BulletKit™ PT-3001

DPSC - Human Dental Pulp Stem Cells
Human Mesenchymal Stem Cell (hMSC) Osteogenic Differentiation Medium BulletKitTM PT-3002

人间充质干细胞 (hMSC) 成骨分化培养基 BulletKitTM PT-3002

DPSC - Human Dental Pulp Stem Cells
Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation Medium BulletKitTM PT-3003

人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM PT-3003

DPSC - Human Dental Pulp Stem Cells
Human Mesenchymal Stem Cell (hMSC) Adipogenic Differentiation Medium BulletKitTM PT-3004

人间充质干细胞 (hMSC) 脂肪分化培养基 BulletKitTM PT-3004

DPSC - Human Dental Pulp Stem Cells
TGF Beta 3 for hMSC Chondrogenic Differentiation Kit PT-4124

用于 hMSC 软骨分化试剂盒 PT-4124 的 TGF Beta 3

DPSC - Human Dental Pulp Stem Cells
hADSC Human Adipose-Derived Stem Cells PT-5006
hADSC 人体脂肪干细胞 PT-5006

lonza 00192906,CC-2504,NHEK-Neo-人表皮角质形成细胞，新生儿，混合

Catalog #: 00192906

Cryopreserved Human Neonatal Epidermal Keratinocytes, Pooled, ≥ 500,000 cells

Product Overview

Cryopreserved Normal Human Epidermal Keratinocytes from pooled donros are guaranteed through 18 population doublings for adult and neonatal cells.

All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

References with Lonza’s Keratinocytes:

  • Protective barrier: Keratinocytes were stimulated with histamine or histamine receptor ligands to show the formation of a defective skin barrier. Gschwandtner M, Mildner M,  Mlitz V,  Gruber F,  Eckhart L, Werfel T, Gutzmer R, Elias PM and Tschachler E.  Histamine suppresses epidermal keratinocyte differentiation and impairs skin barrier function in a human skin model. European Journal of Allergy and Clinical immunology (2013); 68(1): 37–47. 
  • Skin model: Neonatal keratinocytes were cultured in a microfluidic system to precisely examine the morphology, behavior and viability of individual cells. Adrian T. O’Neill, Nancy A. Monteiro-Riviere, and Glenn M. Walker. Characterization of microfluidic human epidermal keratinocyte culture. Cytotechnology (2008); 56(3): 197–207. 
  • Cancer: Keratinocytes were used in this study to demonstrate that dysregulated activation of type I interferon innate immunity is implicated in the molecular processes triggered by anti-EGFR drugs and leading to persistent skin inflammation. Carrie HS, Hong Y, Yunbo L, Jennifer SM, Sridevi B, Gaylene S, Judith AC and Thomas RS.  EGF receptor signaling blocks aryl hydrocarbon receptor-mediated transcription and cell differentiation in human epidermal keratinocytes. Proceedings of National Academy of Sciences (2009); 106(11): 4266-71.
  • Human Papillomaviruses (HPV) mediated skin cancer response: An infection of the skin with human papillomaviruses (HPV) may result in benign tumors with limited growth, which tend to regress spontaneously and causes non-melanoma skin cancer development in epidermodysplasia verruciformis (EV) and immunosuppressed patients.  The present study utilized pooled keratinocytes cultured in KGMTM-Gold Medium from several healthy donors. The data showed that beta PV may increase the number of stem cell-like cells present during early carcinogenesis and thus enable the persistence and accumulation of DNA damage necessary to generate malignant stem cells. Martin H, Adrian B, Cinzia B, Marisa G, John D, Alan S, Herbert P, Ian M and Baki A. Expression of betapapillomavirus oncogenes increases the number of keratinocytes with stem cell-like properties. Journal of Virology (2013); 87(22): 12158-12165. 

Content & Storage

Content

1 x Cryopreserved ampule of Human Neonatal Epidermal Keratinocytes containing ≥ 500,000 cells

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NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled
NHEM-Neo – Human Epidermal Melanocytes, Neonatal CC-2504

NHEM-Neo – 人表皮黑色素细胞，新生儿 CC-2504

NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024

P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024

NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-3096

NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010

NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled

Data
NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled

Growth Curve of NHEK Culture

The growth curve of the Neonatal Human Epidermal Keratinocyte culture displayed in the image shows the expected doubling time of 1 population doubling per 24 hours in the log phase of within the first 2 days of culture, but significantly slows down after 2.5 days and comes finally to a growth stop.

NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled
图中显示的新生儿人表皮角质形成细胞培养物的生长曲线显示，在培养的前 2 天的对数阶段，每 24 小时 1 个种群的预期倍增时间，但在 2.5 天后显着减慢，最终达到 增长停止。

lonza PT-4505,原代细胞和干细胞,ADSC 生长培养基 BulletKit™

Catalog #: PT-4505

含有脂肪干细胞生长所需的 ADSC 基础培养基 (PT-3273) 和 ADSC-GM SinglequotsTM Supplement Kit (PT-4503) 的培养系统

Product Overview

干细胞具有作为未分化细胞类型增殖的独特能力，然后在适当刺激时分化成多个细胞谱系。 Lonza 的 ADSC 人脂肪来源的干细胞生长 BulletKitTM 培养基是一种含有血清的培养基，旨在增殖处于未分化状态的人脂肪来源的间充质干细胞（正常和患病）。

Lonza 的 ADSC 人脂肪衍生干细胞生长 BulletKitTM 培养基中使用的血清经过专门筛选，以确保血清既不促进细胞自发分化，也不抑制细胞分化为成骨、软骨或脂肪谱系（当用适当的分化培养基适当刺激时） .

ADSC 人脂肪源性干细胞生长 BulletKitTM 培养基以 BulletKitTM 培养基（目录号 PT-4505）的形式提供，其中包括基础培养基和人脂肪源性间充质干细胞增殖所需的补充剂。基础培养基（ADSC 基础培养基；目录号 PT-3273）和必要的补充剂（ADSC-GM SQ 试剂盒；目录号 PT-4503）也可以单独购买。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Content & Storage

Content

  •  1 x ADSC Basal Medium (catalog no. PT-3273)
  •  1 x ADSC SingleQuotsTM Growth Supplement Kit (catalog no. PT-4503)
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ADSC Growth Medium BulletKit™
hADSC Human Adipose-Derived Stem Cells PT-5006
ADSC Growth Medium BulletKit™
ADSC – Apidose-Derived Stem Cells Growth Medium SingleQuots™ Supplements and Growth Factors PT-4503
ADSC Growth Medium BulletKit™
ADSC Basal Medium PT-3273
ADSC Growth Medium BulletKit™
Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5007
ADSC Growth Medium BulletKit™
Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5008
ADSC Growth Medium BulletKit™
hMSC – Human Mesenchymal Stem Cells PT-2501
ADSC Growth Medium BulletKit™
MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ PT-3001
ADSC Growth Medium BulletKit™
Human Mesenchymal Stem Cell (hMSC) Osteogenic Differentiation Medium BulletKitTM PT-3002
ADSC Growth Medium BulletKit™
Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation Medium BulletKitTM PT-3003
ADSC Growth Medium BulletKit™
Human Mesenchymal Stem Cell (hMSC) Adipogenic Differentiation Medium BulletKitTM PT-3004
ADSC Growth Medium BulletKit™
PGM-2TM Preadipocyte Growth Medium-2 BulletKitTM PT-8002

PGM-2TM 前脂肪细胞生长培养基-2 BulletKitTM PT-8002

ADSC Growth Medium BulletKit™
TGF Beta 3 for hMSC Chondrogenic Differentiation Kit PT-4124

用于 hMSC 软骨分化试剂盒 PT-4124 的 TGF Beta 3

ADSC Growth Medium BulletKit™
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza CC-2586,CC-2511,原代细胞和干细胞,NHEM-Ad-成人表皮黑色素细胞

Catalog #: CC-2586
NHEM-Ad, Epidermal Melanocytes, Adult
Overview

Product Overview

成人和新生儿人类表皮黑色素细胞 (NHEM) 是位于所有哺乳动物皮肤表皮层的色素生成细胞。黑色素细胞的功能是产生黑色素，黑色素是赋予皮肤颜色的色素。黑色素保护皮肤免受紫外线伤害。皮肤由2个主要层组成，表皮和真皮。表皮由多个角质形成细胞层组成。黑素细胞位于表皮的基底层。星状或树突状黑素细胞形态允许黑素细胞细胞体驻留在表皮的基底层，而树突与表皮上层的角质形成细胞结合。这种关联允许黑色素细胞将黑色素从细胞体通过树突转运到角质形成细胞中，从而导致皮肤着色。

黑素细胞研究有两个主要领域——化妆品和医学。化妆品研究中心专注于改变皮肤颜色的能力。医学研究通常集中在黑色素瘤以及各种皮肤病或相关疾病，如白化病和白癜风、激素治疗、甲状腺疾病、牛皮癣、烧伤和疤痕。

Our Cryopreserved Normal Human Epidermal Melanocytes are guaranteed through 8 population doublings, express Mel-5 and L-Dopa conversion to melanin.  All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Note: Melanocytes are extremely sensitive to over trypsinization. Use Lonza’s Trypsin/EDTA Solution (CC-5012) and dilute 1:1 with Versene®(17-711E) prior to use.

 

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

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NHEM-Ad – Human Epidermal Melanocytes, Adult
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NHEM-Ad – Human Epidermal Melanocytes, Adult
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult CC-2543

HMVEC-dAd – 人真皮微血管内皮细胞 – 成人 CC-2543

NHEM-Ad – Human Epidermal Melanocytes, Adult
NHEK-Ad – Human Epidermal Keratinocytes, Adult, Single Donor 00192627
NHEK-Ad – 人表皮角质形成细胞，成人，单一供体 00192627

lonza CC-2556 HRE-人肾上皮细胞,REGM™ 肾上皮细胞生长培养基 BulletKit™ CC-3190

Catalog #: CC-2556
HRE, Renal Epithelial Cells
Overview

Product Overview

Lonza 的人肾细胞包括从对每种细胞类型特异的各种正常人肾组织中分离出来的细胞。人肾近端小管细胞从近端小管中分离出来，是药物转运蛋白研究的理想选择。人肾上皮细胞 (HRE) 由来自皮质和肾小球区域的细胞组成。人肾皮质细胞 (HRCE) 来自近端和远端小管。

选择大量在没有庆大霉素-两性霉素的情况下分离的肾近端小管上皮细胞是可用的。这些批次在强力霉素中分离。请联系您当地的客户代表购买这些批次。

肾上皮细胞表现出极化形态，这对其功能至关重要。它们重新吸收肾小球滤液中几乎所有的葡萄糖和氨基酸，同时允许其他没有营养价值的物质从尿液中排出。渗透调节和排泄等生理过程可以在体外用 HRE 进行研究。人肾上皮细胞也能够内化细菌，并且位于各种先天性、代谢和炎症性疾病的损伤部位附近。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

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H-RPE – 人视网膜色素上皮细胞 00194987

HRE – Human Renal Epithelial Cells
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540

NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞

HRE – Human Renal Epithelial Cells
REGM™ Renal Epithelial Cell Growth Medium BulletKit™ CC-3190
REGM™ 肾上皮细胞生长培养基 BulletKit™ CC-3190

lonza CC-4526,原代细胞和干细胞,FGM™-3 心脏成纤维细胞生长培养基-3 BulletKit™

Catalog #: CC-4526

含有成纤维细胞生长所需的 FBMTM 基础培养基 (CC-3131) 和 FGMTM-3 SingleQuot 补充剂 (CC-4525) 的培养系统

Product Overview

FGMTM-3 Fibroblast Growth Medium-3 was developed specifically for culturing primary cardiac fibroblasts. The kit contains basal medium and supplements packaged together for convenience to culture fibroblasts. FGMTM-3 Medium contains contains 10% serum.

FGMTM-3 Fibroblast Growth Medium-3 专为培养原代心脏成纤维细胞而开发。 该试剂盒包含包装在一起的基础培养基和补充剂，以方便培养成纤维细胞。 FGMTM-3 培养基含有 10% 血清。

Content & Storage

Content

1 x FBMTM Basal Medium (CC-3131), 500 mL

1 x FGMTM-3 SingleQuotsTM Supplement Pack (CC-4525) containing:

1 x Lilac Cap Vial with Insulin, 0.50 mL

1 x Gray Cap Vial with hFGF-B, 0.50 mL

1 x Red Cap Vial with GA-1000, 0.50 mL

1 x Bottle FBS, 50.00 mL

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FGM™-3 Cardiac Fibroblast Growth Medium-3 BulletKit™
NHCF-A – Human Atrial Cardiac Fibroblasts CC-2903

NHCF-A – 人心房心脏成纤维细胞 CC-2903

FGM™-3 Cardiac Fibroblast Growth Medium-3 BulletKit™
FBM™ Fibroblast Growth Basal Medium, 500 mL CC-3131

FBM™ 成纤维细胞生长基础培养基，500 mL CC-3131

FGM™-3 Cardiac Fibroblast Growth Medium-3 BulletKit™
NHCF-V – Human Ventricular Cardiac Fibroblasts CC-2904

NHCF-V – 人心室心脏成纤维细胞 CC-2904

FGM™-3 Cardiac Fibroblast Growth Medium-3 BulletKit™
FGM™3 Cardiac Fibroblast Growth Medium-3 SingleQuots™ Supplements and Growth Factors CC-4525
FGM™3 心脏成纤维细胞生长培养基-3 SingleQuots™ 补充剂和生长因子 CC-4525

lonza 1M-105,原代细胞和干细胞,未加工的人骨髓10 mL

Catalog #: 1M-105
Fresh Unprocessed Human Bone Marrow, 10 mL
Overview

Product Overview

We Find the Donor. You Find the Cure.

在 Lonza，我们致力于处理物流，因此您可以专注于寻找治疗方法。 为研究界提供新鲜的未加工骨髓，同时维护我们捐赠者的福祉，这是龙沙批准的骨髓捐赠者计划的最前沿。 20 多年来，我们一直在提供您需要的新鲜未加工骨髓，因此您可以专注于寻找治愈方法背后的重要工作。

More Cells

所有用于研究的、未经处理的新鲜骨髓样本每毫升含有超过 1500 万个有核活细胞，为您提供更多细胞，物有所值。 可以以 10 或 25 mL 的数量订购每个供体总共 100 mL。 每个供体最多可订购 100 mL。 请咨询大量新鲜未加工骨髓的定价。

Fresh Delivery

未经处理的骨髓样本永远不会被稀释，并在环境温度下运输，以便第二天交付，因此您的样本新鲜且有活力。 未处理的骨髓订单在周一至周四发货，通常提前 1-2 周安排，具体取决于供体和数量要求。 可提供国际运输，交货时间各不相同。

Relevant Results

样本表示对于确保您为目标人群收集相关结果非常重要。

Lonza 骨髓捐献者计划自 1994 年开始运作。20 多年来，它一直维持着庞大而多样的捐献者库，以实现相关研究成果。 此外，我们还提供来自同一供体的全血和骨髓。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis (CoA) is provided for each cell lot purchased

Content & Storage

Content

1 x Unprocessed Human Bone Marrow, 10 mL

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Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702

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Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 100,000 2M-101

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Human Bone Marrow Stromal Cells, 5 Million 2M-302

人骨髓基质细胞，500 万个 2M-302

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Human Cord Blood CD34+ Progenitor Cells, Cryopreserved, 500,000 cells 2C-101A

人脐带血 CD34+ 祖细胞，冷冻保存，500,000 个细胞 2C-101A

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hMSC – Human Mesenchymal Stem Cells PT-2501
hMSC – 人间充质干细胞 PT-2501

lonza CC-5036,CC-5034,原代细胞和干细胞,EBM™- Plus 内皮细胞生长基础培养基-Plus

Catalog #: CC-5036
EBMTM Plus Endothelial Basal Medium

Product Overview

EBMTM Plus is an endothelial basal medium sold separately. This can be used with 1 kit of CC-4542 (endothelial cell growth medium SingleQuotsTM Supplements and Growth Factors for use with HUVEC cells). The full BulletKitTM consisting of basal medium and supplements can be purchased together using the catalog number CC-5035. Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow CloneticsTM Primary Cells.

EBMTM Plus 是一种内皮基础培养基，单独出售。 这可以与 1 套 CC-4542（内皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子用于 HUVEC 细胞）一起使用。 由基础培养基和补充剂组成的完整 BulletKitTM 可以使用目录号 CC-5035 一起购买。 从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。 每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。 每种类型的内皮细胞生长培养基在用于培养 CloneticsTM 原代细胞时都能保证发挥作用。


The following Endothelial Cell Growth Medium BulletKitsTM are available:

  • EGMTM-2 BulletKitTM – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
  • EGMTMTM BulletKitTM – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
  • EGMTM-Plus BulletKitTM – Enhanced version of EGMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTM Medium.
  • EGMTM-2 MV BulletKitTM – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.
  • EGMTM-MV BulletKitTM – Bovine Microvascular Cells, contains 5% FBS and BBE. Does not contain exogenous VEGF.

