Dojindo,氨基-EG6-十一烷硫醇-盐酸盐/10/A483

Product Description

聚乙二醇 (PEG) 广泛用于材料改性以提高表面的亲水性。 PEG 涂层材料通常在生理条件下更稳定。 由于氨基-EG6-十一硫醇具有6个乙二醇单元，11个碳原子，末端有一个SH基团，可用于在金表面制备高度取向和亲水的SAM。 由于提高了亲水性，这适用于表面上的生物材料标记。 亲水表面可以防止蛋白质或其他生物材料发生非特异性结合。 因此，该试剂制备的SAM将为开发生物材料传感器或DNA/蛋白质微阵列提供更好的表面。 为了在金表面上制备氨基-EG6-SAM，羟基-EGn-十一硫醇（n = 3, 6）用于根据引入到表面上的分子的密度来稀释氨基的数量。

How to Prepare SAM1. Soak a gold-coated glass plate in Piranha solutiona) for 10-15 minutes. Wash the plate with purified water.^a)2. Dissolve aminoalkanethiol compound in ethanol to prepare several mM to several ten mM solutions.3. Soak the plate in the aminoalkanethiol solution for a certain time period.^b)4. Wash the SAM-coated plate with ethanol and then water.5. Dry the plate under nitrogen atmosphere, if necessary.

a)Piranha solution: sulfuric acid and 30% hydrogen peroxide, 3:1. Piranha solution is a strong oxidizing agent. Extreme care is necessary when using it.Do not apply Piranha solution to resin-coated plates; it may erode the resin.b)To prepare a SAM-coated plate with the best performance, aminoalkanethiol concentration and soaking time should be individually determined.

Application of SAM-Preparation of DNA Array (Fig. 1)1. Use SF10 glass slides (Schott Glass Technologies) coated with 5 nm chromium and 45 nm gold thin film.2. Soak the glass slide in a 1 mM 1-octadecanethiol (ODT)/ethanol solution overnight to prepare ODT SAM-coated slide.3. Draw 500 μm x 500 μm patterns on the ODT SAM-coated slide by UV irradiation with an Hg-Xe arc lamp.^a)4. Soak the slide in a 1 mM 11-amino-1-undecanethiol (AUT)/ethanol solution for 2 hours to form AUT SAM on the 500 μm x 500 μm photopatterned area.5. Drop 2 mM SPDP solution^b) onto the slide and leave the slide at room temperature.6. Wash the slide and dry under nitrogen atmosphere.7. Apply 1 mM thiol-DNA solution^c) to each 500 μm x 500 μm pattern and incubate at room temperature overnight.8. Incubate the slide with a sample solution for 10 minutes and wash with phosphate buffer, followed by SPR imaging.

a)Irradiation time: 1-1.5 hoursb)SPDP: N-succinimidyl 3-(2-pyridyldithio)propionate. Dissolve SPDP in DMSO to prepare 50 mM solution. Dilute it 25 times with 100 mM triethanolamine buffer, pH 7.0.c)Dissolve thiol-DNA with 100 mM triethanolamine buffer, pH 8.0.