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Content & Storage

Content

1 x Bottle Basal Medium 475 mL

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ReagentPack™ Subculture Reagents, 100 mL CC-5034

ReagentPack™ 继代培养试剂，100 mL CC-5034

EBM™- Plus Endothelial Cell Growth Basal Medium-Plus
HUVEC – Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus CC-2935
 HUVEC – EGM™-Plus CC-2935 中不含 VEGF 的人脐静脉内皮细胞，单一供体

lonza CC-4107,原代细胞和干细胞,hEGF-人表皮生长因子

Catalog #: CC-4107
hEGF, Human Epidermal Growth Factor, 3 µg/mL, 0.5 mL

Product Overview

表皮生长因子 (EGF) 是一种通过与其受体 EGFR 结合来刺激细胞生长、增殖和分化的生长因子。 人 EGF 是一种 6045-Da 蛋白质，具有 53 个氨基酸残基和三个分子内二硫键 EGF 导致细胞增殖、分化和存活。

重组表皮生长因子，浓度为 3 µg/ml

Content & Storage

Content

1 x Vial Containing 3 ug/mL hEGF 0.5 mL

lonza CC-3119,原代细胞和干细胞,SABM™ 小气道上皮细胞生长基础培养基

Catalog #: CC-3119
SABMTM Small Airway Epithelial Basal Medium

Product Overview

SABMTM 是一种基础培养基，单独出售。 这被用作完整 SAGMTM 小气道上皮细胞生长培养基 BulletKitTM（目录号 CC-3118）的一个组件，该培养基由包装在一起的基础和补充剂组成。 SAGMTM BulletKitTM 不含血清，经过优化可培养人小气道上皮细胞 (SAEC)。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

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HMEC – Human Mammary Epithelial Cells CC-2551

HMEC – 人乳腺上皮细胞 CC-2551

SABM™ Small Airway Epithelial Cell Growth Basal Medium
B-ALI™ Bronchial Air-Liquid Interface Medium BulletKit™ 00193514

B-ALI™ 支气管气液接口中号 BulletKit™ 00193514

SABM™ Small Airway Epithelial Cell Growth Basal Medium
SAEC – Human Small Airway Epithelial Cells CC-2547
SABM™ Small Airway Epithelial Cell Growth Basal Medium
D-SAEC-As – Diseased Small Airway Epithelial Cells – Asthma CC-2932

D-SAEC-As – 患病的小气道上皮细胞 – 哮喘 CC-2932

SABM™ Small Airway Epithelial Cell Growth Basal Medium
D-SAEC-COPD – Diseased Small Airway Epithelial Cells – COPD CC-2934

D-SAEC-COPD – 患病小气道上皮细胞 – COPD CC-2934

SABM™ Small Airway Epithelial Cell Growth Basal Medium
D-SAEC-CF – Diseased Small Airway Epithelial Cells – Cystic Fibrosis CC-2933

D-SAEC-CF – 患病小气道上皮细胞 – 囊性纤维化 CC-2933

SABM™ Small Airway Epithelial Cell Growth Basal Medium
SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit™ CC-3118

SAGM™ 小气道上皮细胞生长培养基 BulletKit™ CC-3118

Data

SABM™ Small Airway Epithelial Cell Growth Basal Medium

Co-culture Experiments with Lonza’s Small Airway Epithelial Cells and SAGM™ BulletKit™ Media

 数据由 Furukawa 等人提供。 Lonza 的 SAEC 细胞与原代正常乳腺上皮细胞、乳腺癌细胞系 MCF-7 和 MDA-MB-231 在 Lonza 的 SAGM™ BulletKit™ 生长培养基中共培养。 乳腺癌细胞系在存在小气道上皮细胞的情况下表现出形态和增殖率的差异。 上图显示了 MCF-7 细胞系的荧光（红色）覆盖的相差成像。 MCF-7 细胞系在 SAGM™ 培养基中以紧密簇的形式存活。 当与 Lonza 的原代 SAEC 共培养时，MCF-7 细胞在保持聚集的同时表现出增强的生长。

lonza 2W-501,原代细胞和干细胞,NK-人类自然杀伤细胞

Catalog #: 2W-501

Crypreserved ampule of Peripheral Blood Human Natural Killer Cells (CD56 negative immunomagnetic selection) containing ≥ 5 million cells

Product Overview

自然杀伤 (NK) 细胞是免疫系统的淋巴细胞，作为先天性和适应性免疫反应的一部分，对宿主防御和免疫调节至关重要。 它们对免疫治疗研究人员特别感兴趣，因为它们有望专门针对和破坏癌细胞和肿瘤，同时保持健康细胞完好无损。

Cells You Can Count On

自然杀伤细胞的传统特征是存在 CD56 细胞表面标志物且不存在 CD3。 此外，CD16 对 NK 细胞功能很重要，因为 CD16 诱导细胞因子表达和细胞毒效应活性。

使用 CD56 标记的阳性 (2W-502) 或阴性 (2W-501) 免疫磁性选择分离 Lonza NK 细胞。 基于 CD56+ 细胞的存在，纯度≥90%。 阳性和阴性选择的 NK 细胞都保证表达 CD56 和 CD16。 然而，NK 细胞的负免疫磁性分离导致 CD16+ 细胞的富集显着增加（通常 > 总细胞的 80%），而 CD16 的表达随阳性选择的 NK 细胞而变化。

Lonza NK 细胞保证表达 CD56 和 CD16，每瓶含有 ≥500 万个活细胞。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Choices in Cell Type and Tissue Source

CD56和CD16的表达可以极大地影响细胞功能。 此外，选择技术也会影响目标细胞的功能。 这就是为什么 Lonza 提供阳性和阴性选择的 NK 细胞，并表征每批细胞中 CD56 和 CD16 的表达水平。

如果外周血衍生的自然杀伤细胞不是您想要的，我们还提供组织来源选择。 脐带血 MNC 或骨髓 MNC 可以去除谱系标记表达细胞，以分离 NK 细胞。 此外，脐带血 CD34+ 细胞或骨髓 CD34+ 细胞可分化为自然杀伤细胞。

Recommended Media and Reagents

  • LGM-3TM Lymphocyte Growth Medium is recommended for optimal performance of NK cells. Additional cytokines may be necessary depending on application.

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of Human Peripheral Blood NK containing ≥ 5 million cells

Related Products
NK - Human Natural Killer Cells, Cryopreserved, Negative Selection, 5 Million
LGM-3TM Lymphocyte Growth Medium-3 CC-3211

LGM-3TM 淋巴细胞生长培养基-3 CC-3211

NK – Human Natural Killer Cells 2W-502

NK – 人类自然杀伤细胞 2W-502

NK - Human Natural Killer Cells, Cryopreserved, Negative Selection, 5 Million
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702

人外周血单个核细胞 (hPBMC)，冷冻保存，5000 万个 CC-2702

NHDC – Human Dendritic Cells CC-2701
Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 Million 2W-200

人外周血 CD4+ T 细胞，冷冻保存，1000 万个 2W-200

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 100,000 2M-101
Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 300,000 2M-101A

人骨髓 CD34+ 祖细胞，冷冻保存，300,000 2M-101A

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000 2M-101B

人骨髓 CD34+ 祖细胞，冷冻保存，500,000 2M-101B

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 1 Million 2M-101C

人骨髓 CD34+ 祖细胞，冷冻保存，100 万个 2M-101C

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 2 Million 2M-101D

人骨髓 CD34+ 祖细胞，冷冻保存，200 万个 2M-101D

Human Bone Marrow MNCs, Cryopreserved, 5 Million 2S-101D

人骨髓 MNC，冷冻保存，500 万个 2S-101D

Human Bone Marrow Mononuclear Cells (MNCs) (300 Million Cells) 2M-125E

人骨髓单个核细胞 (MNC)（3 亿个细胞）2M-125E

NK - Human Natural Killer Cells, Cryopreserved, Negative Selection, 5 Million
Unprocessed Human Bone Marrow, Fresh, 10 mL 1M-105

未加工的人骨髓，新鲜，10 mL 1M-105

NK - Human Natural Killer Cells, Cryopreserved, Negative Selection, 5 Million
Unprocessed Human Bone Marrow, Fresh, 25 mL 1M-125

未加工的人骨髓，新鲜，25 mL 1M-125

NK - Human Natural Killer Cells, Cryopreserved, Negative Selection, 5 Million
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza 00195409,原代细胞和干细胞,RtEGM™ 视网膜色素上皮细胞生长培养基 BulletKit™

Catalog #: 00195409

Culture system containing RtEBMTM Basal Medium (00195406) and RtEGMTM SingleQuotsTM Supplements (00195407) required for growth of Retinal Pigment Epithelial Cells

含有视网膜色素上皮细胞生长所需的 RtEBMTM 基础培养基 (00195406) 和 RtEGMTM SingleQuotsTM 补充剂 (00195407) 的培养系统

Product Overview

RtEGMTM 视网膜色素上皮细胞生长培养基针对生长的人类 RPE 细胞进行了优化和性能测试。 它含有2％的血清。

Applications

  • Age-related macular degeneration
  • Retinitis pigmentosa
  • Diabetic retinopathy
  • Photoreceptor degeneration
  • Gene expression
  • Cellular interactions

Content & Storage

Content

1 x RtEBMTM Basal Medium (00195406), 200 mL

1 x RtEGMTM SingleQuotsTM Supplement Pack (00195407) containing:

1 x Bottle L-Glutamine, 4.00 mL
1 x Gray Cap Vial with hFGF-B, 1.00 mL
1 x Bottle FBS, 4.00 mL
1 x Red Cap Vial with GA-1000, 0.20 mL

 

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RtEGM™ Retinal Pigment Epithelial Cell Growth Medium BulletKit™
H-RPE – Human Retinal Pigment Epithelial Cells 00194987

H-RPE – 人视网膜色素上皮细胞 00194987

RtEGM™ Retinal Pigment Epithelial Cell Growth Medium BulletKit™
RtEBM™ Retinal Epithelial Cell Basal Medium 00195406

RtEBM™ 视网膜上皮细胞基础培养基 00195406

RtEGM™ Retinal Pigment Epithelial Cell Growth Medium BulletKit™
RtEGM™ Retinal Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors 00195407
RtEGM™ 视网膜上皮细胞生长培养基 SingleQuots™ 添加剂和生长因子 00195407

lonza CC-2512B,原代细胞和干细胞,肺成纤维细胞培养试剂盒

Catalog #: CC-2512B
Lung Fibroblast Cell Culture Kit
肺成纤维细胞培养试剂盒

Product Overview

购买我们的肺成纤维细胞培养试剂盒并获得细胞和培养基的折扣价。该试剂盒包括一个冷冻保存的肺成纤维细胞安瓿（货号 CC-2512）和 FGMTM-2 BulletKitTM 成纤维细胞生长培养基（货号 CC- 第3132章

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial
1 x Bottle Basal Medium 500 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Gray Cap Vial with hFGF-B 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Bottle FBS 10.00 mL

lonza CC-2555,原代细胞和干细胞,PrEC-人类前列腺上皮细胞

Catalog #: CC-2555

Cryopreserved ampule of Prostate Epithelial Cells (PrEC) containing ≥500,000 cells

含有≥500,000 个细胞的冷冻保存的前列腺上皮细胞 (PrEC) 安瓿

Product Overview

Prostate cells provide a glandular function in the body by generating fluid which serves several functions in reproduction. Lonza’s Human Prostate Cells are available from epithelial, smooth muscle, and stromal tissue. Each cell type has an optimized growth media for best results in culture.

Prostate Epithelial Cells (PrEC) are guaranteed through 15 population doublings and stain positive for cytokeratin (clone 8.13). Cryopreserved PrEC are shipped in first or second passage.

All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
前列腺细胞通过产生在生殖中具有多种功能的液体来提供体内的腺体功能。 Lonza 的人类前列腺细胞可从上皮、平滑肌和基质组织中获得。 每种细胞类型都有优化的生长培养基，以获得最佳培养效果。

前列腺上皮细胞 (PrEC) 通过 15 次群体倍增得到保证，并且细胞角蛋白染色呈阳性（克隆 8.13）。 冷冻保存的 PrEC 在第一次或第二次运输中运输。

所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。

Benefits

  • Guaranteed through 15 population doublings
  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

Applications

  • Benign prostatic hyperplasia (BPH)
  • Prostitis
  • Prostate cancer
  • Procreation research
  • Drug discovery
  • Male prostate physiology

Content & Storage

Content

1 x Cryopreserved ampule PrEC containing ≥500,000 cells

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PrEC – Human Prostate Epithelial Cells
PrSMC – Human Prostate Smooth Muscle Cells CC-2587

PrSMC – 人前列腺平滑肌细胞 CC-2587

PrEC – Human Prostate Epithelial Cells
PrSC – Human Prostate Stromal Cells CC-2508

PrSC – 人类前列腺基质细胞 CC-2508

PrEC – Human Prostate Epithelial Cells
PrEGM™ Prostate Epithelial Cell Growth Medium BulletKit™ CC-3166

PrEGM™ 前列腺上皮细胞生长培养基 BulletKit™ CC-3166

PrEC – Human Prostate Epithelial Cells
P2 Primary Cell 4D-Nucleofector™ X Kit L V4XP-2012

P2 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-2012

PrEC – Human Prostate Epithelial Cells
P2 Primary Cell 4D-Nucleofector™ X Kit L V4XP-2024

P2 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-2024

PrEC – Human Prostate Epithelial Cells
P2 Primary Cell 4D-Nucleofector™ X Kit S V4XP-2032

P2 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-2032

PrEC – Human Prostate Epithelial Cells
P2 Primary Cell 96-well Nucleofector™ Kit V4SP-2096
PrEC – Human Prostate Epithelial Cells
P2 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-2960

P2 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-2960

PrEC – Human Prostate Epithelial Cells
P2 Primary Cell 384-well Nucleofector™ Kit V5SP-2002

P2 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-2002

PrEC – Human Prostate Epithelial Cells
P2 Primary Cell 384-well Nucleofector™ Kit V5SP-2010
P2 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-2010

lonza CC-3232,原代细胞和干细胞,用于间充质干细胞的胰蛋白酶/EDTA

Catalog #: CC-3232
Trypsin/EDTA for MSC, 100 mL

Product Overview

Trypsin/EDTA (catalog no. CC-3232): is Lonza’s recommended Trypsin/EDTA for all strongly adherent primary cells including normal human articular chondrocytes (NHAC-Kn), human bone marrow derived mesenchymal stem cells (hMSC), and dental pulp stem cells (DPSC).

胰蛋白酶/EDTA（目录号 CC-3232）：是 Lonza 推荐的用于所有强粘附原代细胞的胰蛋白酶/EDTA，包括正常人关节软骨细胞 (NHAC-Kn)、人骨髓间充质干细胞 (hMSC) 和牙髓干细胞 细胞（DPSC）。

lonza CC-3216,原代细胞和干细胞,CGM™ 软骨细胞生长培养基 BulletKit™

Catalog #: CC-3216
CGMTM Chondrocyte Growth BulletKitTM

Product Overview

Articular cartilage is characterized by high wear resistance and poor regenerative qualities. It is responsible for much of the compressive resistance and load bearing qualities of the knee joint.

  • CGMTM Chondrocyte Growth Medium is specially designed for expansion of chondrocytes. The BulletKitTM is conveniently packaged with basal medium and supplements included for the growth of chondrocytes.
  • CDMTM Chondrocyte Differentiation Medium is also available and sold separately for differentiation of human chondrocytes

Benefits

Applications:

  • Osteoarthritis
  • Cartilage failure
  • Cellular matrix linkage rupture
  • Chondrocyte protein synthesis inhibition
  • Chondrocyte apoptosis

Content & Storage

Content

1 x Bottle Basal Medium 500 mL
1 x Bottle FBS 25.00 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Yellow Cap Vial with R3-IGF 1.00 mL
1 x Bottle bFGF 2.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL
1 x Lilac Cap Vial with Insulin 1.00 mL

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CGM™ Chondrocyte Growth Medium BulletKit™
CDM™ Chondrocyte Differentiation Medium BulletKit™ CC-3225

CDM™ 软骨细胞分化培养基 BulletKit™ CC-3225

CGM™ Chondrocyte Growth Medium BulletKit™
NHAC-kn – Human Knee Articular Chondrocytes CC-2550

NHAC-kn – 人膝关节软骨细胞 CC-2550

CGM™ Chondrocyte Growth Medium BulletKit™
CBM™ Chondrocyte Growth Basal Medium CC-3217

CBM™ 软骨细胞生长基础培养基 CC-3217

CGM™ Chondrocyte Growth Medium BulletKit™
CGM™ Chondrocyte Growth Medium SingleQuots™ Supplements and Growth Factors CC-4409
CGM™ 软骨细胞生长培养基 SingleQuots™ 补充剂和生长因子 CC-4409

lonza CC-7049,原代细胞和干细胞,HPdLF-人牙周膜成纤维细胞

Catalog #: CC-7049
HPdLF, Periodontal Ligament Fibroblasts

Product Overview

人牙周韧带成纤维细胞 (HPdLF) 从将臼齿固定到颌骨的韧带中分离出来。 保证人牙周膜成纤维细胞通过 10 倍种群倍增和泛细胞角蛋白染色阴性。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

lonza 2M-302,原代细胞和干细胞,人骨髓基质细胞，500 万

Catalog #: 2M-302
Cryopreserved ampule of Human Bone Marrow Stromal Cells containing ≥5 million cells

Product Overview

当骨髓细胞在特定条件下进行培养时，会形成粘附细胞的基质层。基质层由许多细胞类型组成，包括成纤维细胞、间充质干细胞、脂肪细胞、内皮细胞和巨噬细胞。这些基质细胞充当造血祖细胞和干细胞的饲养层，允许祖细胞和干细胞在不添加外源细胞因子的情况下在这些培养物中继续增殖和分化数周。

这种长期培养系统使研究人员能够研究骨髓微环境的体外模型，包括细胞间相互作用、粘附分子和细胞因子分泌。这些和许多其他因素允许对血细胞产生、祖细胞定型和分化、干细胞更新和 LTC-IC 测定进行严格调节。仅提供非辐照。

并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

推荐培养基：来自 StemCell Technologies, Inc. 的 MyeloCultTM H5100。

骨髓基质细胞经常与骨髓间充质干细胞混淆。如果您需要具有自我更新能力和分化多个谱系（包括成骨谱系、脂肪生成谱系和软骨生成谱系）的纯骨髓衍生多能干细胞群，请访问骨髓间充质干细胞页面。

只有在专门使用适当的培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis (CoA) is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of Human Bone Marrow Non-Irradiated Stromal Cells containing ≥ 5 million cells

lonza R-CX-500,原代细胞和干细胞,大鼠脑皮层神经元

Catalog #: R-CX-500

Cryopreserved ampule of Rat Brain Cortex Neurons containing ≥4 million cells

Product Overview

即用型 CloneticsTM 大鼠脑皮层 来自大鼠 (E18, 19) 脑的神经元是通过标准化方法制备的高质量原代胚胎脑神经元细胞（包括神经胶质细胞）的细胞悬液，可立即进行培养。

每瓶皮质细胞含有大约 400 万个活细胞。 细胞将使用推荐的接种密度和培养基接种到 24 孔板的 10 个或更多孔和 96 孔板的 50 个或更多孔中。接种后的最初几天会发生细胞死亡，并观察到碎片 . 这很正常。 培养约 4 天后，细胞将形成神经突网络，到第 7 天，碎片将最少。

Recommended Medium: PNGMTM BulletKitTM (CC-4461)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

 

Content & Storage

Content

1 x Cryopreserved ampule of Rat Cortex Neurons containing ≥ 4,000,000 cells

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Rat Brain Cortex Neurons
PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461

PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

Rat Brain Cortex Neurons
Rat Cerebellar Granular Neurons R-CB-503

大鼠小脑颗粒神经元 R-CB-503

Rat Brain Cortex Neurons
Rat Brain Striatum Neurons R-CP-502
Rat Brain Cortex Neurons
Rat Neonatal Dorsal Root Ganglion (DRG) Neurons R-DRG-505
Rat Brain Cortex Neurons
Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons R-EDRG-515

大鼠胚胎背根神经节 (eDRG) 神经元 R-EDRG-515

Rat Brain Cortex Neurons
Rat Brain Hippocampus Neurons R-HI-501
Rat Brain Cortex Neurons
Rat Brain Hypothalamus Neurons R-HTH-507

大鼠脑下丘脑神经元 R-HTH-507

Rat Brain Cortex Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
Rat Brain Cortex Neurons
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024

P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024

Rat Brain Cortex Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032
Rat Brain Cortex Neurons
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096
Rat Brain Cortex Neurons
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

Rat Brain Cortex Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002
Rat Brain Cortex Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza CC-2576,原代细胞和干细胞,BSMC-人支气管平滑肌细胞

Catalog #: CC-2576

Cryopreserved ampule of Bronchial Smooth Muscle Cells (BSMC) containing ≥ 500,000 cells

Product Overview

人支气管平滑肌细胞 (BMSC) 是从正常组织的主要支气管中分离出来的。 细胞在第 2 代中运输。

患病的人支气管平滑肌细胞 (D-BSMC) 也可从被诊断患有哮喘、COPD 和囊性纤维化的捐赠者处获得。 这些细胞的详细捐赠者信息可以通过联系我们的科学支持团队获得。

冷冻保存的 CloneticsTM 人支气管平滑肌细胞通过 15 次细胞倍增得到保证，α 平滑肌肌动蛋白染色呈阳性，von Willebrand 因子 VIII 呈阴性。所有细胞的支原体、细菌、酵母和真菌检测均为阴性。HIV-1、乙型肝炎和 并非所有供体和/或细胞批次都检测到丙型肝炎。 为购买的每个细胞批次提供分析证书。

Benefits

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

Content & Storage

Content

1 x Cryopreserved ampule BSMC containing ≥ 500,000 cells/vial

Related Products
BSMC – Human Bronchial Smooth Muscle Cells
SAEC – Small Airway Epithelial Cells for S-ALITM Small Airway Air-Liquid-Interface Medium CC-2547S

SAEC – 用于 S-ALITM 小气道气液界面培养基 CC-2547S 的小气道上皮细胞

BSMC – Human Bronchial Smooth Muscle Cells
SAEC – Human Small Airway Epithelial Cells CC-2547

SAEC – 人类小气道上皮细胞 CC-2547

BSMC – Human Bronchial Smooth Muscle Cells
HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527

HMVEC-L – 人肺微血管内皮细胞 CC-2527

BSMC – Human Bronchial Smooth Muscle Cells
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012
P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1012
BSMC – Human Bronchial Smooth Muscle Cells
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1024

BSMC – Human Bronchial Smooth Muscle Cells
P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032

P1 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-1032

BSMC – Human Bronchial Smooth Muscle Cells
P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-1096

BSMC – Human Bronchial Smooth Muscle Cells
P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960
BSMC – Human Bronchial Smooth Muscle Cells
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002
BSMC – Human Bronchial Smooth Muscle Cells
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010
P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1010

lonza HUCSD,原代细胞和干细胞,人肝细胞，冷冻保存的单一供体，悬浮液

Catalog #: HUCSD

冷冻保存的单一供体人肝细胞的特点是在短期悬浮代谢测定中具有高活力和一般的 I 期和 II 期代谢活性。 每个安瓿包含 > 500 万个细胞。

Product Overview

冷冻保存的单一供体人肝细胞的特点是在短期悬浮代谢测定中具有高活力和一般的 I 期和 II 期代谢活性。 悬浮合格的肝细胞非常适合短期（<4 小时）体外药物清除试验。 悬浮合格的肝细胞不适合需要在粘附的单层中培养肝细胞的应用。

Benefits

  • Precharacterized for general metabolism
  • Certificate of Analysis (CoA) provided for every cell lot

Applications

  • Suspension metabolism studies

Content & Storage

Content

1 x Cryopreserved ampule Human Hepatocytes Suspension, Single Donor, containing >5 million viable cells

Related Products
Human Hepatocytes, Cryopreserved Single Donor , Suspension
Human Hepatocytes, Cryopreserved, Plateable and Interaction Qualified HUCPI

人肝细胞，冷冻保存，可电镀和相互作用合格的 HUCPI

Human Hepatocytes, Cryopreserved Single Donor , Suspension
Human Hepatocytes, Cryopreserved, Plateable HUCPG
人肝细胞，冷冻保存，可电镀 HUCPG

lonza,原代细胞和干细胞,FGM™ 成纤维细胞生长培养基 BulletKit™

Catalog #: CC-3130

停产通知：该产品即将停产。 当前库存将被出售，直到库存耗尽。 请使用产品 CC-3132 作为替代品。

用于人成纤维细胞培养的成纤维细胞生长培养基 BulletKitTM

Product Overview

FGMTM Fibroblast Growth Medium was developed to support growth of fibroblasts.

Content & Storage

Content

1 x FBMTM Basal Medium (CC-3131), 500 mL

1 x FGM SingleQuotsTM Supplement Pack (CC-4134) containing:

1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Gray Cap Vial with hFGF-B, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL

lonza,lonza HUCNP,lonza HUCLS,原代细胞和干细胞,人肝混合非实质细胞

Catalog #: HUCNP
Human cryopreserved liver mixed NPCs cells
人冷冻保存的肝脏混合 NPCs 细胞

Product Overview

Human Cryopreserve Liver Mixed NPCs are a native mixture of Non-parenchymal cells from human liver. These are supplied in > 1million cells/vial and can have a varying amount of the different non-parenchymal cells.

This product is most suitable for liver cell co-culture applications

人冷冻保存肝脏混合 NPC 是来自人肝脏的非实质细胞的天然混合物。 这些以 > 100 万个细胞/小瓶的形式提供，并且可以具有不同数量的不同非实质细胞。

本产品最适合肝细胞共培养应用

Benefits

  • Native cell ratios,
  • Never passaged for close to in vivo function

Applications

ADMETox, Liver Disease, NASH

Content & Storage

Content

Cryo Vial

Related Products

Liver Mixed Non-parenchymal Cells, Human
Stellate cells, human cryopreserved, passage 1 HUCLS
星状细胞，人类冷冻保存，第 1 代 HUCLS

lonza,lonza 2M-125C,原代细胞和干细胞,人骨髓单个核细胞 MNC

货号: 2M-125C

Cryopreserved ampule of Human Bone Marrow Mononuclear Cells containing ≥25 million cells

含有≥2500万个细胞的人骨髓单个核细胞的冷冻保存安瓿

Product Overview

Bone marrow mononuclear cells (common abbreviations: BMNC, BM MNC, and BM-MNC) are a mixed population of single nucleus cells including monocytes, lymphocytes, and hematopoietic stem and progenitor cells.

骨髓单个核细胞（常用缩写：BMNC、BM MNC 和 BM-MNC）是单核细胞的混合群体，包括单核细胞、淋巴细胞以及造血干细胞和祖细胞。

BMNCs You Can Count on to Perform

Cryopreserved Lonza Bone Marrow Mononuclear Cells are isolated from whole bone marrow via density gradient separation. Isolating mononuclear cells eliminates erythrocytes and graulocytes, producing a more stable cell product. Lonza BMNCs are available fresh or frozen from a large and varied donor pool. They are guaranteed to contain either ≥5, 25, 100, 200 and 300 million viable cells per ampoule, depending upon product purchased. Fresh cells are suspended in HBSS with 5 mM EDTA and 0.5% BSA and are available in a variety of cells per vial packaging sizes. Fresh BMNC orders require a minimum order quantity of two vials per donor. Please contact Customer Service for information on sizes and pricing for fresh cells.

通过密度梯度分离从全骨髓中分离冷冻保存的龙沙骨髓单核细胞。 分离单核细胞消除红细胞和粒细胞，产生更稳定的细胞产物。 Lonza BMNCs 可从大量多样的供体库中获得新鲜或冷冻。 根据购买的产品，它们保证每个安瓿含有≥5、25、100、200 和 3 亿个活细胞。 新鲜细胞悬浮在含有 5 mM EDTA 和 0.5% BSA 的 HBSS 中，每瓶包装尺寸有多种细胞可供选择。 新鲜的 BMNC 订单要求每个捐赠者至少订购两瓶。 有关新鲜细胞的尺寸和价格信息，请联系客户服务。

Choices in Cell Type and Tissue Source

Lonza offers choices in cell type and tissue source so you can choose the right product for your research and budget needs. Depending upon your ultimate cell of choice, you can choose from unprocessed bone marrow, bone marrow MNCs, bone marrow CD34+ cells, bone marrow stromal cells (for use as feeder cells), or bone marrow mesenchymal stem cells. In addition, if bone marrow is not your preferred tissue source, we also offer cord blood derived cells.

Lonza 提供细胞类型和组织来源的选择，因此您可以选择适合您的研究和预算需求的产品。 根据您选择的最终细胞，您可以选择未加工的骨髓、骨髓 MNC、骨髓 CD34+ 细胞、骨髓基质细胞（用作饲养细胞）或骨髓间充质干细胞。 此外，如果骨髓不是您首选的组织来源，我们还提供脐带血来源的细胞。

Supported by an Optimized Culture System

HPGMTM Hematopoietic Growth Medium is recommended for optimal performance in serum-free culturing (additional cytokines may be necessary depending on application). Alternative media containing serum may also be acceptable. Please contact Scientific Support for serum containing media recommendations.

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

建议使用 HPGMTM 造血生长培养基以在无血清培养中获得最佳性能（根据应用，可能需要额外的细胞因子）。 含有血清的替代培养基也是可以接受的。 有关含血清培养基的建议，请联系科学支持。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。 如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased
  • 支原体、细菌、酵母菌和真菌检测呈阴性
    并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎
    为购买的每个细胞批次提供分析证书

Content & Storage

Content

1 x Cryopreserved ampule BMNCs containing ≥ 25 million cells

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English

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Human Bone Marrow Stromal Cells, 5 Million 2M-302
人骨髓基质细胞，500 万个 2M-302
Human Bone Marrow Mononuclear Cells MNCs, Cryopreserved, 25 Million Cells
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702
人外周血单个核细胞 (hPBMC)，冷冻保存，5000 万个 CC-2702
Human Bone Marrow Mononuclear Cells MNCs, Cryopreserved, 25 Million Cells
hMSC – Human Mesenchymal Stem Cells PT-2501
hMSC – 人间充质干细胞 PT-2501
Human Bone Marrow Mononuclear Cells MNCs, Cryopreserved, 25 Million Cells
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza,lonza 2W-502,lonza CC-3211,原代细胞和干细胞,NK-人类自然杀伤细胞

货号: 2W-502
Human Natural Killer Cells pos selection, ≥5 million cells
人自然杀伤细胞 pos 选择，≥500 万个细胞

Product Overview

Natural Killer (NK) cells are lymphocytes of the immune system that are critical in host defense and immune regulation as part of both the innate and adaptive immune response. They are of particular interest to immunotherapy researchers as they hold the promise to specifically target and destroy cancer cells and tumors while leaving healthy cells intact. Cells You Can Count on to Perform Natural Killer cells are traditionally characterized by the presence of the CD56 cell surface marker and absence of CD3. In addition, CD16 is important for NK cell function because CD16 induces cytokine expression and cytotoxic effector activity. Lonza NK cells are isolated using positive (2W-502) or negative (2W-501) immunomagnetic selection for the CD56 marker. Purity is ≥90% based on the presence of CD56+ cells. Both positively and negatively selected NK cells are guaranteed to express CD56 and CD16. However, negative immunomagnetic separation of NK cells results in significantly greater enrichment of CD16+ cells (typically > 80% of total cells), whereas expression of CD16 is variable with the positively selected NK cells. Lonza NK cells are guaranteed to express CD56 and CD16, and contain ≥5 million viable cells per vial. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Choices in Cell Type and Tissue Source Expression of CD56 and CD16 can greatly influence cell function. In addition, selection techniques can also influence function of your target cells. That’s why Lonza offers both positively and negatively selected NK cells and characterizes the expression levels of both CD56 and CD16 in every lot of cells. We also offer choices in tissue source if peripheral blood derived natural killer cells aren’t what you’re looking for. Cord blood MNCs or bone marrow MNCs can be depleted of lineage marker expressing cells in order to isolate NK cells. In addition, cord blood CD34+ cells or bone marrow CD34+ cells can be differentiated into natural killer cells. Supported by Lonza Medium LGM-3TM Lymphocyte Growth Medium is recommended for optimal performance of NK cells. Additional cytokines may be necessary depending on application. Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

自然杀伤 (NK) 细胞是免疫系统的淋巴细胞，作为先天性和适应性免疫反应的一部分，对宿主防御和免疫调节至关重要。它们对免疫治疗研究人员特别感兴趣，因为它们有望专门针对和破坏癌细胞和肿瘤，同时保持健康细胞完好无损。您可以依靠的细胞来执行自然杀伤细胞的传统特征是存在 CD56 细胞表面标志物且不存在 CD3。此外，CD16 对 NK 细胞功能很重要，因为 CD16 诱导细胞因子表达和细胞毒效应活性。使用 CD56 标记的​​阳性 (2W-502) 或阴性 (2W-501) 免疫磁性选择分离 Lonza NK 细胞。基于 CD56+ 细胞的存在，纯度≥90%。阳性和阴性选择的 NK 细胞都保证表达 CD56 和 CD16。然而，NK 细胞的负免疫磁性分离导致 CD16+ 细胞的富集显着增加（通常 > 总细胞的 80%），而 CD16 的表达随阳性选择的 NK 细胞而变化。 Lonza NK 细胞保证表达 CD56 和 CD16，每瓶含有 ≥500 万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。 CD56和CD16的细胞类型和组织来源表达的选择可以极大地影响细胞功能。此外，选择技术也会影响目标细胞的功能。这就是为什么 Lonza 提供阳性和阴性选择的 NK 细胞，并表征每批细胞中 CD56 和 CD16 的表达水平。如果外周血衍生的自然杀伤细胞不是您想要的，我们还提供组织来源选择。脐带血 MNC 或骨髓 MNC 可以去除谱系标记表达细胞，以分离 NK 细胞。此外，脐带血 CD34+ 细胞或骨髓 CD34+ 细胞可分化为自然杀伤细胞。由 Lonza 培养基支持 LGM-3TM 淋巴细胞生长培养基推荐用于 NK 细胞的最佳性能。根据应用，可能需要额外的细胞因子。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Content & Storage

Content

5 million cells/vial

Instructions, SDS, CoA

NK – Human Natural Killer Cells

Safety Data Sheets (SDS)

View SDS

SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Related Products

NK – Human Natural Killer Cells
LGM-3TM Lymphocyte Growth Medium-3 CC-3211
 LGM-3TM 淋巴细胞生长培养基-3 CC-3211

lonza,lonza 2W-400A,CC-2701,原代细胞和干细胞,人外周血 CD14+ 单核细胞

Catalog #: 2W-400A

Cryopreserved ampule of Human Peripheral Blood CD14+ Monocytes, positive selection, containing ≥40 million cells

人外周血 CD14+ 单核细胞冻存安瓿，阳性选择，含有≥4000 万个细胞

Product Overview

Monocytes are found in the circulating peripheral blood where they make up 10-20% of the total mononuclear cell population. Monocytes are characterized by their phenotypic expression of CD14, and thus, are commonly called CD14+ monocytes. They play an important role in host defense as circulating monocytes and differentiation into tissue macrophages, and can differentiate into dendritic cells with potent antigen-presenting capability.

单核细胞存在于循环外周血中，占总单核细胞群的 10-20%。 单核细胞的特征在于它们的 CD14 表型表达，因此，通常称为 CD14+ 单核细胞。 它们作为循环单核细胞和分化成组织巨噬细胞在宿主防御中发挥重要作用，并且可以分化成具有强大抗原呈递能力的树突状细胞。

Cells You Can Count on to Perform

Lonza Human Peripheral Blood CD14+ Monocytes are isolated from peripheral blood of screened, healthy, untreated (non-mobilized peripheral blood) donors. Briefly, mononuclear cells (MNCs) are isolated from apheresis derived peripheral blood. The MNC population is then further purified via positive immunomagnetic selection directed against CD14. Cryopreserved CD14+ Monocytes are guaranteed to express CD14 and will contain either ≥10, 20, or 40 million viable cells per ampoule depending upon the product ordered. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Lonza Human Peripheral Blood CD14+ 单核细胞是从经过筛选的、健康的、未经治疗的（未动员的外周血）供体的外周血中分离出来的。 简而言之，单核细胞 (MNC) 是从单采术衍生的外周血中分离出来的。 然后通过针对 CD14 的阳性免疫磁性选择进一步纯化 MNC 群体。 冷冻保存的 CD14+ 单核细胞保证表达 CD14，并且根据订购的产品，每个安瓿将包含 ≥10、20 或 4000 万个活细胞。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Choices in Cell Type and Tissue Source

Hematopoietic and immune research requires a variety of donors and cell types. That’s why Lonza offers many different peripheral blood cell types from a variety of donors, including PBMCs and dendritic cells, among others. In addition, if you are interested in exploring the differences between monocytes in different maturation states or from different tissues, we offer bone marrow mononuclear cells.

造血和免疫研究需要多种供体和细胞类型。 这就是为什么 Lonza 提供来自各种供体的多种不同外周血细胞类型，包括 PBMC 和树突状细胞等。 此外，如果您有兴趣探索不同成熟状态或来自不同组织的单核细胞之间的差异，我们提供骨髓单核细胞。

Supported by Lonza Media

The recommended medium varies by application (see below). Please contact Scientific Support for additional culturing recommendations. Media recommendations for macrophage and dendritic cell differentiation are recommendations only.

  • Monocyte Phenotype: RPMI + 10% Fetal Bovine Serum + 2 mM L-glutamine + 10 ng/ml rHU M-CSF (optional)
  • Macrophages: RPMI +10% Fetal Bovine Serum + 2 mM L-glutamine + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids + 25 ng/ml rHU M-CSF
  • Dendritic Cells: LGM-3TM Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4

Lonza CD14+ monocytes are not routinely quality control tested for macrophage or dendritic cell differentiation and differentiation is not guaranteed under the cell warranty. Additional factors including extracellular matrixes or culturing conditions may be required to produce differentiation.

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

Lonza CD14+ 单核细胞没有针对巨噬细胞或树突状细胞分化进行常规质量控制测试，并且细胞保修不保证分化。 产生分化可能需要其他因素，包括细胞外基质或培养条件。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。 如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

 

Content & Storage

Content

1 x Cryopreserved ampule of CD14+ Monocytes containing ≥ 40 million cells

Instructions, SDS, CoA

 

 

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P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012

P3 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-3012

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 40 million
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024
Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 40 million
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032
Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 40 million
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-3096

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 40 million
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 40 million
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 40 million
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 40 million
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702
人外周血单个核细胞 (hPBMC)，冷冻保存，5000 万个 CC-2702
Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 40 million
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218

MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 40 million

Data

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 40 million

Human CD14+ Monocytes

Human CD14+ monocytes differentiating to macrophages

lonza,lonza CC-2554,原代细胞和干细胞,HRCE – 人肾皮质上皮细胞

货号: CC-2554

冷冻保存的肾皮质上皮细胞 (HRCE) 安瓿，含有 ≥500,000 个细胞，Cryopreserved ampule of Renal Cortical Epithelial Cells (HRCE) containing ≥500,000 cells

Product Overview

Lonza’s Human Renal Cells include cells isolated from various normal human kidney tissue specific to each cell type.  Human Renal Proximal Tubule Cells are isolated from the proximal tubule and are ideal for drug transporter studies. Human Renal Epithelial Cells (HRE) are comprised of cells from the cortex and glomerular region. Human Renal Cortical Cells (HRCE) are from proximal and distal tubules. Human Mesangial Cells (HMC) are isolated from the renal glomerulus and modified smooth muscle cells between capillaries.

Renal epithelial cells display a polarized morphology, which is essential for their function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. Physiological processes like osmoregulation and excretion can be studied with renal epithelial cells in vitro.  Human renal epithelial cells are also capable of internalizing bacteria and are located near injury sites in a variety of congenital, metabolic and inflammatory diseases.

Lonza 的人肾细胞包括从对每种细胞类型特异的各种正常人肾组织中分离出来的细胞。人肾近端小管细胞从近端小管中分离出来，是药物转运蛋白研究的理想选择。人肾上皮细胞 (HRE) 由来自皮质和肾小球区域的细胞组成。人肾皮质细胞 (HRCE) 来自近端和远端小管。人系膜细胞 (HMC) 从肾小球和毛细血管之间的修饰平滑肌细胞中分离出来。

肾上皮细胞表现出极化形态，这对其功能至关重要。它们重新吸收肾小球滤液中几乎所有的葡萄糖和氨基酸，同时允许其他没有营养价值的物质从尿液中排出。可以在体外用肾上皮细胞研究渗透调节和排泄等生理过程。人肾上皮细胞也能够内化细菌，并且位于各种先天性、代谢和炎症性疾病的损伤部位附近。

Benefits

  • Guaranteed through 15 population doublings
  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased
  • 通过 15 次人口倍增保证
    支原体、细菌、酵母菌和真菌检测呈阴性
    并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎
    为购买的每个细胞批次提供分析证书

Content & Storage

Content

1 x Cryopreserved ampule of HRCE containing ≥500,000 cells

Instructions, SDS, CoA
Related Products
HRCE – Human Renal Cortical Epithelial Cells
H-RPE – Human Retinal Pigment Epithelial Cells 00194987

H-RPE – 人视网膜色素上皮细胞 00194987

HRCE – Human Renal Cortical Epithelial Cells
InEpC – Human Intestinal Epithelial Cells CC-2931
InEpC – 人肠上皮细胞 CC-2931
HRCE – Human Renal Cortical Epithelial Cells
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540

NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞

HRCE – Human Renal Cortical Epithelial Cells
REGM™ Renal Epithelial Cell Growth Medium BulletKit™ CC-3190
 REGM™ 肾上皮细胞生长培养基 BulletKit™ CC-3190

lonza,lonza MBCP01,原代细胞和干细胞,小鼠 C57BL/6 肝细胞

Catalog #: MBCP01

Cryopreserved ampule of C57BL/6 Mouse Hepatocytes, in a plateable format, containing > 5 million cells

冷冻保存的 C57BL/6 小鼠肝细胞安瓿，可电镀，含有 > 500 万个细胞

Product Overview

Cryopreserved C57BL/6 Mouse Hepatocytes, Plateable are specifically pre tested for metabolic activity of general Phase I and Phase II metabolism in suspension.

They are tested for healthy morphology lasting 72 hours in monlayer sandwich culture.

冷冻保存的 C57BL/6 小鼠肝细胞，可铺板专门针对悬浮液中一般 I 期和 II 期代谢的代谢活性进行了预测试。

它们在单层夹层培养中经过 72 小时的健康形态测试。

Applications

  • Drug metabolism in plated monolayer format
  • Species-species comparisons
  • Genomics

Content & Storage

Content

1 x Cryopreserved ampule of C57BL/6 Mouse Hepatocytes (plateable format) containing > 5 million viable cells

Instructions, SDS, CoA

Related Products
Mouse C57BL/6 Hepatocytes, Cryopreserved, Plateable
Human Hepatocytes, Cryopreserved, Plateable HUCPG

人肝细胞，冷冻保存，可电镀 HUCPG

Mouse C57BL/6 Hepatocytes, Cryopreserved, Plateable
Human Hepatocytes, Cryopreserved, Plateable and Interaction Qualified HUCPI

人肝细胞，冷冻保存，可电镀和相互作用合格的 HUCPI

lonza,lonza CC-4098,lonza CC-3121,原代细胞和干细胞,Bovine Brain Extract (BBE)

货号: CC-4098

Bovine Brain Extract contains endothelial cell proliferation growth factors, 9 mg/mL, 5 mL

牛脑提取物含有内皮细胞增殖生长因子，9 mg/mL，5 mL

Product Overview

Bovine Brain Extract is useful in the culture of various endothelial cell types. The product is offered at a concentration of 9 mg/mL.

牛脑提取物可用于培养各种内皮细胞类型。 该产品的浓度为 9 mg/mL。

Content & Storage

Content

1 x Bottle containing 9 mg/mL Bovine Brain Extract, 5 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

 

 

Related Products
Bovine Brain Extract (BBE), 5 ml
EBM™ Endothelial Cell Growth Basal Medium, 500 mL CC-3121

EBM™ 内皮细胞生长基础培养基，500 mL CC-3121

Bovine Brain Extract (BBE), 5 ml
Bovine Brain Extract (BBE), 2 ml CC-4092
 牛脑提取物 (BBE)，2 ml CC-4092

lonza,lonza CC-2902,原代细胞和干细胞,InMyoFib-人类肠道肌成纤维细胞

货号: CC-2902

Cryopreserved ampule of Human Intestinal Myofibroblasts (InMyoFib) containing ≥500,000 cells

Help us improve our primary cell offering. Invest 5 minutes to share your specific needs with us. Fill out our survey and enter the chance to win an amazon voucher.

含有≥500,000 个细胞的人肠道肌成纤维细胞 (InMyoFib) 的冷冻保存安瓿

帮助我们改进我们的主要细胞产品。 花 5 分钟时间与我们分享您的特定需求。 填写我们的调查表并有机会赢取亚马逊代金券。

Product Overview

Lonza offers Human Intestinal Myofibroblasts (H-InMyoFib), cryopreserved at passage 2 with ≥500,000 cells per vial. H-InMyoFibs share phenotypic characteristics of both fibroblasts and smooth muscle cells but do not express smooth muscle markers such as smoothelin, caldesmon, or (in the normal colon and small intestine) desmin. They play a fundamental role in growth and development of intestine, immunology, inflammation, fibrosis, water and electrolyte transport, contraction and wound healing.

Cryopreserved CloneticsTM Human Intestinal Myofibroblasts (H-InMyoFib) are guaranteed through 15 population doublings, express alpha SM Actin after thaw ≥70% and Desmin ≤10%. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Recommended Medium: SMGMTM-2 BulletKitTM

Lonza 提供人类肠道肌成纤维细胞 (H-InMyoFib)，在第 2 代冷冻保存，每瓶 ≥500,000 个细胞。 H-InMyoFibs 具有成纤维细胞和平滑肌细胞的表型特征，但不表达平滑肌标志物，如平滑素、caldesmon 或（在正常结肠和小肠中）结蛋白。 它们在肠道的生长和发育、免疫学、炎症、纤维化、水和电解质运输、收缩和伤口愈合中发挥着重要作用。

冷冻保存的 CloneticsTM 人肠道肌成纤维细胞 (H-InMyoFib) 保证通过 15 次群体倍增，解冻后表达 α SM 肌动蛋白≥70% 和 Desmin ≤10%。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

推荐培养基：SMGMTM-2 BulletKitTM

Benefits

  • Absorption studies & toxicology pertaining to oral medications
  • Inflammation
  • Fibrosis
  • Water & electrolyte transport
  • Contraction & peristalsi
  • Wound healing
  • Neoplasia & intestinal polyp formation
  • Crohn’s disease
  • Toxicology and cytotoxicity
  • Gene expression

Content & Storage

Content

1 x Cryopreserved ampule InMyoFib containing ≥500,000 cells

Instructions, SDS, CoA

Related Products

InMyoFib – Human Intestinal Myofibroblasts
RPTEC – Human Renal Proximal Tubule Epithelial Cells CC-2553
RPTEC – 人肾近端小管上皮细胞 CC-2553
InMyoFib – Human Intestinal Myofibroblasts
InEpC – Human Intestinal Epithelial Cells CC-2931
InEpC – 人肠上皮细胞 CC-2931
InMyoFib – Human Intestinal Myofibroblasts
Human Intestinal Epithelial and Myofibroblast Cell Combo CC-4540
人肠上皮和肌成纤维细胞组合 CC-4540
InMyoFib – Human Intestinal Myofibroblasts
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞

lonza,lonza 2W-400C,lonza CC-2702,原代细胞和干细胞,人外周血 CD14+ 单核细胞

Catalog #: 2W-400C

Cryopreserved ampule of Human Peripheral Blood CD14+ Monocytes, positive selection, containing ≥10 million cells

人外周血 CD14+ 单核细胞冻存安瓿，阳性选择，含有≥1000 万个细胞

Product Overview

Monocytes are found in the circulating peripheral blood where they make up 10-20% of the total mononuclear cell population. Monocytes are characterized by their phenotypic expression of CD14, and thus, are commonly called CD14+ monocytes. They play an important role in host defense as circulating monocytes and differentiation into tissue macrophages, and can differentiate into dendritic cells with potent antigen-presenting capability.

单核细胞存在于循环外周血中，占总单核细胞群的 10-20%。 单核细胞的特征在于它们的 CD14 表型表达，因此，通常称为 CD14+ 单核细胞。 它们作为循环单核细胞和分化成组织巨噬细胞在宿主防御中发挥重要作用，并且可以分化成具有强大抗原呈递能力的树突状细胞。

Cells You Can Count on to Perform

Lonza Human Peripheral Blood CD14+ Monocytes are isolated from peripheral blood of screened, healthy, untreated (non-mobilized peripheral blood) donors. Briefly, mononuclear cells (MNCs) are isolated from apheresis derived peripheral blood. The MNC population is then further purified via positive immunomagnetic selection directed against CD14. Cryopreserved CD14+ Monocytes are guaranteed to express CD14 and will contain either ≥10, 20, or 40 million viable cells per ampoule depending upon the product ordered. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Lonza Human Peripheral Blood CD14+ 单核细胞是从经过筛选的、健康的、未经治疗的（未动员的外周血）供体的外周血中分离出来的。 简而言之，单核细胞 (MNC) 是从单采术衍生的外周血中分离出来的。 然后通过针对 CD14 的阳性免疫磁性选择进一步纯化 MNC 群体。 冷冻保存的 CD14+ 单核细胞保证表达 CD14，并且根据订购的产品，每个安瓿将包含 ≥10、20 或 4000 万个活细胞。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Choices in Cell Type and Tissue Source

Hematopoietic and immune research requires a variety of donors and cell types. That’s why Lonza offers many different peripheral blood cell types from a variety of donors, including PBMCs and dendritic cells, among others. In addition, if you are interested in exploring the differences between monocytes in different maturation states or from different tissues, we offer bone marrow mononuclear cells.

造血和免疫研究需要多种供体和细胞类型。 这就是为什么 Lonza 提供来自各种供体的多种不同外周血细胞类型，包括 PBMC 和树突状细胞等。 此外，如果您有兴趣探索不同成熟状态或来自不同组织的单核细胞之间的差异，我们提供骨髓单核细胞。

Supported by Lonza Media

The recommended medium varies by application (see below). Please contact Scientific Support for additional culturing recommendations. Please note, media listed for macrophage and dendritic cell differentiation are recommendations only.

  • Monocyte Phenotype: RPMI + 10% Fetal Bovine Serum + 2 mM L-glutamine + 10 ng/ml rHU M-CSF (optional)
  • Macrophages: RPMI +10% Fetal Bovine Serum + 2 mM L-glutamine + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids + 25 ng/ml rHU M-CSF
  • Dendritic Cells: LGM-3TM Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4

Lonza CD14+ monocytes are not routinely quality control tested for macrophage or dendritic cell differentiation and differentiation is not guaranteed under the cell warranty. Additional factors including extracellular matrixes or culturing conditions may be required to produce differentiation.

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

Lonza CD14+ 单核细胞没有针对巨噬细胞或树突状细胞分化进行常规质量控制测试，并且细胞保修不保证分化。 产生分化可能需要其他因素，包括细胞外基质或培养条件。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。 如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis (CoA) is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of CD14+ Monocytes containing ≥ 10 million cells

Instructions, SDS, CoA

Related Products
Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 10 Million
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702
人外周血单个核细胞 (hPBMC)，冷冻保存，5000 万个 CC-2702
Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 10 Million
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218
Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 10 Million

Data
Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 10 Million

CC-3208,lonza CC-3207,原代细胞和干细胞,OBM™ 成骨细胞生长和分化基础培养基

Catalog #: CC-3208
OBMTM Osteoblasts Basal Medium

Product Overview

OBMTM 是一种成骨细胞生长基础培养基，单独出售。 这可以与 1 套 CC-4193（成骨细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。 由基础培养基和补充剂组成的完整 OGMTM BulletKitTM 可以使用目录号 CC-3207 一起购买。

CloneticsTM OGMTM 成骨细胞生长培养基专为人类成骨细胞的生长和扩增而配制。 该试剂盒含有 10% 的血清。 OGMTM 分化补充剂也包括在内，建议用于分化测定。 该试剂盒包装方便，包括含有生长和分化补充剂的基础培养基，用于成骨细胞的完全培养

Benefits

Applications:

  • In vitro bone models
  • Mineralization
  • Osteoporosis
  • Gene expression
  • Hormone treatments
  • Osteopenia
  • Paget’s Disease

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Related Products
OBM™ Osteoblast Growth and Differentiation Basal Medium
NHOst – Human Osteoblasts CC-2538

NHOst – 人类成骨细胞 CC-2538

OBM™ Osteoblast Growth and Differentiation Basal Medium
OGM™ Osteoblast Growth Medium BulletKit™ CC-3207
OGM™ 成骨细胞生长培养基 BulletKit™ CC-3207

lonza PT-4124,原代细胞和干细胞,用于 hMSC 软骨分化试剂盒的 TGF Beta 3

Catalog #: PT-4124

软骨分化所需的 TGF Beta-3，2 µg。 用于 hMSC 软骨分化 BulletKitTM，PT-3003（单独出售）

Product Overview

TGF Beta 3 required for use with the hMSC Chondrogenic Differentiation BulletKitTM (PT-3003).

Content & Storage

Content

1 x Lyophilized TGF Beta 3, 2 µg

Related Products

TGF Beta 3 for hMSC Chondrogenic Differentiation Kit
NHAC-kn – Human Knee Articular Chondrocytes CC-2550

NHAC-kn – 人膝关节软骨细胞 CC-2550

TGF Beta 3 for hMSC Chondrogenic Differentiation Kit
CGM™ Chondrocyte Growth Medium BulletKit™ CC-3216

CGM™ 软骨细胞生长培养基 BulletKit™ CC-3216

TGF Beta 3 for hMSC Chondrogenic Differentiation Kit
CDM™ Chondrocyte Differentiation Medium BulletKit™ CC-3225

CDM™ 软骨细胞分化培养基 BulletKit™ CC-3225

TGF Beta 3 for hMSC Chondrogenic Differentiation Kit
Chondrocyte ReagentPack™ Subculture Reagents CC-3233

软骨细胞 ReagentPack™ 继代培养试剂 CC-3233

TGF Beta 3 for hMSC Chondrogenic Differentiation Kit
DPSC – Human Dental Pulp Stem Cells PT-5025
TGF Beta 3 for hMSC Chondrogenic Differentiation Kit
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
 MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza M-FB-481,原代细胞和干细胞,小鼠胚胎成纤维细胞 (MEF)

Catalog #: M-FB-481
Cryopreserved ampule of Mouse Embryonic Fibroblasts containing ≥2 million cells

Product Overview

冷冻保存的小鼠原代胚胎成纤维细胞 (MEF) 从 CD-1 小鼠胚胎中分离出来，通过培养扩增，然后冷冻保存。

细胞可保证 5 次群体倍增，并显示出与新鲜细胞相当的形态和生长特性。 CloneticsTM MEF 方便易用，使研究人员能够专注于结果。

推荐培养基：Lonza’s DMEM High Glucose w/ L-Gln，500 mL（第 12-604F 部分），含 10% FBS（Sigma-Aldrich 第 F6178 部分）

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Content & Storage

Content

≥ 2 million cryopreserved cells in a 1.0 mL cell suspension

Related Products

Mouse Embryonic Fibroblasts (MEF)
P4 Primary Cell 4D-Nucleofector™ X Kit L V4XP-4012

P4 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-4012

Mouse Embryonic Fibroblasts (MEF)
P4 Primary Cell 4D-Nucleofector™ X Kit L V4XP-4024

P4 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-4024

Mouse Embryonic Fibroblasts (MEF)
P4 Primary Cell 4D-Nucleofector™ X Kit S V4XP-4032

P4 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-4032

Mouse Embryonic Fibroblasts (MEF)
P4 Primary Cell 96-well Nucleofector™ Kit V4SP-4096
Mouse Embryonic Fibroblasts (MEF)
P4 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-4960
Mouse Embryonic Fibroblasts (MEF)
P4 Primary Cell 384-well Nucleofector™ Kit V5SP-4002

P4 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-4002

Mouse Embryonic Fibroblasts (MEF)
P4 Primary Cell 384-well Nucleofector™ Kit V5SP-4010
P4 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-4010

lonza CC-4009,原代细胞和干细胞,牛垂体提取物 (BPE)

Catalog #: CC-4009
Bovine Pituitary Extract (BPE), 13mg/mL, 2 mL

Product Overview

牛垂体提取物可用于培养各种上皮细胞类型。 该产品包含在与用于上皮细胞类型的各种培养基系统相关的 SingleQuotsTM 试剂盒中。 该产品的浓度为 13 mg/ml。

Content & Storage

Content

1 x Vial Containing 13 mg/mL BPE 2 mL

lonza CC-3246,原代细胞和干细胞,SKBM™- 2 骨骼肌细胞生长基础培养基-2

Catalog #: CC-3246

SkBMTM-2 Skeletal Muscle Myoblasts Basal Medium for culture of human skleletal muscle mycoblasts, 500 mL

Product Overview

SkBMTM -2 是一种骨骼肌成肌细胞基础培养基，单独出售。 这可以与 1 套 CC-3244（骨骼肌成肌细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。 由基础培养基和补充剂组成的完整 SkGMTM -2 BulletKitTM 可以使用目录号 CC-3245 一起购买。 我们提供两种骨骼肌生长培养基 – SkGMTM 和 SkGMTM-2。

SkGMTM-2 骨骼肌细胞生长培养基-2（目录号 CC-3245）是 SkGMTM 培养基的增强版。 胎球蛋白已被去除，基础培养基也得到了进一步优化。

SkGMTM 培养基经过优化，可用于人体骨骼肌细胞。 SkGMTM -2 专为与正常和糖尿病骨骼肌成肌细胞一起使用而设计

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Related Products

SKBM™- 2 Skeletal Muscle Cell Growth Basal Medium-2
HSMM – Human Skeletal Muscle Myoblasts CC-2580

HSMM – 人体骨骼肌成肌细胞 CC-2580

SKBM™- 2 Skeletal Muscle Cell Growth Basal Medium-2
D-HSMM – Diseased Human Skeletal Muscle Myoblasts – Diabetes Type I CC-2900

D-HSMM – 患病的人体骨骼肌成肌细胞 – I 型糖尿病 CC-2900

SKBM™- 2 Skeletal Muscle Cell Growth Basal Medium-2
D-HSMM – Diseased Human Skeletal Muscle Myoblasts – Diabetes Type II CC-2901

D-HSMM – 患病的人体骨骼肌成肌细胞 – II 型糖尿病 CC-2901

SKBM™- 2 Skeletal Muscle Cell Growth Basal Medium-2
SkGM™-2 Skeletal Muscle Cell Growth Medium-2 BulletKit™ CC-3245
SkGM™-2 骨骼肌细胞生长培养基-2 BulletKit™ CC-3245

lonza,lonza CC-2551B,原代细胞和干细胞,乳腺上皮细胞培养试剂盒

货号: CC-2551B
Mammary Epithelial Cell Culture Kit
乳腺上皮细胞培养试剂盒

Product Overview

Purchase our Mammary Epithelial Cell Culture Kit (catalog no. CC-2551B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of mammary epithelial cells (catalog no. CC-2551) and MEGMTMTM BulletKitTM Mammary Epithelial Cell Growth Media (catalog no. CC-3150)

购买我们的乳腺上皮细胞培养试剂盒（目录号 CC-2551B）并获得细胞和培养基的折扣价。 该试剂盒由冷冻保存的乳腺上皮细胞安瓿（货号 CC-2551）和 MEGMTMTM BulletKitTM 乳腺上皮细胞生长培养基（货号 CC-3150）组成

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial
1 x Bottle Basal Medium 500 mL
1 x Orange Cap Vial with BPE 2.00 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – German
SDS – Italian
SDS – Dutch
SDS – French
SDS – Spanish
SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

lonza CC-3205,lonza CC-4181,CC-3204,SCGM™ 基质细胞生长培养基 BulletKit™

货号: CC-3205
SCGMTM Stromal Cell BulletKitTM
SCGMTM 基质细胞 BulletKitTM

Product Overview

Stromal Cell Growth Medium was developed to support the maintenance and expansion of prostate stromal cells and certain aortic and dental fibroblasts. SCGMTMmedium contains 5% FBS.

CloneticsTM Media Products are specially designed to support the growth of human and animal primary derived cells. Media systems have been specifically developed and optimized for various cell systems.

基质细胞生长培养基用于支持前列腺基质细胞和某些主动脉和牙成纤维细胞的维持和扩增。 SCGMTMmedium 含有 5% FBS。

CloneticsTM 培养基产品专为支持人和动物原代细胞的生长而设计。 媒体系统已针对各种细胞系统专门开发和优化。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Gray Cap Vial with hFGF-B 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Bottle FBS 25.00 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – German
SDS – Italian
SDS – Dutch
SDS – French
SDS – Spanish
SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

 

Related Products

SCGM™ Stromal Cell Growth Medium BulletKit™
AoAF – Human Aortic Adventitial Fibroblasts CC-7014

AoAF – 人主动脉外膜成纤维细胞 CC-7014

SCGM™ Stromal Cell Growth Medium BulletKit™
PrSC – Human Prostate Stromal Cells CC-2508

PrSC – 人类前列腺基质细胞 CC-2508

SCGM™ Stromal Cell Growth Medium BulletKit™
HPdLF – Human Periodontal Ligament Fibroblasts CC-7049

HPdLF – 人牙周膜成纤维细胞 CC-7049

SCGM™ Stromal Cell Growth Medium BulletKit™
SCBM™ Stromal Cell Growth Basal Medium CC-3204

SCBM™ 基质细胞生长基础培养基 CC-3204

SCGM™ Stromal Cell Growth Medium BulletKit™
SCGM™ Stromal Cell Growth Medium SingleQuots™ Supplements and Growth Factors CC-4181
SCGM™ 基质细胞生长培养基 SingleQuots™ 添加剂和生长因子 CC-4181

lonza,lonza 2M-125E,原代细胞和干细胞,人骨髓单个核细胞 (MNC)

货号: 2M-125E

Cryopreserved human bone marrow mononuclear cells, 300 million cells

冷冻保存的人骨髓单个核细胞，3亿个细胞

Product Overview

Bone marrow mononuclear cells are a mixed population of single nucleus cells including monocytes, lymphocytes, and hematopoietic stem and progenitor cells.

骨髓单核细胞是单核细胞的混合群体，包括单核细胞、淋巴细胞以及造血干细胞和祖细胞。

BMNCs You Can Count on to Perform

Cryopreserved Lonza Bone Marrow Mononuclear Cells are isolated from whole bone marrow via density gradient separation. Isolating mononuclear cells eliminates erythrocytes and graulocytes, producing a more stable cell product. Lonza BMNCs are available  from a large and varied donor pool. They are guaranteed to contain either ≥5, 25, 100, or 200 million viable cells per ampoule, depending upon product purchased.

Fresh BMNC orders require a minimum order quantity of two vials per donor. Please contact Customer Service for information on sizes and pricing for fresh cells.

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support. Minimum order quantity of 2 vials per donor is required for fresh cells. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.’

通过密度梯度分离从全骨髓中分离冷冻保存的龙沙骨髓单核细胞。分离单核细胞消除红细胞和粒细胞，产生更稳定的细胞产物。 Lonza BMNCs 可从大量多样的供体库中获得。根据购买的产品，它们保证每个安瓿含有 ≥5、25、100 或 2 亿个活细胞。

新鲜的 BMNC 订单要求每个捐赠者至少订购两瓶。有关新鲜细胞的尺寸和价格信息，请联系客户服务。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。对于新鲜细胞，每个供体最少需要订购 2 瓶。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Content & Storage

Content

1 cryopreserved ampule containing ≥ 300 million cells

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

lonza CC-4068,原代细胞和干细胞,hFGF-人成纤维细胞生长因子CC-4068

Catalog #: CC-4068
hFGF, Human Fibroblast Growth Factors, 1 mL

Product Overview

成纤维细胞生长因子 (FGFs) 是参与血管生成、伤口愈合、胚胎发育和各种内分泌信号通路的生长因子的组合。 FGF 是肝素结合蛋白，与细胞表面相关的硫酸乙酰肝素蛋白聚糖的相互作用已被证明对 FGF 信号转导至关重要。 FGF 是多种细胞和组织增殖和分化过程中的关键参与者。 重组纤维母细胞生长因子的浓度为 1µg/ml

Content & Storage

Content

1 x Vial Containing 1 ug/mL hFGFTM, 1 mL

lonza PT-5001,原代细胞和干细胞,HprAD-人皮下前脂肪细胞

Catalog #: PT-5001

Cryopreserved ampule of Human Subcutaneous Preadipocytes containing ≥4 million cells

Product Overview

PoieticsTM 正常人前脂肪细胞 (HprAD) 是从正常（非糖尿病）供体的皮下或内脏脂肪中分离出来的。 来自糖尿病供体的前脂肪细胞可作为单独的产品获得。

相关的额外捐赠者信息，包括体重指数 (BMI) 可通过联系科学支持获得。

冷冻保存的 PoieticsTM 正常人前脂肪细胞 (HprAD) 保证每安瓿含有≥100 万或≥400 万（仅皮下）细胞，并且脂肪分化检测呈阳性。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Recommended Media and Reagents 

  • PGMTM -2 Preadipocyte Growth Medium-2 BulletKitTM Medium (provides both a proliferation medium and a differentiation medium guaranteed to induce differentiation into mature, functionally active adipocytes; Each bottle of the PGM-2TM BulletKitTM Medium contains enough differentiation medium to fully differentiate 10 million preadipocytes [10 complete 96-well plates] into mature, functionally active adipocytes).

PGMTM-2 培养基以 BulletKitTM 培养基（目录号 PT-8002）的形式提供，其中包括基础培养基和前脂肪细胞增殖和分化所需的补充剂。 基础培养基（PGMTM-2 基础培养基；目录号 PT-8202）和必要的补充剂（PGMTM-2 SingleQuotTM 试剂盒；目录号 PT-9502）也可以单独购买。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of Subcutaneous Preadipocytes containing ≥ 4 million cells

Related Products

HprAD - Human Subcutaneous Preadipocyte Cells
PBM-2 Preadipocyte Basal Medium-2 PT-8202

PBM-2 前脂肪细胞基础培养基-2 PT-8202

HprAD - Human Subcutaneous Preadipocyte Cells
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1012

HprAD - Human Subcutaneous Preadipocyte Cells
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024
HprAD - Human Subcutaneous Preadipocyte Cells
P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032

P1 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-1032

HprAD - Human Subcutaneous Preadipocyte Cells
P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-1096

HprAD - Human Subcutaneous Preadipocyte Cells
P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-1960

HprAD - Human Subcutaneous Preadipocyte Cells
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002
HprAD - Human Subcutaneous Preadipocyte Cells
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010

P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1010

HprAD - Human Subcutaneous Preadipocyte Cells
Human Visceral Preadipocyte Cells PT-5005

人内脏前脂肪细胞 PT-5005

HprAD - Human Subcutaneous Preadipocyte Cells
Human Subcutaneous Preadipocyte Cells PT-5020

人皮下前脂肪细胞 PT-5020

HprAD - Human Subcutaneous Preadipocyte Cells
AdipoRed™ Assay Reagent, 20 mL PT-7009
HprAD - Human Subcutaneous Preadipocyte Cells
hADSC Human Adipose-Derived Stem Cells PT-5006

hADSC 人体脂肪干细胞 PT-5006

HprAD - Human Subcutaneous Preadipocyte Cells
hMSC – Human Mesenchymal Stem Cells PT-2501

hMSC – 人间充质干细胞 PT-2501

HprAD - Human Subcutaneous Preadipocyte Cells
Diseased Human Visceral Preadipocyte Cells – Diabetes Type I PT-5023

患病的人类内脏前脂肪细胞 – I 型糖尿病 PT-5023

HprAD - Human Subcutaneous Preadipocyte Cells
Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type I PT-5021

患病的人类皮下前脂肪细胞 – I 型糖尿病 PT-5021

HprAD - Human Subcutaneous Preadipocyte Cells
Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II PT-5022
患病的人类皮下前脂肪细胞 – II 型糖尿病 PT-5022

lonza R-EDRG-515,原代细胞和干细胞,大鼠胚胎背根神经节(eDRG)神经元

Catalog #: R-EDRG-515
Cryopreserved Rat Embryonic Dorsal Root Ganglion ampule containing ≥ 1,000,000 cells

Product Overview

即用型大鼠胚胎背根神经节 (DRG) 神经元是通过标准化方法制备的高质量感觉神经元的悬浮液，可立即进行培养。

每瓶胚胎背根神经节神经元在 0.25 ml 悬浮液中含有大约 100 万个细胞。在电镀后的最初几天会发生一些细胞死亡，并且会观察到碎片。这很正常。培养大约 4 天后，细胞将形成神经突网络，到第 7 天，碎片将最少。在没有有丝分裂抑制剂的情况下，神经元倾向于聚集（神经节化）并从基质上分离。

大鼠胚胎背根神经节神经元 Tuj 染色阳性。原代神经元细胞需要合适的基质来粘附和存活。优选的底物是具有层粘连蛋白的聚-D-赖氨酸。聚-D-赖氨酸也可单独用于包被细胞培养塑料器皿或盖玻片。包被的细胞培养板、培养皿或盖玻片可以从供应商处购买，也可以在使用前立即制备。

推荐培养基：PNGMTM BulletKitTM 添加 100 ng/ml 大鼠神经生长因子-β (Sigma No. N2513)*

*重要提示：为了胚胎 DRG 神经元的存活和生长，始终需要向培养基中添加 100 ng/ml 的神经生长因子（Sigma No. N2513）。 为了抑制雪旺氏细胞增殖，还需要添加有丝分裂抑制剂。

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

Content & Storage

Content

≥ 1,000,000 cryopreserved cells per ampule

Related Products
Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons
PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461

PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024

P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024

Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096
Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza 4W-350,原代细胞和干细胞,人外周血 CD3+ Pan T 细胞

Catalog #: 4W-350
Human CD3+ T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 25 million cells, cryopreserved

Product Overview

Leukapheresed Pan T 细胞 (CD3+) 是使用间接免疫磁性标记系统从单核细胞中阴性选择的。 细胞在冷冻管中的 Lonza Cryo 培养基中冷冻保存。 解冻后使用 FACS 测定确定细胞纯度。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞。

由 AllCells® 制造

Applications

– CAR-T research

– Cell Therapy process development

– Immunology

– Immuno-oncology

Content & Storage

Content

1 vial containing ≥ 25 million cryopreserved cells

Related Products
Human Peripheral Blood CD3+ Pan T Cells, Cryopreserved, 25 Million
Human CD4+/CD45RA+ Naive T Cells 4W-202

人 CD4+/CD45RA+ 初始 T 细胞 4W-202

Human Peripheral Blood CD3+ Pan T Cells, Cryopreserved, 25 Million
Human CD4+/CD45RO+ Memory T Cells 4W-204

人 CD4+/CD45RO+ 记忆 T 细胞 4W-204

Human Peripheral Blood CD3+ Pan T Cells, Cryopreserved, 25 Million
Human Naïve CD8+/CD45RA+ T Cells 4W-302
人类幼稚 CD8+/CD45RA+ T 细胞 4W-302

lonza,原代细胞和干细胞,lonza L7™ hPSC Matrix

Catalog #: FP-5020

Recombinant, xeno free and defined L7TM hPSC Matrix, 1 mg for up to 1000 cm2

Product Overview

重组、无异源和明确的 hPSC 基质，以支持许多 hPSC 系的高效附着、保持多能性和稳定的核型。

Benefits

  • Recombinant, xeno-free and defined hPSC Matrix
  • Supports efficient hPSC attachment
  • Maintains pluripotency and stable karyotype

Content & Storage

Content

1 x L7TM hPSC Matrix, 1 mg for up to 1000 cm2

 

Related Products
L7™ hPSC Matrix
L7™ hPSC Passaging Solution FP-5013
L7™ hPSC 传代解决方案 FP-5013

lonza CC-2504,原代细胞和干细胞,NHEM-Neo-人类表皮黑色素细胞新生儿

Catalog #: CC-2504
NHEM-Neo, Epidermal Melanocytes, Neonatal

Product Overview

成人和新生儿人类表皮黑色素细胞 (NHEM) 是位于所有哺乳动物皮肤表皮层的色素生成细胞。黑色素细胞的功能是产生黑色素，黑色素是赋予皮肤颜色的色素。黑色素保护皮肤免受紫外线伤害。皮肤由2个主要层组成，表皮和真皮。表皮由多个角质形成细胞层组成。黑素细胞位于表皮的基底层。星状或树突状黑素细胞形态允许黑素细胞细胞体驻留在表皮的基底层，而树突与表皮上层的角质形成细胞结合。这种关联允许黑色素细胞将黑色素从细胞体通过树突运输到角质形成细胞中，从而导致皮肤着色。

黑素细胞研究有两个主要领域——化妆品和医学。化妆品研究中心专注于改变皮肤颜色的能力。医学研究通常集中在黑色素瘤以及各种皮肤病或相关疾病，如白化病和白癜风、激素治疗、甲状腺疾病、牛皮癣、烧伤和疤痕。

我们冷冻保存的正常人表皮黑色素细胞通过 8 次群体倍增保证，表达 Mel-5 和 L-Dopa 转化为黑色素。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

注意：黑色素细胞对过度胰蛋白酶化极为敏感。使用 Lonza 的胰蛋白酶/EDTA 溶液 (CC-5012) 并在使用前用 Versene®(17-711E) 进行 1:1 稀释。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products
NHEM-Neo – Human Epidermal Melanocytes, Neonatal
NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled 00192906

NHEK-Neo – 人表皮角质形成细胞，新生儿，汇集 00192906

NHEM-Neo – Human Epidermal Melanocytes, Neonatal
NHDF-Neo – Human Dermal Fibroblasts, Neonatal CC-2509
NHEM-Neo – Human Epidermal Melanocytes, Neonatal
NHEK-Neo – Human Epidermal Keratinocytes – Neonatal, Single Donor 00192907

NHEK-Neo – 人类表皮角质形成细胞 – 新生儿，单一供体 00192907

NHEM-Neo – Human Epidermal Melanocytes, Neonatal
HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Single Donor CC-2505

HMVEC-dNeo – 人真皮微血管内皮细胞，新生儿，单供体 CC-2505

NHEM-Neo – Human Epidermal Melanocytes, Neonatal
HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Pooled CC-2516
HMVEC-dNeo – 人真皮微血管内皮细胞，新生儿，混合 CC-2516

lonza CC-4092,原代细胞和干细胞,牛脑提取物 (BBE)

Catalog #: CC-4092
Bovine Brain Extract (3 mg/ml, 2 ml) contains endothelial cell proliferation growth factors

Product Overview

牛脑提取物的浓度为 3 mg/ml，单独出售或作为与内皮细胞培养基相关的 Lonza 的 SingleQuotsTM 试剂盒中的一个成分出售。

Content & Storage

Content

1 x Bottle containing 3 mg/mL BBE, 2 mL

Related Products
Bovine Brain Extract (BBE), 2 ml
EBM™ Endothelial Cell Growth Basal Medium, 500 mL CC-3121

EBM™ 内皮细胞生长基础培养基，500 mL CC-3121

Bovine Brain Extract (BBE), 2 ml
Bovine Brain Extract (BBE), 2 ml CC-4092
 牛脑提取物 (BBE)，2 ml CC-4092

lonza 2W-400B,原代细胞和干细胞,人外周血 CD14+ 单核细胞

Catalog #: 2W-400B
人外周血 CD14+ 单核细胞冻存安瓿，阳性选择，含有≥2000 万个细胞

Product Overview

单核细胞存在于循环外周血中，占总单核细胞群的 10-20%。单核细胞的特征在于它们的 CD14 表型表达，因此，通常称为 CD14+ 单核细胞。它们作为循环单核细胞和分化成组织巨噬细胞在宿主防御中发挥重要作用，并且可以分化成具有强大抗原呈递能力的树突状细胞。

您可以信赖的细胞

Lonza Human Peripheral Blood CD14+ 单核细胞是从经过筛选的、健康的、未经治疗的（未动员的外周血）供体的外周血中分离出来的。简而言之，单核细胞 (MNC) 是从单采术衍生的外周血中分离出来的。然后通过针对 CD14 的阳性免疫磁性选择进一步纯化 MNC 群体。冷冻保存的 CD14+ 单核细胞保证表达 CD14，并且根据订购的产品，每个安瓿将包含 ≥10、20 或 4000 万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

细胞类型和组织来源的选择

造血和免疫研究需要多种供体和细胞类型。这就是为什么 Lonza 提供来自各种供体的多种不同外周血细胞类型，包括 PBMC 和树突状细胞等。此外，如果您有兴趣探索不同成熟状态或来自不同组织的单核细胞之间的差异，我们提供骨髓单核细胞。

Supported by Lonza Media

The recommended medium varies by application (see below). Please contact Scientific Support for additional culturing recommendations. Media recommendations for macrophage and dendritic cell differentiation are recommendations only.

  • Monocyte Phenotype: RPMI + 10% Fetal Bovine Serum + 2 mM L-glutamine + 10 ng/ml rHU M-CSF (optional)
  • Macrophages: RPMI +10% Fetal Bovine Serum + 2 mM L-glutamine + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids + 25 ng/ml rHU M-CSF
  • Dendritic Cells: LGM-3TM Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4

Lonza CD14+ 单核细胞没有针对巨噬细胞或树突状细胞分化进行常规质量控制测试，并且细胞保修不保证分化。 产生分化可能需要其他因素，包括细胞外基质或培养条件。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of CD14+ Monocytes containing ≥ 20 million cells

Related Products
Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 20 million
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702

人外周血单个核细胞 (hPBMC)，冷冻保存，5000 万个 CC-2702

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 20 million
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 20 million

 Human CD14+ Monocytes

Human CD14+ monocytes differentiating to macrophages

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 20 million

lonza CC-3225,原代细胞和干细胞,CDM™ 软骨细胞分化培养基 BulletKit™

Catalog #: CC-3225
CDMTM Chondrocyte Differentiation BulletKitTM

Product Overview

  • CDMTM Chondrocyte Differentiation Medium is specially designed for the differentiation of chondrocytes. The BulletKitTM is conveniently packaged with basal medium and supplements included for the differentiation of chondrocytes.
  • CGMTM Chondrocyte Growth Medium is also available and sold separately for expansion of human chondrocytes

Content & Storage

Content

1 x Bottle Basal Medium 250 mL
1 x Yellow Cap Vial with R3-IGF 0.50 mL
1 x Blue Cap Vial with TGF-beta 1.25 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Red Cap Vial with GA-1000 0.25 mL
1 Bottle FBS 12.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL

Related Products
CDM™ Chondrocyte Differentiation Medium BulletKit™
NHAC-kn – Human Knee Articular Chondrocytes CC-2550
CDM™ Chondrocyte Differentiation Medium BulletKit™
CGM™ Chondrocyte Growth Medium BulletKit™ CC-3216

CGM™ 软骨细胞生长培养基 BulletKit™ CC-3216

CDM™ Chondrocyte Differentiation Medium BulletKit™
CDM™ Chondrocyte Differentiation Basal Medium CC-3226

CDM™ 软骨细胞分化基础培养基 CC-3226

CDM™ Chondrocyte Differentiation Medium BulletKit™
CDM™ Chondrocyte Differentiation Medium SingleQuots™ Supplements and Growth Factors CC-4408
CDM™ 软骨细胞分化培养基 SingleQuots™ 补充剂和生长因子 CC-4408

lonza CC-2579,原代细胞和干细胞,USMC-人脐动脉平滑肌细胞

Catalog #: CC-2579
UASMC, Umbilical Artery Smooth Muscle Cells

Product Overview

人血管平滑肌细胞来自多种组织类型，包括主动脉 (AoSMC)、冠状动脉 (CASMC)、肺动脉 (PASMC) 和脐动脉 (UASMC)。 冷冻保存的细胞在第 3 代运输。

CloneticsTM 人主动脉平滑肌细胞保证 15 次群体倍增，分化后 α 平滑肌肌动蛋白染色呈阳性，von Willebrand 因子 VIII 染色呈阴性。 所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。

Applications

  • Stroke
  • Diabetes
  • Vascular research
  • Artherosclerosis
  • Hypertension

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products
UASMC – Human Umbilical Artery Smooth Muscle Cells
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2 C2517A

HUVEC – EGM™-2 C2517A 中的人脐静脉内皮细胞，单一供体

UASMC – Human Umbilical Artery Smooth Muscle Cells
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™ CC-2517

HUVEC – EGM™ CC-2517 中的人脐静脉内皮细胞，单一供体

UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1012

UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024
UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032

P1 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-1032

UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-1096

UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-1960

UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002
UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010

P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1010

lonza PT-8202,原代细胞和干细胞,PBM-2 前脂肪细胞基础培养基-2

Catalog #: PT-8202
PGMTM-2 Basal Medium, 500 mL

Product Overview

PGMTM -2 前脂肪细胞生长培养基-2 BulletKitTM 培养基提供增殖培养基和分化培养基，保证诱导分化成成熟的、功能活跃的脂肪细胞； 每瓶 PGM-2TM BulletKitTM 培养基都含有足够的分化培养基，可将 1000 万个前脂肪细胞 [10 个完整的 96 孔板] 完全分化为成熟的功能活跃的脂肪细胞。 PGMTM-2 培养基以 BulletKitTM 培养基（目录号 PT-8002）的形式提供，其中包括基础培养基和前脂肪细胞增殖和分化所需的补充剂。 基础培养基（PGMTM-2 基础培养基；目录号 PT-8202）和必要的补充剂（PGMTM-2 SingleQuotTM 试剂盒；目录号 PT-9502）也可以单独购买。

lonza FP-5013,原代细胞和干细胞,lonza L7™ hPSC 传代解决方案

Catalog #: FP-5013
L7TM hPSC Passaging Solution, 100 mL

Product Overview

Non-enzymatic cell detachment formulation that gently dislodges multicellular colony fragments without the need for mechanical manipulation.  This user friendly method simplifies routine maintenance of your valuable hESC and iPSC lines.

无需机械操作即可温和去除多细胞集落碎片的非酶细胞分离配方。 这种用户友好的方法简化了您宝贵的 hESC 和 iPSC 生产线的日常维护。

lonza CC-4510,原代细胞和干细胞,内皮素 3 生长补充剂 (ET-3)

Catalog #: CC-4510
Endothelin-3 (ET-3), 130 µg

Product Overview

ET-3 生长补充剂是成人黑色素细胞生长所需的额外生长因子。 它单独出售以补充 MGMTM-4 BulletKitTM（目录号 CC-3249），以获得专门为生长成人黑色素细胞而设计的完整配方。 除了限制培养中的其他细胞类型污染外，ET-3 还提供增强的生长和铺板优势。

Content & Storage

Content

1 x Lyophilized Vial Containing 130 ug

Related Products
Endothelin-3 Growth Supplement (ET-3)
MGM™-4 Melanocyte Growth Medium-4 BulletKit™ CC-3249

MGM™-4 黑色素细胞生长培养基-4 BulletKit™ CC-3249

Endothelin-3 Growth Supplement (ET-3)
NHEM-Ad – Human Epidermal Melanocytes, Adult CC-2586
 NHEM-Ad – 人表皮黑色素细胞，成人 CC-2586

lonza M-ASM-330,原代细胞和干细胞,小鼠 C57 脑混合星形胶质细胞

Catalog #: M-ASM-330

Cryopreserved ampule of Mouse C57 Mixed Astrocytes containing ≥ 1 million cells

Product Overview

即用型 CloneticsTM 小鼠脑混合星形胶质细胞 (M-ASM-330) 从 C57 黑小鼠脑中获得，传代一次并制备成细胞悬浮液以供运输。

每个小瓶包含大约 100 万个细胞。 星形胶质细胞可以很容易地解冻和培养。 星形胶质细胞可以收获和重新铺板。 细胞在干冰上运输过夜。

推荐介质：AGMTM BulletKitTM (CC-3186)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

Content & Storage

Content

≥ 1,000,000 cryopreserved cells per ampule

Related Products
Mouse C57 Brain Mixed Astrocytes
Mouse CD1 Brain Mixed Astrocytes M-ASM-430

小鼠 CD1 脑混合星形胶质细胞 M-ASM-430

Mouse C57 Brain Mixed Astrocytes
AGM™ Astrocyte Growth Medium BulletKit™ CC-3186

AGM™ 星形胶质细胞生长培养基 BulletKit™ CC-3186

Mouse C57 Brain Mixed Astrocytes
Mouse Neuron Nucleofector™ Kit VPG-1001

小鼠神经元 Nucleofector™ 试剂盒 VPG-1001

Mouse C57 Brain Mixed Astrocytes
Mouse Neuron Nucleofector™ Kit VAPG-1001
Mouse C57 Brain Mixed Astrocytes
Mouse Neuron Nucleofector™ Kit VVPG-1001
Mouse C57 Brain Mixed Astrocytes
Rat Brain Cx-Hi-Cp Mixed Astrocytes R-ASM-530

大鼠脑 Cx-Hi-Cp 混合星形胶质细胞 R-ASM-530

Mouse C57 Brain Mixed Astrocytes
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
Mouse C57 Brain Mixed Astrocytes
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024

P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024

Mouse C57 Brain Mixed Astrocytes
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

Mouse C57 Brain Mixed Astrocytes
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096
Mouse C57 Brain Mixed Astrocytes
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

Mouse C57 Brain Mixed Astrocytes
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

Mouse C57 Brain Mixed Astrocytes
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza,lonza 2M-101,LT07-218,PT-2501,原代细胞和干细胞,人骨髓 CD34+ 祖细胞，100,000

Catalog #: 2M-101

Cryopreserved ampule of Human Bone Marrow CD34+ containing ≥100,000 cells. Mononuclear cells are isolated using positive immunomagnetic selection.

冷冻保存的人骨髓 CD34+ 安瓿，含有 ≥100,000 个细胞。 使用阳性免疫磁性选择分离单核细胞。

Product Overview

Bone marrow CD34+ cells contain hematopoietic stem and progenitor cells and are known to differentiate into all the various blood cell types.

Purity and Viability You Can Count On

Lonza bone marrow CD34+ cells are isolated from bone marrow mononuclear cells (MNC) by positive immunomagnetic separation and are guaranteed to have a purity of ≥90%. They are guaranteed to contain either ≥100,000, ≥300,000, ≥500,000, ≥1 million, or ≥2 million viable cells per ampoule depending on the product ordered. HIV-1, hepatitis C, and hepatitis B are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Fresh bone marrow CD34+ cells are offered in various packaging sizes and require a minimum order quantity of two vials per donor. Please contact Customer Service for information on sizes and pricing for fresh cells.

Lonza骨髓CD34+细胞通过阳性免疫磁分离从骨髓单个核细胞（MNC）中分离出来，保证纯度≥90%。 根据订购的产品，它们保证每个安瓿含有≥100,000、≥300,000、≥500,000、≥100 万或 ≥200 万个活细胞。 并非所有供体和/或细胞批次都检测到 HIV-1、丙型肝炎和乙型肝炎。 为购买的每个细胞批次提供分析证书。 新鲜骨髓 CD34+ 细胞有多种包装尺寸，每个供体至少需要订购两瓶。 有关新鲜细胞的尺寸和价格信息，请联系客户服务。

Supported by an Optimized Culture System

HPGMTM Hematopoietic Growth Medium is recommended for optimal performance in serum-free culturing (additional cytokines may be necessary depending on application) Alternative media containing serum may also be acceptable. Please contact Scientific Support for serum containing media recommendations.

建议使用 HPGMTM 造血生长培养基以在无血清培养中获得最佳性能（根据应用可能需要额外的细胞因子） 也可以接受含有血清的替代培养基。 有关含血清培养基的建议，请联系科学支持。

Choices in Cell Type and Tissue Source

Complex research often calls for flexibility. That’s why we offer isolated and purified bone marrow CD34+ cells as well as unprocessed bone marrow in case you need to isolate your own cells. Bone marrow CD34+ cells are ideal for studies requiring a large number of cells from a single donor or studies investigating differences in cells of donors of various ages, genders, or ethnicities. If however, your studies demand more naïve cells, cord blood CD34+ cells may be the better choice.

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

复杂的研究通常需要灵活性。 这就是为什么我们提供分离和纯化的骨髓 CD34+ 细胞以及未加工的骨髓，以防您需要分离自己的细胞。 骨髓 CD34+ 细胞非常适用于需要来自单个供体的大量细胞的研究或调查不同年龄、性别或种族供体的细胞差异的研究。 但是，如果您的研究需要更多幼稚细胞，脐带血 CD34+ 细胞可能是更好的选择。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。 如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。

Benefits

  • Ready to use
  • High purity with > 90% CD34+ cells
  • Wide donor variety
  • Large lot sizes (if large lot sizes are not required and more naive HSCs are desirable you may be interested in cord blood derived CD34+/- cells)

Content & Storage

Content

1 x Cryopreserved ampule Human Bone Marrow CD34+ containing ≥100,000 cells

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

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Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

 

 

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人骨髓基质细胞，500 万个 2M-302

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hMSC – Human Mesenchymal Stem Cells PT-2501

hMSC – 人间充质干细胞 PT-2501

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MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
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lonza 3W-270,原代细胞和干细胞,lonza 新鲜人外周血单个核细胞 (MNcs)

Catalog #: 3W-270
Mononuclear cells from human peripheral blood, fresh, 100 million cells

Product Overview

使用 Spectra Optia™ 单采机收集单核细胞 (MNC)。 白细胞被耗尽RBC并洗涤以去除血小板。 我们典型的 MNC 产品包含大约 50% 的 T 细胞、20 个单核细胞、10 个 B 细胞、10% 的 NK 细胞，并且可能含有高达 5% 的粒细胞。 按照 IRB 协议从健康供体的离心管中收集细胞。 由 AllCells® 制造 新鲜的人源产品需支付额外运费

Content & Storage

Content

1 vial containing ≥ 20 million fresh cells

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lonza HUCS20P,原代细胞和干细胞,lonza DonorPlexTM 混合供体人肝细胞

Catalog #: HUCS20P
具有多种 CYP450、UGT、SULT 和转运蛋白活性特征的混合供体人肝细胞

Product Overview

对于 DMPK 研究而言，一致且可靠的汇集供体人肝细胞来源非常重要。 通过我们新的专有算法，我们能够预测 DonorPlexTM 人肝细胞的产量、活力和功能表型，这反过来将支持您的 DMPK 中更一致的结果。 每批汇集的 20 位供体、混合性别的人类肝细胞的特征在于活产率，以及 CYP1A2、CYP2A6、CYP2B6、CYP2C9、CYP2C19、CYP2E1、CYP3A4、UGT、SULT、OAT1B1/3、OCT 和 NTCP 活性。

凭借我们大量的单供体人类肝细胞库存，并使用我们的专有算法来预测表型，我们还可以定制生产具有表型的混合供体肝细胞批次，以满足您的需求。

DonorPlex™ 肝细胞 – 悬浮合格肝细胞非常适合短期（<4 小时）体外药物清除试验。 悬浮合格的肝细胞不适用于需要在粘附的单层中培养肝细胞的应用。

Benefits

  • Targeted pooling method for predictable metabolism
  • Precharacterized for 9 CYPs and 4 drug transporters
  • High viability and yields
  • Lot sizes < 2000 are possible

Applications

  • Suspension metabolism studies

Content & Storage

Content

1 x Cryopreserved ampule of Pooled, Suspension-Qualified Hepatocytes containing >5 million viable cells

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lonza 2S-101D,原代细胞和干细胞,人骨髓,未加工的人骨髓10 mL 1M-105

Catalog #: 2S-101D

含有≥500万个细胞的人骨髓单个核细胞的冷冻保存安瓿

Product Overview

骨髓单核细胞 (BMNC) 是单核细胞的混合群体，包括单核细胞、淋巴细胞以及造血干细胞和祖细胞。

通过密度梯度分离从全骨髓中分离冷冻保存的龙沙骨髓单核细胞。分离单核细胞消除红细胞和粒细胞，产生更稳定的细胞产物。

Lonza BMNCs 可从大量多样的供体库中获得新鲜*或冷冻。根据购买的产品，它们保证每个安瓿含有≥5、25、100、200 和 3 亿个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

细胞类型和组织来源的选择

Lonza 提供细胞类型和组织来源的选择，因此您可以选择适合您的研究和预算需求的产品。根据您选择的最终细胞，您可以选择未加工的骨髓、骨髓 MNC、骨髓 CD34+ 细胞、骨髓基质细胞（用作饲养细胞）或骨髓间充质干细胞。此外，如果骨髓不是您首选的组织来源，我们还提供脐带血来源的细胞。

Recommended Media and Reagents

  • HPGMTM Hematopoietic Growth Medium is recommended for optimal performance in serum-free culturing (additional cytokines may be necessary depending on application). Alternative media containing serum may also be acceptable. Please contact Scientific Support for serum containing media recommendations.

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。 如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。

*新鲜细胞悬浮在含有 5 mM EDTA 和 0.5% BSA 的 HBSS 中，每瓶包装尺寸有多种细胞可供选择。 有关新鲜细胞的尺寸和价格信息，请联系客户服务。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule BMNCs containing ≥5 million cells

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lonza M-CX-300,原代细胞和干细胞,小鼠 C57 脑皮层神经元

Catalog #: M-CX-300
冷冻保存的小鼠 C57 皮层神经元安瓿，含有≥400 万个细胞

Product Overview

即用型 CloneticsTM 小鼠脑 C57 皮层神经元 (M-CX-300) 是从小鼠 C57 黑脑 (E14, 15) 中分离出来的。 这些以标准化方法制备的高质量原代胚胎脑神经元细胞（包括神经胶质细胞）的细胞悬液形式出售，并可立即培养。

每瓶皮层细胞含有大约 400 万个活细胞。 这将使用推荐的电镀密度和培养基播种到 24 孔板的 10 个或更多孔和 96 孔板的 50 个或更多孔中。 培养约 4 天后，细胞将形成神经突网络，到第 7 天，碎片将最少。

推荐介质：PNGMTM BulletKitTM (CC-4461)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

Content & Storage

Content

≥4,000,000 cryopreserved cells per ampule

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lonza 4Y-101C,原代细胞和干细胞,来自动员外周血的人 CD34+ 祖细胞

Catalog #: 4Y-101C
来自动员外周血的人 CD34+ 祖细胞的冷冻保存安瓿，使用阳性免疫磁性选择，包含 ≥ 100 万个细胞

Product Overview

使用免疫磁性 CD34 微珠标记系统积极选择动员的外周血 CD34+ 造血干细胞和祖细胞。 细胞在冷冻管中的 Lonza Cryo 培养基中冷冻保存。 解冻后使用 FACS 测定确定细胞纯度。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞

由 AllCells® 制造

Benefits

  • Obtain large quantities of CD34+ from a donor
  • Certificate of analysis (CoA) provided for each lot purchased
  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots

Applications

  • CAR-T therapy development
  • iPSC generation
  • Autoimmune disease
  • Immunology
  • Cancer research

Content & Storage

Content

1 x Cryopreserved ampule of Human CD34+ Progenitor Cells from Mobilized Peripheral Blood containing ≥ 1 million cells

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lonza CC-3165,原代细胞和干细胞,PrEBM™ 前列腺上皮细胞生长基础培养基

Catalog #: CC-3165
PrEBMTM Prostate Epithelial Basal Medium

Product Overview

PrEBMTM 是一种前列腺上皮细胞生长基础培养基，单独出售。 这可以与 1 套 CC-4177（前列腺上皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。 由基础培养基和补充剂组成的完整 PrEGMTM BulletKitTM 可以使用目录号 CC-3166 一起购买。

PrEGMTM BulletkitTM，前列腺上皮细胞生长培养基专为人前列腺上皮细胞的维持和扩增而配制。 PrEGMTM 培养基不含血清。

Content & Storage

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1 x Bottle Basal Medium 500 mL

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lonza R-CXAS-520,原代细胞和干细胞,大鼠脑皮质星形胶质细胞

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Cryopreserved ampule of Rat Brain Cortex Astrocytes containing ≥ 1 million cells

Product Overview

即用型 CloneticsTM 大鼠脑皮层星形胶质细胞从大鼠脑中获得，传代一次并制备成细胞悬液，以便在干冰上运输。

每个小瓶包含大约 100 万个细胞。 星形胶质细胞可以很容易地解冻和培养。 星形胶质细胞可以收获并重新铺板或冷冻储存以备后用。 细胞在一夜之间运送。

推荐介质：AGMTM BulletKitTM (CC-3186)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
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Content & Storage

Content

1 x Cryopreserved ampule of Rat Brain Cortex Astrocytes containing ≥ 1 million cells

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Rat Brain Cortex Astrocytes
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Rat Brain Cortex Astrocytes
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

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P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

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lonza PT-3102A,原代细胞和干细胞,hMSC 脂肪生成维持培养基

Catalog #: PT-3102A

用于间充质干细胞成脂分化的维持培养基

Product Overview

脂肪生成维持培养基是 hMSC 脂肪生成分化 BulletKitTM 的一个组成部分，可在此处单独购买。

干细胞具有分化成包括脂肪细胞在内的多种细胞谱系的独特能力。 Lonza 的 hMSC 成脂分化 BulletKitTM 培养基是一种含血清的培养基系统，旨在诱导人骨髓来源的间充质干细胞在短短 19 天内成脂分化成成熟的、功能活跃的脂肪细胞。 然后可以通过多种方法验证分化，包括使用 Lonza 的 AdipoRedTM 脂肪生成分析试剂，该试剂提供与脂肪生成分化量相关的定量值。

Partial List of Cells Differentiated with hMSC Adipogenic Differentiation BulletKitTM Medium*:

  • Human bone marrow derived stem cells
  • C57BL/6 mice bone marrow derived stem cells
  • human adipose derived stem cells
  • Human periodontal ligament derived stem cells
  • Human hamstring tendon derived stem cells

hMSC 成脂分化 BulletKitTM 培养基提供诱导培养基和维持培养基，保证诱导人骨髓间充质干细胞成脂分化成成熟的、功能活跃的脂肪细胞；每瓶 hMSC 脂肪分化 BulletKitTM 培养基都含有足够的诱导培养基和维持培养基，可将大约 300 万个人骨髓来源的间充质干细胞 [6 孔板中大约 15 个孔] 完全分化为成熟的功能活跃的脂肪细胞。 hMSC 脂肪分化培养基以 BulletKitTM 培养基（目录号 PT-3004）的形式提供，其中包括用于诱导和维持的基础培养基以及用于脂肪形成诱导和维持人骨髓间充质干细胞的必要补充剂。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。

*从客户数据生成的单元格列表。 Lonza 仅常规地将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用，因此只能保证这种培养基与这些细胞一起成功使用。

lonza 2M-101B,原代细胞和干细胞,人骨髓 CD34+ 祖细胞

Catalog #: 2M-101B

来自骨髓的人 CD34+ 祖细胞的冷冻保存安瓿，含有 ≥ 500,000 个细胞。 使用阳性免疫磁性选择从单核细胞中分离细胞。

Product Overview

Bone marrow CD34+ cells contain hematopoietic stem and progenitor cells and are known to differentiate into all the various blood cell types.

Purity and Viability You Can Count On

Lonza骨髓CD34+细胞通过阳性免疫磁分离从骨髓单个核细胞（MNC）中分离出来，保证纯度≥90%。根据订购的产品，它们保证每个安瓿含有≥100,000、≥300,000、≥500,000、≥100 万或 ≥200 万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、丙型肝炎和乙型肝炎。为购买的每个细胞批次提供分析证书。提供各种大小的新鲜骨髓 CD34+ 细胞，每个供体至少需要两瓶。有关新鲜细胞的尺寸和价格信息，请联系客户服务。

建议使用 HPGMTM 造血生长培养基以在无血清培养中获得最佳性能（根据应用可能需要额外的细胞因子） 也可以接受含有血清的替代培养基。有关含血清培养基的建议，请联系科学支持。

复杂的研究通常需要灵活性。这就是为什么我们提供分离和纯化的骨髓 CD34+ 细胞以及未加工的骨髓，以防您需要分离自己的细胞。骨髓 CD34+ 细胞非常适用于需要来自单个供体的大量细胞的研究或调查不同年龄、性别或种族供体的细胞差异的研究。但是，如果您的研究需要更多幼稚细胞，脐带血 CD34+ 细胞可能是更好的选择。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Benefits

  • Ready to use
  • Wide donor variety
  • Large lot sizes (if large lot sizes are not required and more naive HSCs are desirable you may be interested in cord blood derived CD34+ cells)

Content & Storage

Content

1 x Cryopreserved ampule of Human Bone Marrow CD34+ containing ≥ 500,000 cells

Related Products
Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000
IMDM with L-Glutamine and HEPES 12-722F

含有 L-谷氨酰胺和 HEPES 12-722F 的 IMDM

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000
Human Bone Marrow Stromal Cells, 5 Million 2M-302

人骨髓基质细胞，500 万个 2M-302

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000
Unprocessed Human Bone Marrow, Fresh, 10 mL 1M-105
Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000
hMSC – Human Mesenchymal Stem Cells PT-2501

hMSC – 人间充质干细胞 PT-2501

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza,lonzaPT-3924,原代细胞和干细胞,hMSC – 人间充质干细胞成骨分化基础培养基

Catalog #: PT-3924
hMSC Osteogenic Differentiation Basal Medium, 170 mL
Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Certificate of Analysis (CoA)

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lonza00195130,原代细胞和干细胞,KBM™ Gold 角质形成细胞生长基础培养基，不含钙和酚红

Catalog #: 00195130
KBMTM Gold Keratinocyte Basal Medium, Calcium-free
KBMTM Gold 角质形成细胞基础培养基，无钙

Product Overview

KBMTM Gold is a calcium-free keratinocyte basal medium sold separately. This can be used with 1 kit of 192152 (keratinocyte cell growth medium SingleQuotsTM Supplements and Growth Factors). The full KGMTM Gold Calcium Free BulletKitTM consisting of basal medium and supplements can be purchased together using the catalog number 195769.

KGMTM Gold BulleKitTM is a new robust and versatile serum-free medium formulation for high and low density NHEK-Normal Human Epidermal Keratinocyte cultures

KBMTM Gold 是一种不含钙的角质形成细胞基础培养基，单独出售。 这可以与 1 套 192152（角质形成细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。 由基础培养基和补充剂组成的完整 KGMTM Gold Calcium Free BulletKitTM 可以使用目录号 195769 一起购买。

KGMTM Gold BulleKitTM 是一种新的稳健且多功能的无血清培养基配方，适用于高密度和低密度 NHEK-正常人表皮角质形成细胞培养物

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Certificate of Analysis (CoA)

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Related Products
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free
NHEK-Neo – Human Epidermal Keratinocytes – Neonatal, Single Donor 00192907
NHEK-Neo – 人类表皮角质形成细胞 – 新生儿，单一供体 00192907
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free
NHEK-Ad – Human Epidermal Keratinocytes, Adult, Single Donor 00192627
NHEK-Ad – 人表皮角质形成细胞，成人，单一供体 00192627
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free
NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled 00192906
NHEK-Neo – 人表皮角质形成细胞，新生儿，汇集 00192906
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free
D-HEK-Ad – Diseased Human Epidermal Keratinocytes, Adult – Diabetes Type II CC-2926
D-HEK-Ad – 患病的人表皮角质形成细胞，成人 – II 型糖尿病 CC-2926
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free
KGM™ Gold Keratinocyte Growth Medium BulletKit™, Calcium Free 00195769
KGM™ Gold 角质形成细胞生长培养基 BulletKit™，无钙 00195769
Data
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free

Morphology of Lonza’s Adult Keratinocytes Grown in KGM™ Gold Growth Medium Compared to Competitor A’s Adult Keratinocytes in Their Medium.

The growth performance and longevity of Competitor A’s adult keratinocytes grown in Competitor A’s Medium were compared with Lonza’s adult keratinocytes grown in KGM-Gold™ Growth Medium. Although Competitor A claims ≥25 total population doublings for their adult keratinocytes, actual results show that this lot barely obtained 19 PD. Note the presence of several enlarged cells in Passage 2 in Competitor’s Medium. At the end of passage 4 (15 PD) Lonza’s adult keratinocytes consist of mostly small proliferative cells.

lonzaCC-3217,原代细胞和干细胞,CBM™ 软骨细胞生长基础培养基, CGM™ CC-3216

货号: CC-3217
CBMTM Chondrocyte Growth Basal Medium
CBMTM 软骨细胞生长基础培养基

Product Overview

CBMTM is a Chondrocyte cell growth basal medium sold separately. This can be used with 1 kit of CC-4409 (chondrocyte cell growth medium SingleQuotsTM Supplements and Growth Factors). The full CGMTM BulletKitTM consisting of basal medium and supplements can be purchased together using the catalog number CC-3216.

  • CGMTM Chondrocyte Growth Medium is specially designed for expansion of chondrocytes. The BulletKitTM is conveniently packaged with basal medium and supplements included for the growth of chondrocytes.
  • CDMTM Chondrocyte Differentiation Medium is also available and sold separately for differentiation of human chondrocytes

CBMTM 是一种软骨细胞生长基础培养基，单独出售。 这可以与 1 套 CC-4409（软骨细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。 由基础培养基和补充剂组成的完整 CGMTM BulletKitTM 可以使用目录号 CC-3216 一起购买。

CGMTM 软骨细胞生长培养基专为软骨细胞的扩增而设计。 BulletKitTM 方便地与基础培养基和用于软骨细胞生长的补充剂一起包装。
CDMTM 软骨细胞分化培养基也可单独购买和出售，用于分化人软骨细胞

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Certificate of Analysis (CoA)

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Related Products

CBM™ Chondrocyte Growth Basal Medium
NHAC-kn – Human Knee Articular Chondrocytes CC-2550
NHAC-kn – 人膝关节软骨细胞 CC-2550
CBM™ Chondrocyte Growth Basal Medium
CDM™ Chondrocyte Differentiation Medium BulletKit™ CC-3225
CDM™ 软骨细胞分化培养基 BulletKit™ CC-3225
CBM™ Chondrocyte Growth Basal Medium
CGM™ Chondrocyte Growth Medium BulletKit™ CC-3216
 CGM™ 软骨细胞生长培养基 BulletKit™ CC-3216

lonza3W-300,lonza2W-300,原代细胞和干细胞,新鲜人 CD8+ 细胞毒性 T 细胞

货号: 3W-300
Human CD8+ cytotoxic T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 10 million cells, fresh
人 CD8+ 细胞毒性 T 细胞，使用负免疫磁性选择从外周血中分离，≥ 1000 万个细胞，新鲜

Product Overview

Leukapheresed CD8+ Cytotoxic T cells are negatively selected from MNC using an indirect immunomagnetic CD8+ T cell labeling system. Cells are shipped in a centrifuge tube. Cell purity is determined using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols. Manufactured by AllCells® Additional shipping charges apply for fresh, human origin products

使用间接免疫磁性 CD8+ T 细胞标记系统从 MNC 中阴性选择 Leukapheresed CD8+ 细胞毒性 T 细胞。 细胞在离心管中运输。 使用 FACS 测定法确定细胞纯度。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞。 由 AllCells® 制造 新鲜的人源产品需支付额外运费

Content & Storage

Content

1 vial containing ≥ 10 million fresh cells

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Certificate of Analysis (CoA)

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Related Products

Fresh Human CD8+ Cytotoxic T Cells
Human Peripheral Blood CD8+ T Cells, Cryopreserved, 10 million cells 2W-300
人外周血 CD8+ T 细胞，冷冻保存，1000 万个细胞 2W-300

lonza,lonzaCC-4398,原代细胞和干细胞,抗坏血酸0.5 mL

货号: CC-4398
Ascorbic Acid, 0.5 mL

Product Overview

Ascorbic Acid is an important component in various primary cell culture media kits. It is included in various BulletKits. Ascorbic Acid is also sold separately at a concentration of 70 mM

抗坏血酸是各种原代细胞培养基试剂盒中的重要成分。 它包含在各种 BulletKits 中。 抗坏血酸也单独出售，浓度为 70 mM

Content & Storage

Content

1 x Vial Containing 70 mM Ascorbic Acid 0.5 mL

Instructions, SDS, CoA

Ascorbic Acid, 0.5 mL

Safety Data Sheets (SDS)

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lonza,lonza3M-103,原代细胞和干细胞,未加工的人骨髓, Fresh, 3 mL

Catalog #: 3M-103
Fresh Unprocessed Human Bone Marrow, 3 mL
新鲜未加工的人骨髓

Product Overview

Bone marrow aspirate is collected from healthy donors and contains 80 IU/mL (nominal) porcine heparin as an anticoagulant. The fresh tissue contains CD34+ hematopoietic stem and progenitor cells (HSPC) and mesenchymal stem/stromal cells (MSC). CD34+ HSPC have the potential to self-renew and differentiate into all the mature blood cell lineages. MSC have the potential to differentiate into several lineages including bone, cartilage, muscle, and fat. Cells are collected in a transport tube.

Manufactured by AllCells®

Additional shipping charges apply for fresh, human origin products

从健康供体收集的骨髓抽吸物含有 80 IU/mL（标称）猪肝素作为抗凝剂。 新鲜组织含有 CD34+ 造血干细胞和祖细胞 (HSPC) 和间充质干/基质细胞 (MSC)。 CD34+ HSPC 具有自我更新和分化成所有成熟血细胞谱系的潜力。 MSC 有可能分化成几个谱系，包括骨骼、软骨、肌肉和脂肪。 将细胞收集在运输管中。

由 AllCells® 制造

新鲜的人源产品需支付额外的运费

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis (CoA) is provided for each cell lot purchased
  • 支原体、细菌、酵母菌和真菌检测呈阴性
    并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎
    为购买的每个细胞批次提供分析证书 (CoA)

Content & Storage

Content

1 x Unprocessed Human Bone Marrow, 3 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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lonza,lonza R-RET-508,原代细胞和干细胞,大鼠新生儿视网膜细胞,BulletKit™ CC-4461

货号: R-RET-508

Cryopreserved ampule of Rat Neonatal Retinal Cells containing ≥200,000 cells

含有≥200,000 个细胞的大鼠新生儿视网膜细胞冻存安瓿

Product Overview

CloneticsTM Neonatal Rat Retinal Cells [P3-4] are ready-to-use, high quality primary cells from Sprague Dawley rats. Cells are prepared by standardized methods and are ready for immediate culture upon thaw. Cells are cryopreserved with ≥ 0.2M viable cells per ampule.

Cells are characterized by immunostaining for neuron specific class III β-tubulin (Tuj-1), specific neuronal protein gene product (PGP 9.5), ganglion cell marker, Thy1.1 and GFAP.

Recommended medium: PNGMTM BulletKitTM

CloneticsTM 新生大鼠视网膜细胞 [P3-4] 是来自 Sprague Dawley 大鼠的即用型高质量原代细胞。 细胞通过标准化方法制备，解冻后即可立即培养。 细胞以每安瓿 ≥ 0.2M 活细胞冷冻保存。

细胞的特征在于对神经元特异性 III 类 β-微管蛋白 (Tuj-1)、特异性神经元蛋白基因产物 (PGP 9.5)、神经节细胞标记物、Thy1.1 和 GFAP 进行免疫染色。

推荐介质：PNGMTM BulletKitTM

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • General ophthalmic research
  • Posterior segment disease
  • Inflammation
  • Degeneration
  • Neoplasms
  • Drug delivery
  • Cell therapies
  • Toxicology and cytotoxicity
  • Gene expression

Content & Storage

Content

≥200,000 cryopreserved cells per ampule

Instructions, SDS, CoA

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Related Products

Rat Neonatal Retinal Cells
PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461
PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

Rat Neonatal Retinal Cells

lonza,lonza 00195406,原代细胞和干细胞,RtEBM™ 视网膜上皮细胞基础培养基

Catalog #: 00195406

Retinal Epithelial Cell Basal Medium, 200 mL

视网膜上皮细胞基础培养基，200 mL

Product Overview

RtEBMTM is a Retinal Epithelial Cell basal medium sold separately. This can be used with 1 kit of 195407 (retinal epithelial cell growth medium SingleQuotsTM Supplements and Growth Factors). The full RtEGMTM BulletKitTM consisting of basal medium and supplements can be purchased together using the catalog number 195409.

RtEGMTM Retinal Pigment Epithelial Cell Growth Medium is optimized and performance tested for growing Human RPE cells. It contains 2% serum.

RtEBMTM 是一种视网膜上皮细胞基础培养基，单独出售。 这可以与 1 套 195407（视网膜上皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。 可以使用目录号 195409 一起购买由基础培养基和补充剂组成的完整 RtEGMTM BulletKitTM。

RtEGMTM 视网膜色素上皮细胞生长培养基针对生长的人类 RPE 细胞进行了优化和性能测试。 它含有2％的血清。

Benefits

Applications:

  • Age-related macular degeneration
  • Retinitis pigmentosa
  • Diabetic retinopathy
  • Photoreceptor degeneration
  • Gene expression
  • Cellular interactions

Content & Storage

Content

1 x Bottle Basal Medium 200 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Related Products

RtEBM™ Retinal Epithelial Cell Basal Medium
RtEGM™ Retinal Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors 00195407
RtEGM™ 视网膜上皮细胞生长培养基 SingleQuots™ 添加剂和生长因子 00195407
RtEBM™ Retinal Epithelial Cell Basal Medium
H-RPE – Human Retinal Pigment Epithelial Cells 00194987
H-RPE – 人视网膜色素上皮细胞 00194987
RtEBM™ Retinal Epithelial Cell Basal Medium
RtEGM™ Retinal Pigment Epithelial Cell Growth Medium BulletKit™ 00195409
 RtEGM™ 视网膜色素上皮细胞生长培养基 BulletKit™ 00195409

lonza,lonza CC-3210,原代细胞和干细胞,NPBM™ 神经祖细胞基础培养基

货号: CC-3210
NPBMTM Medium, 200 mL

Product Overview

PoieticsTM Neural Progenitor Cell System contains Norman Human Neural Progenitor Cells (NHNP) and defined medium optimized for their growth and in vitro differentiation. This system may be used for experimental applications including drug development, neurotoxicity, neurogenesis, electrophysiology, neurotransmitter disorders, and central nervous system function.

The available defined neural progenitor media includes a growth medium BulletKitTM (CC-3209) as well as a differentiaion medium BulletKitTM (CC-3229).

PoieticsTM Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system.

PoieticsTM 神经祖细胞系统包含诺曼人神经祖细胞 (NHNP) 和针对其生长和体外分化优化的确定培养基。 该系统可用于实验应用，包括药物开发、神经毒性、神经发生、电生理学、神经递质疾病和中枢神经系统功能。

可用的定义神经祖细胞培养基包括生长培养基 BulletKitTM (CC-3209) 以及分化培养基 BulletKitTM (CC-3229)。

PoieticsTM 细胞、培养基和试剂一起经过质量测试，保证作为一个完整的细胞系统提供最佳性能。

Content & Storage

Content

  •  Neural Progenitor Basal Medium (1 bottle)
Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Related Products

NPBM™ Neural Progenitor Basal Medium
NHNP – Human Neural Progenitor Cells PT-2599
NHNP – 人类神经祖细胞 PT-2599

lonza,lonza CC-3229,原代细胞和干细胞,NPDM™ 神经祖细胞分化培养基 BulletKit™

货号: CC-3229
NPDM BulletKitTM
lonza,lonza CC-3229,原代细胞和干细胞,NPDM™ 神经祖细胞分化培养基 BulletKit™

Product Overview

PoieticsTM Neural Progenitor Cell System contains Norman Human Neural Progenitor Cells (NHNP) and defined medium optimized for their growth and in vitro differentiation. This system may be used for experimental applications including drug development, neurotoxicity, neurogenesis, electrophysiology, neurotransmitter disorders, and central nervous system function.

The available defined neural progenitor media includes a growth medium BulletKitTM (CC-3209) as well as a differentiaion medium BulletKitTM (CC-3229).

PoieticsTM Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system.

Instructions, SDS, CoA

PoieticsTM 神经祖细胞系统包含诺曼人神经祖细胞 (NHNP) 和针对其生长和体外分化优化的确定培养基。 该系统可用于实验应用，包括药物开发、神经毒性、神经发生、电生理学、神经递质疾病和中枢神经系统功能。

可用的定义神经祖细胞培养基包括生长培养基 BulletKitTM (CC-3209) 以及分化培养基 BulletKitTM (CC-3229)。

PoieticsTM 细胞、培养基和试剂一起经过质量测试，保证作为一个完整的细胞系统提供最佳性能。

说明书、SDS、CoA

Safety Data Sheets (SDS)

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Related Products
NPDM™ Neural Progenitor Differentiation Medium BulletKit™
NHNP – Human Neural Progenitor Cells PT-2599
NHNP – 人类神经祖细胞 PT-2599

lonza,lonza RSCP01,原代细胞和干细胞,大鼠 Sprague Dawley 肝细胞

Catalog #: RSCP01

Rat Plateable Cryopreserved Hepatocytes are specifically tested for healthy morphology lasting 72 hours in monolayer sandwich culture.

大鼠可平板冻存肝细胞在单层夹心培养中经过 72 小时的健康形态学专门测试。

Product Overview

Cryopreserved Sprague Dawley Rat Hepatocytes, plateable qualified  are specifically pre-tested for metabolic activity of general Phase I and Phase II metabolism in suspension. They are tested for healthy morphology lasting 72 hours in monolayer sandwich culture.

Many batches are talso ested for spheroid formation capacity.  Look for the Verified for SpheroidsTMcharacteristic on each CofA

冷冻保存的 Sprague Dawley 大鼠肝细胞，可电镀合格，专门针对悬浮液中一般 I 期和 II 期代谢的代谢活性进行了预测试。 它们在单层夹层培养中经过 72 小时的健康形态测试。

还评估了许多批次的球体形成能力。 在每个 CofA 上查找 Verified for SpheroidsTMcharacteristic

Benefits

  • Plateable
  • >5Million cells/vial
  • Characterized for general drug metabolism

Applications

  • Drug metabolism in plated monolayer format
  • species-species comparisons
  • genomics

Content & Storage

Content

Cryo vial, > 5 million viable cells

Instructions, SDS, CoA

Related Products

Rat Sprague Dawley Hepatocytes, Cryopreserved, Plateable
Human Hepatocytes, Cryopreserved, Plateable HUCPG

人肝细胞，冷冻保存，可电镀 HUCPG

Rat Sprague Dawley Hepatocytes, Cryopreserved, Plateable
Human Hepatocytes, Cryopreserved, Plateable and Interaction Qualified HUCPI

Rat Sprague Dawley Hepatocytes, Cryopreserved, Plateable

人肝细胞，冷冻保存，可电镀和相互作用合格的 HUCPI
Rat Sprague Dawley Hepatocytes, Cryopreserved, Plateable

lonza,lonza M-IFB-482,原代细胞和干细胞,辐照小鼠胚胎成纤维细胞 (iMEF)

Catalog #: M-IFB-482

Cryopreserved ampule of Irradiated Mouse Embryonic Fibroblasts containing ≥2 million cells

冷冻保存的辐照小鼠胚胎成纤维细胞安瓿，含有≥200万个细胞

Product Overview

Irradiated Mouse primary embryonic fibroblasts (iMEF) are dissociated from day 14 and 15 CD1 mouse embryos, expanded and then inactivated using gamma radiation.

Each vial contains at least 2 million cells. iMEF are convenient and easy to use “feeder cells” commonly co-cultured with embryonic and pluripotent stem cell cultures.

Recommended Media: Lonza’s DMEM High Glucose w/ L-Gln, 500 mL (Part 12-604F) containing 10% FBS (Sigma-Aldrich Part F6178)

经辐照的小鼠原代胚胎成纤维细胞 (iMEF) 从第 14 天和第 15 天的 CD1 小鼠胚胎中分离出来，扩增然后使用伽马辐射灭活。

每个小瓶包含至少 200 万个细胞。 iMEF 是方便且易于使用的“饲养细胞”，通常与胚胎和多能干细胞培养物共同培养。

推荐培养基：Lonza’s DMEM High Glucose w/ L-Gln，500 mL（第 12-604F 部分），含 10% FBS（Sigma-Aldrich 第 F6178 部分）

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Serve as “feeder cell” monolayer in embryonic (ES) and induced pluripotent (iPSC) stem cell cultures
  • Secrete important growth factors into culture medium to support ES and iPSC undifferentiated state
  • Act as cellular matrix to support ES cell growth
  • 在胚胎 (ES) 和诱导多能 (iPSC) 干细胞培养物中充当“饲养细胞”单层
    将重要的生长因子分泌到培养基中以支持 ES 和 iPSC 未分化状态
    作为细胞基质支持 ES 细胞生长

Content & Storage

Content

≥ 2 million cryopreserved, gamma irradiated cells per ampule

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

 

 

Related Products
Irradiated Mouse Embryonic Fibroblasts (iMEF)
HEPES Buffered Saline Solution – 100 ml CC-5022

HEPES 缓冲盐溶液 – 100 ml CC-5022

lonza,lonza CC-3103,原代细胞和干细胞,KBM™- 2 角质形成细胞基础培养基-2

Catalog #: CC-3103
KBMTM 2 Keratinocyte Basal Medium-2, 500 mL
KBMTM 2 角质形成细胞基础培养基-2，500 mL

Product Overview

KGM-2 BulletkitTM, also sold as separate basal medium and supplements

KGM-2 BulletkitTM，也作为单独的基础培养基和补充剂出售

Product Overview:

CloneticsTM KGMTM-2 Keratinocyte Growth Medium-2 has been optimized for the proliferation of keratinocytes in a serum-free environment and typically provides higher growth rates than KGMTM Medium.

CloneticsTM Media Products are specially designed to support the growth of human and animal primary derived cells. Media systems have been specifically developed and optimized for various cell systems. Media are supplied in several formats:

  • Basal Medium – contains no growth factors cytokines, or supplements
  • SingleQuotsTM Kit – growth factors, cytokines, and supplements
  • BulletKitTM – basal medium and SingleQuotsTM Kit packaged together

CloneticsTM KGMTM-2 角质形成细胞生长培养基-2 已针对在无血清环境中的角质形成细胞增殖进行了优化，通常提供比 KGMTM 培养基更高的生长速率。

CloneticsTM 培养基产品专为支持人和动物原代细胞的生长而设计。 媒体系统已针对各种细胞系统专门开发和优化。 媒体以多种格式提供：

基础培养基 – 不含生长因子细胞因子或补充剂

SingleQuotsTM 试剂盒——生长因子、细胞因子和补充剂

BulletKitTM – 基础培养基和 SingleQuotsTM 套件包装在一起


Get more information on the discontinuation of certain keratinocytes products.

KBM™- 2 Keratinocyte Basal Medium-2

Safety Data Sheets (SDS)

View SDS

SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.